Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPACEI1748(ACE-inhibitory peptide)

DFBP ID	DFBPACEI1748
Peptide sequence	YLLF
Type	Native peptide
Peptide/Function name	ACE-inhibitory peptide

Function-activity relationship

Main bioactivity	ACE-inhibitory activity
Otheir bioactivity	Opioid activity ^[D1], Multifunctional activity ^[D2]

Calculated physicochemical properties

Three-letter amino acid

Tyr-Leu-Leu-Phe

Single-letter amino acid

YLLF

Peptide length

Peptide mass

Experimental mass	Theoretical mass
473 Da	554.67 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

5.92 ^c

IC₅₀

172 uM

pIC₅₀

-2.236

GRAVY

2.2750^c

Hydrophilic residue ratio

75%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal, Marine
Organism/Source	Marine shrimp (Acetes chinensis)
Precursor protein	A. chinensis protein hydrolysates
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR0115 ---- Milk proteins ---- Beta-lactoglobulin Source.2: DFBPPR1115 ---- Plant proteins ---- Jasmonoyl--L-amino acid synthetase GH3.3 Source.3: DFBPPR1175 ---- Plant proteins ---- Gibberellin 3-beta-dioxygenase 2 Source.4: DFBPPR1306 ---- Plant proteins ---- Two pore calcium channel protein 1 Source.5: DFBPPR1384 ---- Plant proteins ---- Oryzalexin E synthase Source.6: DFBPPR1832 ---- Plant proteins ---- ATP-dependent zinc metalloprotease FTSH 7, chloroplastic Source.7: DFBPPR1856 ---- Plant proteins ---- Aminopeptidase M1-D Source.8: DFBPPR1860 ---- Plant proteins ---- Kinesin-like protein KIN-7A Source.9: DFBPPR2171 ---- Plant proteins ---- Probable mixed-linked glucan synthase 8 Source.10: DFBPPR2456 ---- Plant proteins ---- Probable xyloglucan glycosyltransferase 9 Source.11: DFBPPR2535 ---- Plant proteins ---- Probable glucuronosyltransferase Os04g0650300 Source.12: DFBPPR2937 ---- Plant proteins ---- Probable xyloglucan glycosyltransferase 7 Source.13: DFBPPR2969 ---- Plant proteins ---- Probable xyloglucan glycosyltransferase 3 Source.14: DFBPPR3009 ---- Plant proteins ---- Probable xyloglucan glycosyltransferase 2 Source.15: DFBPPR3030 ---- Plant proteins ---- Ammonium transporter 1 member 3 Source.16: DFBPPR3136 ---- Plant proteins ---- Magnesium/proton exchanger 1 Source.17: DFBPPR3210 ---- Plant proteins ---- Ammonium transporter 1 member 2 Source.18: DFBPPR3281 ---- Plant proteins ---- Ammonium transporter 1 member 1 Source.19: DFBPPR3320 ---- Plant proteins ---- Probable xyloglucan glycosyltransferase 1 Source.20: DFBPPR3322 ---- Plant proteins ---- Putative xyloglucan glycosyltransferase 10 Source.21: DFBPPR3367 ---- Plant proteins ---- Probable phytol kinase 1, chloroplastic Source.22: DFBPPR3702 ---- Plant proteins ---- Probable indole-3-acetic acid-amido synthetase GH3.1 Source.23: DFBPPR3707 ---- Plant proteins ---- Probable indole-3-acetic acid-amido synthetase GH3.11 Source.24: DFBPPR3847 ---- Plant proteins ---- Probable indole-3-acetic acid-amido synthetase GH3.13 Source.25: DFBPPR3944 ---- Plant proteins ---- Magnesium/proton exchanger 2 Source.26: DFBPPR4176 ---- Plant proteins ---- 5'-adenylylsulfate reductase-like 3 Source.27: DFBPPR4772 ---- Plant proteins ---- SEC1 family transport protein SLY1 Source.28: DFBPPR5221 ---- Plant proteins ---- Protein TIC 214 Source.29: DFBPPR6148 ---- Plant proteins ---- Uncharacterized protein ycf70 Source.30: DFBPPR6670 ---- Plant proteins ---- Two pore calcium channel protein 1 Source.31: DFBPPR7117 ---- Plant proteins ---- Two pore calcium channel protein 1 Source.32: DFBPPR7652 ---- Milk proteins ---- Zinc transporter 4 Source.33: DFBPPR7687 ---- Milk proteins ---- Beta-lactoglobulin Source.34: DFBPPR7698 ---- Milk proteins ---- Beta-lactoglobulin-1/B Source.35: DFBPPR7714 ---- Milk proteins ---- Beta-lactoglobulin Source.36: DFBPPR8499 ---- Milk proteins ---- Beta-lactoglobulin Source.37: DFBPPR8507 ---- Milk proteins ---- Polycystin-2 Source.38: DFBPPR16197 ---- Animal proteins ---- Cytochrome c oxidase subunit 1 Source.39: DFBPPR16805 ---- Animal proteins ---- Dopamine beta-hydroxylase Source.40: DFBPPR16817 ---- Animal proteins ---- Cytochrome c oxidase subunit 1 Source.41: DFBPPR17366 ---- Animal proteins ---- Beta-adrenergic receptor kinase 1 Source.42: DFBPPR17452 ---- Animal proteins ---- CD81 antigen Source.43: DFBPPR17678 ---- Animal proteins ---- Ubiquitin carboxyl-terminal hydrolase 16 Source.44: DFBPPR17749 ---- Animal proteins ---- CD9 antigen Source.45: DFBPPR17811 ---- Animal proteins ---- YTH domain-containing family protein 2 Source.46: DFBPPR17890 ---- Animal proteins ---- Neuronal acetylcholine receptor subunit alpha-3 Source.47: DFBPPR17921 ---- Animal proteins ---- Ubiquitin carboxyl-terminal hydrolase 2 Source.48: DFBPPR17994 ---- Animal proteins ---- Lysophospholipid acyltransferase 7 Source.49: DFBPPR18000 ---- Animal proteins ---- Very-long-chain enoyl-CoA reductase Source.50: DFBPPR18002 ---- Animal proteins ---- Toll-like receptor 10 Source.51: DFBPPR18452 ---- Animal proteins ---- Ubiquitin carboxyl-terminal hydrolase 22 Source.52: DFBPPR18833 ---- Animal proteins ---- Ubiquitin carboxyl-terminal hydrolase 1 Source.53: DFBPPR18906 ---- Animal proteins ---- NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial Source.54: DFBPPR19730 ---- Animal proteins ---- Tensin-1 Source.55: DFBPPR19899 ---- Animal proteins ---- Receptor expression-enhancing protein 6 Source.56: DFBPPR19908 ---- Animal proteins ---- DNA replication licensing factor MCM6 Source.57: DFBPPR20470 ---- Animal proteins ---- Orexigenic neuropeptide QRFP Source.58: DFBPPR20650 ---- Animal proteins ---- WD repeat-containing protein 91 Source.59: DFBPPR20752 ---- Animal proteins ---- Tetraspanin-33 Source.60: DFBPPR20824 ---- Animal proteins ---- CD151 antigen Source.61: DFBPPR20999 ---- Animal proteins ---- Protein SERAC1 Source.62: DFBPPR21154 ---- Animal proteins ---- Proton-activated chloride channel Source.63: DFBPPR21410 ---- Animal proteins ---- Trans-2,3-enoyl-CoA reductase-like Source.64: DFBPPR22043 ---- Animal proteins ---- Leukocyte antigen CD37 Source.65: DFBPPR22444 ---- Animal proteins ---- Tetraspanin-11 Source.66: DFBPPR9072 ---- Animal proteins ---- CD9 antigen Source.67: DFBPPR9094 ---- Animal proteins ---- Aquaporin-5 Source.68: DFBPPR9703 ---- Animal proteins ---- Membrane-associated transporter protein Source.69: DFBPPR10046 ---- Animal proteins ---- Gallinacin-2 Source.70: DFBPPR10049 ---- Animal proteins ---- Transient receptor potential cation channel subfamily V member 4 Source.71: DFBPPR10224 ---- Animal proteins ---- Prolyl 3-hydroxylase 1 Source.72: DFBPPR10232 ---- Animal proteins ---- Neuronal acetylcholine receptor subunit alpha-4 Source.73: DFBPPR10335 ---- Animal proteins ---- RNA-binding protein with multiple splicing 2 Source.74: DFBPPR10360 ---- Animal proteins ---- Neuronal acetylcholine receptor subunit alpha-3 Source.75: DFBPPR10503 ---- Animal proteins ---- Follicle-stimulating hormone receptor Source.76: DFBPPR10519 ---- Animal proteins ---- Prolyl 3-hydroxylase 2 Source.77: DFBPPR10565 ---- Animal proteins ---- Acetylcholine receptor subunit delta Source.78: DFBPPR10617 ---- Animal proteins ---- Neuronal acetylcholine receptor subunit alpha-6 Source.79: DFBPPR10832 ---- Animal proteins ---- BMP/retinoic acid-inducible neural-specific protein 1 Source.80: DFBPPR10860 ---- Animal proteins ---- Ubiquitin carboxyl-terminal hydrolase 2 Source.81: DFBPPR10989 ---- Animal proteins ---- Neuronal acetylcholine receptor subunit alpha-2 Source.82: DFBPPR11001 ---- Animal proteins ---- Neuronal acetylcholine receptor subunit beta-3 Source.83: DFBPPR11135 ---- Animal proteins ---- Popeye domain-containing protein 3 Source.84: DFBPPR11142 ---- Animal proteins ---- Type I iodothyronine deiodinase Source.85: DFBPPR11212 ---- Animal proteins ---- Glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1 Source.86: DFBPPR11438 ---- Animal proteins ---- NADH-ubiquinone oxidoreductase chain 4 Source.87: DFBPPR11573 ---- Animal proteins ---- tRNA-specific adenosine deaminase 1 Source.88: DFBPPR11698 ---- Animal proteins ---- NADH-cytochrome b5 reductase 2 Source.89: DFBPPR11804 ---- Animal proteins ---- WD repeat-containing protein 91 Source.90: DFBPPR12091 ---- Animal proteins ---- Neurofibromin Source.91: DFBPPR12180 ---- Animal proteins ---- Arylamine N-acetyltransferase, liver isozyme Source.92: DFBPPR12279 ---- Animal proteins ---- Phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform Source.93: DFBPPR12392 ---- Animal proteins ---- Large neutral amino acids transporter small subunit 2 Source.94: DFBPPR12550 ---- Animal proteins ---- Cytochrome c oxidase subunit 1 Source.95: DFBPPR13228 ---- Animal proteins ---- Cytochrome c oxidase subunit 1 Source.96: DFBPPR13444 ---- Animal proteins ---- Cytochrome c oxidase subunit 1 Source.97: DFBPPR13595 ---- Animal proteins ---- Cytochrome c oxidase subunit 1 Source.98: DFBPPR13819 ---- Animal proteins ---- Aquaporin-5 Source.99: DFBPPR15056 ---- Microorganism protein ---- RuvB-like helicase 2 Source.100: DFBPPR15144 ---- Microorganism protein ---- Palmitoyltransferase PFA4 Source.101: DFBPPR15275 ---- Microorganism protein ---- Exocyst complex protein EXO70 Source.102: DFBPPR15498 ---- Microorganism protein ---- Autophagy-related protein 22 Source.103: DFBPPR15662 ---- Microorganism protein ---- Nuclear rim protein 1 Source.104: DFBPPR8120 ---- Plant protein ---- Protein TIC 214 Source.105: DFBPPR8304 ---- Plant protein ---- Protein TIC 214
Link-research Inductive analysis	Link 1: DFBPACEI0019----Milk protein----β-Lactoglobulin

Biological/Functional activity & target protein

ACE-inhibitory activity	The peptide exhibited potent Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) inhibitory activity with an IC₅₀ value of 172 uM.
Specific target protein(s)	Specific Target Protein(s): Angiotensin-converting enzyme

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(Cc1ccc(O)cc1)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Marine A. chinensis was hydrolyzed with a protease produced by Bacillus sp. SM98011.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

The novel peptides from hydrolysate of A. chinensis and some of their derived peptides with high ACE inhibitory activity probably have potential in the treatment of hypertension or in clinical nutrition.

Database cross-references

DFBP

[D1]	DFBPOPIO0003, DFBPOPIO0075
[D2]	DFBPMUFU0010

[D3]

[D4]

[D5]

[D6]

Reference(s)

Primary literature	Hai-Lun H, Xiu-Lan C, Cai-Yun S, Yu-Zhong Z, Bai-Cheng Z. Analysis of novel angiotensin-I-converting enzyme inhibitory peptides from protease-hydrolyzed marine shrimp Acetes chinensis. J Pept Sci. 2006 Nov;12(11):726-33. PMID: 16981241 DOI: 10.1002/psc.789
Other literature(s)	N.D
PubDate	2006