Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPACEI0820(ACE-inhibitory peptide)

DFBP ID	DFBPACEI0820
Peptide sequence	MDFL
Type	Native peptide
Peptide/Function name	ACE-inhibitory peptide

Function-activity relationship

Main bioactivity	ACE-inhibitory activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Met-Asp-Phe-Leu

Single-letter amino acid

MDFL

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	524.62 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

3.13 ^c

IC₅₀

0.013 mg/L

pIC₅₀

1.886

GRAVY

1.2500^c

Hydrophilic residue ratio

75%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Plant
Organism/Source	Chickpea (Cicer arietinum L.)
Precursor protein	Legumin (seed storage protein)
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR1469 ---- Plant proteins ---- Apoptosis inhibitor 5-like protein API5 Source.2: DFBPPR3365 ---- Plant proteins ---- 50S ribosomal protein L5, chloroplastic Source.3: DFBPPR3587 ---- Plant proteins ---- Auxin transport protein BIG Source.4: DFBPPR3870 ---- Plant proteins ---- Coatomer subunit beta-2 Source.5: DFBPPR4054 ---- Plant proteins ---- Coatomer subunit beta-1 Source.6: DFBPPR5256 ---- Plant proteins ---- Early nodulin-70 Source.7: DFBPPR5383 ---- Plant proteins ---- (E)-beta-caryophyllene synthase Source.8: DFBPPR5785 ---- Plant proteins ---- (E)-beta-caryophyllene synthase Source.9: DFBPPR5835 ---- Plant proteins ---- Dihydroflavonol 4-reductase Source.10: DFBPPR7659 ---- Milk proteins ---- Glycosylation-dependent cell adhesion molecule 1 Source.11: DFBPPR17385 ---- Animal proteins ---- Complement component 1 Q subcomponent-binding protein, mitochondrial Source.12: DFBPPR17988 ---- Animal proteins ---- Poly(A)-specific ribonuclease PARN Source.13: DFBPPR19086 ---- Animal proteins ---- Leucine-rich repeat transmembrane neuronal protein 1 Source.14: DFBPPR19470 ---- Animal proteins ---- Acidic amino acid decarboxylase GADL1 Source.15: DFBPPR19694 ---- Animal proteins ---- Palmitoyltransferase ZDHHC4 Source.16: DFBPPR19813 ---- Animal proteins ---- Phosphatidylinositol 5-phosphate 4-kinase type-2 gamma Source.17: DFBPPR20050 ---- Animal proteins ---- Dihydroxyacetone phosphate acyltransferase Source.18: DFBPPR20652 ---- Animal proteins ---- HAUS augmin-like complex subunit 1 Source.19: DFBPPR21778 ---- Animal proteins ---- Lysosomal-associated transmembrane protein 5 Source.20: DFBPPR21871 ---- Animal proteins ---- Serpin B10 Source.21: DFBPPR22321 ---- Animal proteins ---- Solute carrier family 25 member 35 Source.22: DFBPPR8684 ---- Animal proteins ---- Sialoadhesin Source.23: DFBPPR8726 ---- Animal proteins ---- Serine-protein kinase ATM Source.24: DFBPPR8819 ---- Animal proteins ---- H(+)/Cl(-) exchange transporter 5 Source.25: DFBPPR10119 ---- Animal proteins ---- Down syndrome cell adhesion molecule homolog Source.26: DFBPPR10343 ---- Animal proteins ---- Cytochrome P450 2H1 Source.27: DFBPPR10486 ---- Animal proteins ---- Cytochrome P450 2H2 Source.28: DFBPPR10696 ---- Animal proteins ---- pre-rRNA 2'-O-ribose RNA methyltransferase FTSJ3 Source.29: DFBPPR10871 ---- Animal proteins ---- Carnosine N-methyltransferase 2 Source.30: DFBPPR10964 ---- Animal proteins ---- Protein artemis Source.31: DFBPPR11231 ---- Animal proteins ---- 116 kDa U5 small nuclear ribonucleoprotein component Source.32: DFBPPR11276 ---- Animal proteins ---- tRNA wybutosine-synthesizing protein 5 Source.33: DFBPPR11410 ---- Animal proteins ---- Target of Myb protein 1 Source.34: DFBPPR11611 ---- Animal proteins ---- Potassium channel subfamily T member 1 Source.35: DFBPPR12131 ---- Animal proteins ---- Exportin-4 Source.36: DFBPPR12589 ---- Animal proteins ---- H(+)/Cl(-) exchange transporter 5 Source.37: DFBPPR12801 ---- Animal proteins ---- Cytochrome P450 3A6 Source.38: DFBPPR14797 ---- Marine protein ---- Gonad-inhibiting hormone Source.39: DFBPPR15232 ---- Microorganism protein ---- U3 small nucleolar RNA-associated protein 10 Source.40: DFBPPR15653 ---- Microorganism protein ---- Conserved oligomeric Golgi complex subunit 6 Source.41: DFBPPR8215 ---- Plant protein ---- Eupatolide synthase
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

ACE-inhibitory activity	The peptide exhibited potent Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) inhibitory activity with an IC₅₀ value of 0.013 mg/L.
Specific target protein(s)	Specific Target Protein(s): Angiotensin-converting enzyme

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(CCSC)C(=O)N[C@@]([H])(CC(=O)O)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)N[C@@]([H])(CC(C)C)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Chickpea legumin was hydrolyzed with the protease Alcalase 2.4 L.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

The hydrolysates of chickpea legumin obtained by treatment with alcalase are a good source of peptides with angiotensin I-converting enzyme inhibitory activity.

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Yust, M., x, a, M., Pedroche, J., Girón-Calle, J., Alaiz, M., et al. Production of ace inhibitory peptides by digestion of chickpea legumin with alcalase. Food Chem. 2003, 81, 363-9. DOI: 10.1016/S0308-8146(02)00431-4
Other literature(s)	[1] Murray, B.A. and R.J. FitzGerald, Angiotensin converting enzyme inhibitory peptides derived from food proteins: biochemistry, bioactivity and production. Curr Pharm Des, 2007. 13(8): p. 773-91.
PubDate	2003