Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPACEI0500(ACE-inhibitory peptide)

DFBP ID	DFBPACEI0500
Peptide sequence	LQSW
Type	Native peptide
Peptide/Function name	ACE-inhibitory peptide

Function-activity relationship

Main bioactivity	ACE-inhibitory activity
Otheir bioactivity	Antihypertensive activity ^[D1], Multifunctional activity ^[D2]

Calculated physicochemical properties

Three-letter amino acid

Leu-Gln-Ser-Trp

Single-letter amino acid

LQSW

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	532.59 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

5.97 ^c

IC₅₀

500 μM

pIC₅₀

-2.699

GRAVY

-0.3500^c

Hydrophilic residue ratio

50%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Milk protein
Precursor protein	β-Casein
Residue position	f(140-143)
Precursor protein(s) search	Source.1: DFBPPR0855 ---- Plant proteins ---- LRR receptor kinase BAK1 Source.2: DFBPPR0871 ---- Plant proteins ---- LRR receptor kinase SERK2 Source.3: DFBPPR1567 ---- Plant proteins ---- Protein argonaute PNH1 Source.4: DFBPPR1616 ---- Plant proteins ---- Anthranilate synthase alpha subunit 2, chloroplastic Source.5: DFBPPR1800 ---- Plant proteins ---- APETALA2-like protein 4 Source.6: DFBPPR2113 ---- Plant proteins ---- Neutral/alkaline invertase 1, mitochondrial Source.7: DFBPPR2206 ---- Plant proteins ---- Leucine-rich repeat protein 1 Source.8: DFBPPR3648 ---- Plant proteins ---- Putative lipoxygenase 5 Source.9: DFBPPR3675 ---- Plant proteins ---- Neutral/alkaline invertase 3, chloroplastic Source.10: DFBPPR7653 ---- Milk proteins ---- Signal transducer and activator of transcription 5A Source.11: DFBPPR7693 ---- Milk proteins ---- Signal transducer and activator of transcription 5A Source.12: DFBPPR7718 ---- Milk proteins ---- Beta-casein Source.13: DFBPPR8385 ---- Plant proteins ---- Alpha-methyl-mannoside-specific lectin Source.14: DFBPPR8489 ---- Milk proteins ---- Beta-casein Source.15: DFBPPR8506 ---- Milk proteins ---- Signal transducer and activator of transcription 5A Source.16: DFBPPR16622 ---- Animal proteins ---- EGF domain-specific O-linked N-acetylglucosamine transferase Source.17: DFBPPR16761 ---- Animal proteins ---- NADP-dependent malic enzyme Source.18: DFBPPR19114 ---- Animal proteins ---- Protein C-ets-2 Source.19: DFBPPR20270 ---- Animal proteins ---- EGF domain-specific O-linked N-acetylglucosamine transferase Source.20: DFBPPR20702 ---- Animal proteins ---- 5-azacytidine-induced protein 2 Source.21: DFBPPR21301 ---- Animal proteins ---- Lymphocyte antigen 6D Source.22: DFBPPR21767 ---- Animal proteins ---- Tudor domain-containing protein 5 Source.23: DFBPPR22155 ---- Animal proteins ---- IQ domain-containing protein F1 Source.24: DFBPPR22649 ---- Animal proteins ---- IQ domain-containing protein F5 Source.25: DFBPPR8610 ---- Animal proteins ---- Signal transducer and activator of transcription 5B Source.26: DFBPPR9066 ---- Animal proteins ---- Aminoacylase-1 Source.27: DFBPPR10577 ---- Animal proteins ---- Frizzled-10 Source.28: DFBPPR10927 ---- Animal proteins ---- Frizzled-7 Source.29: DFBPPR11030 ---- Animal proteins ---- Protein C-ets-2 Source.30: DFBPPR11280 ---- Animal proteins ---- KICSTOR complex protein kaptin Source.31: DFBPPR11385 ---- Animal proteins ---- EGF domain-specific O-linked N-acetylglucosamine transferase Source.32: DFBPPR12257 ---- Animal proteins ---- Polyunsaturated fatty acid lipoxygenase ALOX15 Source.33: DFBPPR12559 ---- Animal proteins ---- Peroxisomal acyl-coenzyme A oxidase 2 Source.34: DFBPPR15016 ---- Microorganism protein ---- Very-long-chain 3-oxoacyl-CoA reductase Source.35: DFBPPR15096 ---- Microorganism protein ---- Acetyl-coenzyme A synthetase 2 Source.36: DFBPPR15745 ---- Microorganism protein ---- Protein FYV8
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

ACE-inhibitory activity	The peptide exhibited low Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) inhibitory activity with an IC₅₀ value of 500 μM.
Specific target protein(s)	Specific Target Protein(s): Angiotensin-converting enzyme

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	No prediction can be made about the peptide bitterness. ^prediction

SMILES

N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)N[C@@]([H])(CO)C(=O)N[C@@]([H])(CC(=CN2)C1=C2C=CC=C1)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Milk proteins were hydrolyzed by an extracellular proteinase from Lactobacillus helveticus CP790.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

DFBP

[D1]	DFBPANHY0557
[D2]	DFBPMUFU0126

[D3]

[D4]

[D5]

[D6]

Reference(s)

Primary literature	Maeno, M., Yamamoto, N., Takano, T. Identification of an Antihypertensive Peptide from Casein Hydrolysate Produced by a Proteinase from Lactobacillus helveticus CP790. Journal of Dairy Science. 1996, 79, 1316-21. DOI: 10.3168/jds.S0022-0302(96)76487-1
Other literature(s)	[1] Robert, M.C., et al., Identification of angiotensin-I-converting enzyme inhibitory peptides derived from sodium caseinate hydrolysates produced by Lactobacillus helveticus NCC 2765. J Agric Food Chem, 2004. 52(23): p. 6923-31.
PubDate	1996