Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPACEI0822(ACE-inhibitory peptide)

DFBP ID	DFBPACEI0822
Peptide sequence	YNKL
Type	Native peptide
Peptide/Function name	ACE-inhibitory peptide

Function-activity relationship

Main bioactivity	ACE-inhibitory activity
Otheir bioactivity	Antihypertensive activity ^[D1], Multifunctional activity ^[D2]

Calculated physicochemical properties

Three-letter amino acid

Tyr-Asn-Lys-Leu

Single-letter amino acid

YNKL

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	536.62 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

9.70 ^c

IC₅₀

21 uM

pIC₅₀

-1.322

GRAVY

-1.2250^c

Hydrophilic residue ratio

25%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Marine
Organism/Source	Wakame (Undaria pinnatifida)
Precursor protein	Wake hydrolysates
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR0872 ---- Plant proteins ---- Beta-glucosidase 6 Source.2: DFBPPR1091 ---- Plant proteins ---- LRR receptor-like serine/threonine-protein kinase ER1 Source.3: DFBPPR1617 ---- Plant proteins ---- DNA replication licensing factor MCM7 Source.4: DFBPPR1724 ---- Plant proteins ---- Protein disulfide isomerase-like 1-4 Source.5: DFBPPR1758 ---- Plant proteins ---- MADS-box transcription factor 57 Source.6: DFBPPR2276 ---- Plant proteins ---- Probable calcium-transporting ATPase 6, plasma membrane-type Source.7: DFBPPR2722 ---- Plant proteins ---- DEAD-box ATP-dependent RNA helicase 20 Source.8: DFBPPR3053 ---- Plant proteins ---- Beta-glucosidase 18 Source.9: DFBPPR3088 ---- Plant proteins ---- Beta-glucosidase 34 Source.10: DFBPPR3371 ---- Plant proteins ---- Kinesin-like protein KIN-14R Source.11: DFBPPR4148 ---- Plant proteins ---- Disease resistance protein PIK5-NP Source.12: DFBPPR4434 ---- Plant proteins ---- Probable metal-nicotianamine transporter YSL18 Source.13: DFBPPR4940 ---- Plant proteins ---- Protein STAY-GREEN, chloroplastic Source.14: DFBPPR5173 ---- Plant proteins ---- Stem 31 kDa glycoprotein Source.15: DFBPPR5223 ---- Plant proteins ---- Stem 28 kDa glycoprotein Source.16: DFBPPR5259 ---- Plant proteins ---- Stem 31 kDa glycoprotein Source.17: DFBPPR6628 ---- Plant proteins ---- 4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucoside beta-D-glucosidase 1a, chloroplastic Source.18: DFBPPR6629 ---- Plant proteins ---- 4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucoside beta-D-glucosidase 1d, chloroplastic Source.19: DFBPPR6630 ---- Plant proteins ---- 4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucoside beta-D-glucosidase 1b, chloroplastic Source.20: DFBPPR7641 ---- Milk proteins ---- Lactase-phlorizin hydrolase Source.21: DFBPPR16248 ---- Animal proteins ---- Abnormal spindle-like microcephaly-associated protein homolog Source.22: DFBPPR16976 ---- Animal proteins ---- Growth/differentiation factor 8 Source.23: DFBPPR17141 ---- Animal proteins ---- Integrin beta-2 Source.24: DFBPPR17170 ---- Animal proteins ---- A disintegrin and metalloproteinase with thrombospondin motifs 2 Source.25: DFBPPR17489 ---- Animal proteins ---- Adhesion G protein-coupled receptor L2 Source.26: DFBPPR17749 ---- Animal proteins ---- CD9 antigen Source.27: DFBPPR17816 ---- Animal proteins ---- Lumican Source.28: DFBPPR17956 ---- Animal proteins ---- PRKCA-binding protein Source.29: DFBPPR18113 ---- Animal proteins ---- Serine/threonine-protein kinase 38 Source.30: DFBPPR18548 ---- Animal proteins ---- Lipoyl synthase, mitochondrial Source.31: DFBPPR18819 ---- Animal proteins ---- Proteasome activator complex subunit 4 Source.32: DFBPPR19000 ---- Animal proteins ---- Centrosomal protein of 57 kDa Source.33: DFBPPR19043 ---- Animal proteins ---- Threonine--tRNA ligase 1, cytoplasmic Source.34: DFBPPR19086 ---- Animal proteins ---- Leucine-rich repeat transmembrane neuronal protein 1 Source.35: DFBPPR20619 ---- Animal proteins ---- Extracellular matrix protein 2 Source.36: DFBPPR20625 ---- Animal proteins ---- DIS3-like exonuclease 1 Source.37: DFBPPR20712 ---- Animal proteins ---- Melatonin receptor type 1A Source.38: DFBPPR20983 ---- Animal proteins ---- Plakophilin-3 Source.39: DFBPPR21496 ---- Animal proteins ---- Mitochondrial import inner membrane translocase subunit Tim9 Source.40: DFBPPR21875 ---- Animal proteins ---- Coiled-coil domain-containing protein 113 Source.41: DFBPPR8558 ---- Animal proteins ---- Glycerophosphocholine cholinephosphodiesterase ENPP6 Source.42: DFBPPR8714 ---- Animal proteins ---- Integrin beta-2 Source.43: DFBPPR9072 ---- Animal proteins ---- CD9 antigen Source.44: DFBPPR9270 ---- Animal proteins ---- Complement C1s subcomponent Source.45: DFBPPR9956 ---- Animal proteins ---- Fatty acid synthase Source.46: DFBPPR10112 ---- Animal proteins ---- Neuronal acetylcholine receptor subunit beta-2 Source.47: DFBPPR10460 ---- Animal proteins ---- Neuronal acetylcholine receptor subunit beta-4 Source.48: DFBPPR10563 ---- Animal proteins ---- Optineurin Source.49: DFBPPR10607 ---- Animal proteins ---- Collagen alpha-2(VI) chain Source.50: DFBPPR10904 ---- Animal proteins ---- Signal transducing adapter molecule 2 Source.51: DFBPPR11134 ---- Animal proteins ---- Keratocan Source.52: DFBPPR11775 ---- Animal proteins ---- Anaphase-promoting complex subunit 5 Source.53: DFBPPR12135 ---- Animal proteins ---- Vigilin Source.54: DFBPPR12190 ---- Animal proteins ---- Transmembrane protein 251 Source.55: DFBPPR12453 ---- Animal proteins ---- Lactase-phlorizin hydrolase Source.56: DFBPPR13097 ---- Animal proteins ---- Lumican Source.57: DFBPPR13116 ---- Animal proteins ---- Ig gamma chain C region Source.58: DFBPPR13432 ---- Animal proteins ---- Integrin beta-2 Source.59: DFBPPR13575 ---- Animal proteins ---- Integrin beta-2 Source.60: DFBPPR13576 ---- Animal proteins ---- Toll-like receptor 9 Source.61: DFBPPR14077 ---- Marine protein ---- Serotransferrin-1 Source.62: DFBPPR14080 ---- Marine protein ---- Serotransferrin-2 Source.63: DFBPPR14853 ---- Marine protein ---- Sarcoplasmic calcium-binding protein 1 Source.64: DFBPPR15061 ---- Microorganism protein ---- AdoMet-dependent rRNA methyltransferase SPB1 Source.65: DFBPPR15064 ---- Microorganism protein ---- Palmitoyltransferase SWF1 Source.66: DFBPPR15279 ---- Microorganism protein ---- Nuclear fusion protein KAR5 Source.67: DFBPPR15382 ---- Microorganism protein ---- Sensitive to high expression protein 9 homolog, mitochondrial Source.68: DFBPPR15783 ---- Microorganism protein ---- Uncharacterized killer plasmid pGKl-2 protein 9 Source.69: DFBPPR8354 ---- Plant protein ---- Pollen-specific protein SF21
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

ACE-inhibitory activity	The peptide exhibited potent Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) inhibitory activity with an IC₅₀ value of 21 μM.
Specific target protein(s)	Specific Target Protein(s): Angiotensin-converting enzyme

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(Cc1ccc(O)cc1)C(=O)N[C@@]([H])(CC(=O)N)C(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(CC(C)C)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	The peptide was separated from the peptic digest of protein prepared from wakame (Undaria pinnatifida).

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

Wakame is the most popular edible seaweed in Japan since ancient times, so that its safety is established. The purified peptide YNKL is a possible candidate for application as a dietary supplement or as a component of a functional food product.

Database cross-references

DFBP

[D1]	DFBPANHY0026
[D2]	DFBPMUFU0356

[D3]

[D4]

[D5]

[D6]

Reference(s)

Primary literature	Suetsuna, K., Nakano, T. Identification of an antihypertensive peptide from peptic digest of wakame (Undaria pinnatifida). The Journal of Nutritional Biochemistry. 2000, 11, 450-4. DOI: 10.1016/s0955-2863(00)00110-8
Other literature(s)	N.D
PubDate	2000