Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPACEI0974(ACE-inhibitory peptide)

DFBP ID	DFBPACEI0974
Peptide sequence	ERAF
Type	Native peptide
Peptide/Function name	ACE-inhibitory peptide

Function-activity relationship

Main bioactivity	ACE-inhibitory activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Glu-Arg-Ala-Phe

Single-letter amino acid

ERAF

Peptide length

Peptide mass

Experimental mass	Theoretical mass
521 Da	521.56 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

6.95 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-0.8500^c

Hydrophilic residue ratio

50%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Plant
Organism/Source	Black Bean (Phaseolus vulgaris)
Precursor protein	Raw Black bean protein hydrolysates
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR0915 ---- Plant proteins ---- Kinesin-like protein KIN-4A Source.2: DFBPPR0939 ---- Plant proteins ---- Protein argonaute MEL1 Source.3: DFBPPR1265 ---- Plant proteins ---- Peptide methionine sulfoxide reductase B1, chloroplastic Source.4: DFBPPR1355 ---- Plant proteins ---- Probable GTP diphosphokinase CRSH3, chloroplastic Source.5: DFBPPR1366 ---- Plant proteins ---- Kinesin-like protein KIN-7F Source.6: DFBPPR1432 ---- Plant proteins ---- Probable GTP diphosphokinase CRSH2, chloroplastic Source.7: DFBPPR1605 ---- Plant proteins ---- Phytochrome B Source.8: DFBPPR1735 ---- Plant proteins ---- Probable aminodeoxychorismate synthase, chloroplastic Source.9: DFBPPR2299 ---- Plant proteins ---- Metal tolerance protein 3 Source.10: DFBPPR2350 ---- Plant proteins ---- Metal tolerance protein 4 Source.11: DFBPPR2355 ---- Plant proteins ---- Probable glycosyltransferase 5 Source.12: DFBPPR2392 ---- Plant proteins ---- Metal tolerance protein 6 Source.13: DFBPPR2401 ---- Plant proteins ---- Kinesin-like protein KIN-4C Source.14: DFBPPR2514 ---- Plant proteins ---- Metal tolerance protein 5 Source.15: DFBPPR2747 ---- Plant proteins ---- Metal tolerance protein 7 Source.16: DFBPPR2762 ---- Plant proteins ---- Serine/threonine-protein phosphatase BSL1 homolog Source.17: DFBPPR2922 ---- Plant proteins ---- Probable glycosyltransferase 2 Source.18: DFBPPR2929 ---- Plant proteins ---- Germin-like protein 2-4 Source.19: DFBPPR3150 ---- Plant proteins ---- Probable glycosyltransferase 3 Source.20: DFBPPR3268 ---- Plant proteins ---- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 Source.21: DFBPPR3334 ---- Plant proteins ---- Probable metal-nicotianamine transporter YSL12 Source.22: DFBPPR3656 ---- Plant proteins ---- Kinesin-like protein KIN-7C Source.23: DFBPPR3671 ---- Plant proteins ---- Putative linoleate 9S-lipoxygenase 3 Source.24: DFBPPR3924 ---- Plant proteins ---- Probable metal-nicotianamine transporter YSL17 Source.25: DFBPPR4033 ---- Plant proteins ---- Urease accessory protein G Source.26: DFBPPR4434 ---- Plant proteins ---- Probable metal-nicotianamine transporter YSL18 Source.27: DFBPPR4494 ---- Plant proteins ---- CRS2-associated factor 1, mitochondrial Source.28: DFBPPR4912 ---- Plant proteins ---- Probable xyloglucan 6-xylosyltransferase 1 Source.29: DFBPPR5953 ---- Plant proteins ---- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 Source.30: DFBPPR6304 ---- Plant proteins ---- Porphobilinogen deaminase, chloroplastic Source.31: DFBPPR6332 ---- Plant proteins ---- Nucleoside diphosphate kinase 1 Source.32: DFBPPR6444 ---- Plant proteins ---- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 Source.33: DFBPPR7040 ---- Plant proteins ---- Linoleate 9S-lipoxygenase 1 Source.34: DFBPPR7234 ---- Plant proteins ---- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 Source.35: DFBPPR7235 ---- Plant proteins ---- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD2 Source.36: DFBPPR17111 ---- Animal proteins ---- Primary amine oxidase, liver isozyme Source.37: DFBPPR17112 ---- Animal proteins ---- Primary amine oxidase, liver isozyme Source.38: DFBPPR18146 ---- Animal proteins ---- Cytochrome b-c1 complex subunit 9 Source.39: DFBPPR18310 ---- Animal proteins ---- Serine/arginine-rich splicing factor 7 Source.40: DFBPPR18311 ---- Animal proteins ---- Protein farnesyltransferase/geranylgeranyltransferase type-1 subunit alpha Source.41: DFBPPR18752 ---- Animal proteins ---- Serine/arginine-rich splicing factor 3 Source.42: DFBPPR18767 ---- Animal proteins ---- Toll-interacting protein Source.43: DFBPPR18921 ---- Animal proteins ---- E3 ubiquitin-protein ligase TRIM11 Source.44: DFBPPR19190 ---- Animal proteins ---- Amiloride-sensitive sodium channel subunit gamma Source.45: DFBPPR19204 ---- Animal proteins ---- Regulator of G-protein signaling 7 Source.46: DFBPPR19496 ---- Animal proteins ---- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 Source.47: DFBPPR19755 ---- Animal proteins ---- Mitochondrial dimethyladenosine transferase 1 Source.48: DFBPPR20272 ---- Animal proteins ---- Fatty acyl-CoA reductase 2 Source.49: DFBPPR20496 ---- Animal proteins ---- Inactive C-alpha-formylglycine-generating enzyme 2 Source.50: DFBPPR20605 ---- Animal proteins ---- 26S proteasome non-ATPase regulatory subunit 13 Source.51: DFBPPR20623 ---- Animal proteins ---- Retina and anterior neural fold homeobox protein 2 Source.52: DFBPPR21488 ---- Animal proteins ---- Probable ubiquitin carboxyl-terminal hydrolase MINDY-4 Source.53: DFBPPR21508 ---- Animal proteins ---- GPI transamidase component PIG-S Source.54: DFBPPR22555 ---- Animal proteins ---- Bcl-2-like protein 15 Source.55: DFBPPR8554 ---- Animal proteins ---- Glutamate carboxypeptidase 2 Source.56: DFBPPR8566 ---- Animal proteins ---- Scavenger receptor class B member 1 Source.57: DFBPPR8613 ---- Animal proteins ---- Thyroglobulin Source.58: DFBPPR9044 ---- Animal proteins ---- Albumin Source.59: DFBPPR9208 ---- Animal proteins ---- Uteroferrin-associated protein Source.60: DFBPPR9456 ---- Animal proteins ---- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 Source.61: DFBPPR9915 ---- Animal proteins ---- Uteroferrin-associated basic protein 2 Source.62: DFBPPR11153 ---- Animal proteins ---- Toll-interacting protein Source.63: DFBPPR11213 ---- Animal proteins ---- 26S proteasome non-ATPase regulatory subunit 13 Source.64: DFBPPR11548 ---- Animal proteins ---- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 Source.65: DFBPPR11588 ---- Animal proteins ---- Acetylserotonin O-methyltransferase Source.66: DFBPPR11935 ---- Animal proteins ---- Retinal homeobox protein Rx2 Source.67: DFBPPR11956 ---- Animal proteins ---- Retinal homeobox protein Rx1 Source.68: DFBPPR12290 ---- Animal proteins ---- Ryanodine receptor 2 Source.69: DFBPPR12416 ---- Animal proteins ---- Oxysterol-binding protein 1 Source.70: DFBPPR14177 ---- Marine protein ---- Toll-interacting protein Source.71: DFBPPR14487 ---- Marine protein ---- Chloroplast envelope membrane protein Source.72: DFBPPR14668 ---- Marine protein ---- Toll-interacting protein A Source.73: DFBPPR14671 ---- Marine protein ---- Toll-interacting protein B Source.74: DFBPPR15077 ---- Microorganism protein ---- Endopolyphosphatase Source.75: DFBPPR15250 ---- Microorganism protein ---- DNA replication regulator SLD2 Source.76: DFBPPR15457 ---- Microorganism protein ---- Ribosome biogenesis protein RLP24 Source.77: DFBPPR15483 ---- Microorganism protein ---- Elongation factor 2 Source.78: DFBPPR7802 ---- Plant protein ---- Phytochrome B
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

ACE-inhibitory activity	The peptide was potential Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) inhibitory peptide (data not shown). The bioactive sequences were AF, RA.
Specific target protein(s)	Specific Target Protein(s): Angiotensin-converting enzyme

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

N[C@@]([H])(CCC(=O)O)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)N[C@@]([H])(C)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Raw Black bean proteins were hydrolyzed with pepsin/pancreatin.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Mojica L, Chen K, de Mejía EG. Impact of commercial precooking of common bean (Phaseolus vulgaris) on the generation of peptides, after pepsin-pancreatin hydrolysis, capable to inhibit dipeptidyl peptidase-IV. J Food Sci. 2015 Jan;80(1):H188-98. PMID: 25495131 DOI: 10.1111/1750-3841.12726
Other literature(s)	N.D
PubDate	2015