Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPACEI0381(ACE-inhibitory peptide)

DFBP ID	DFBPACEI0381
Peptide sequence	YGFLP
Type	Native peptide
Peptide/Function name	ACE-inhibitory peptide

Function-activity relationship

Main bioactivity	ACE-inhibitory activity
Otheir bioactivity	Opioid activity ^[D1], Multifunctional activity ^[D2]

Calculated physicochemical properties

Three-letter amino acid

Tyr-Gly-Phe-Leu-Pro

Single-letter amino acid

YGFLP

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	595.69 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

5.92 ^c

IC₅₀

260 μM

pIC₅₀

-2.415

GRAVY

0.6600^c

Hydrophilic residue ratio

80%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Human
Organism/Source	Human milk protein
Precursor protein	β-Casein
Residue position	f(59-63)
Precursor protein(s) search	Source.1: DFBPPR2000 ---- Plant proteins ---- Sodium/calcium exchanger NCL1 Source.2: DFBPPR2164 ---- Plant proteins ---- Sodium/calcium exchanger NCL2 Source.3: DFBPPR4837 ---- Plant proteins ---- Protein DEHYDRATION-INDUCED 19 homolog 5 Source.4: DFBPPR7605 ---- Milk proteins ---- Beta-casein Source.5: DFBPPR17137 ---- Animal proteins ---- Interleukin-1 receptor-associated kinase 1 Source.6: DFBPPR19770 ---- Animal proteins ---- Heat shock 70 kDa protein 13 Source.7: DFBPPR20543 ---- Animal proteins ---- Procollagen galactosyltransferase 1 Source.8: DFBPPR15421 ---- Microorganism protein ---- Cytochrome c lysine N-methyltransferase 1
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

ACE-inhibitory activity	The peptide exhibited moderate Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) inhibitory activity with an IC₅₀ value of 260 uM.
Specific target protein(s)	Specific Target Protein(s): Angiotensin-converting enzyme

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(Cc1ccc(O)cc1)C(=O)NCC(=O)N[C@@]([H])(Cc1ccccc1)C(=O)N[C@@]([H])(CC(C)C)C(=O)N1[C@@]([H])(CCC1)C(=O)O

Preparation method

Mode of preparation	Synthesis
Enzyme(s)/starter culture	The peptides used in this study were synthesized by the conventional liquid-phase method.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

DFBP

[D1]	DFBPOPIO0042
[D2]	DFBPMUFU0406

[D3]

[D4]

[D5]

[D6]

Reference(s)

Primary literature	Kohmura, M., Nio, N., Kubo, K., Minoshima, Y., Munekata, E., Ariyoshi, Y. Inhibition of Angiotensin-converting Enzyme by Synthetic Peptides of Human β-Casein. Agricultural and Biological Chemistry. 2014, 53, 2107-14. DOI: 10.1080/00021369.1989.10869621
Other literature(s)	[1] Yoshikawa M, Tani F, Yoshimura T, et al. Opioid Peptides from Milk Proteins[J]. Journal of the Agricultural Chemical Society of Japan, 1986, 50(9):2419-2421.
PubDate	2014