Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPACEI2253(ACE-inhibitory peptide)

DFBP ID	DFBPACEI2253
Peptide sequence	NKGPR
Type	Native peptide
Peptide/Function name	ACE-inhibitory peptide

Function-activity relationship

Main bioactivity	ACE-inhibitory activity
Otheir bioactivity	Antioxidative activity ^[D1], Multifunctional activity ^[D2]

Calculated physicochemical properties

Three-letter amino acid

Asn-Lys-Gly-Pro-Arg

Single-letter amino acid

NKGPR

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	570.65 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

11.54 ^c

IC₅₀

150 uM

pIC₅₀

-2.176

GRAVY

-2.7800^c

Hydrophilic residue ratio

40%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Plant, Food
Organism/Source	Pixian broad bean paste (PBBP)
Precursor protein	The water-soluble peptide extracts of PBBP
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR14393 ---- Marine protein ---- Ribonuclease E/G-like protein
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

ACE-inhibitory activity	The peptide NKGPR exhibited Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) inhibitory activity with an IC₅₀ value of 150 uM.
Specific target protein(s)	Specific Target Protein(s): Angiotensin-converting enzyme

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

N.D

Preparation method

Mode of preparation	Fermentation
Enzyme(s)/starter culture	Pixian broad bean paste employed in this study was provided by Sichuan Pixian Douban Co., Ltd. (Pixian, Sichuan, China) and held at −20 ℃.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

DFBP

[D1]	DFBPANOX1204
[D2]	DFBPMUFU0651

[D3]

[D4]

[D5]

[D6]

Reference(s)

Primary literature	Li, M., Fan, W. and Xu, Y. (2021) Identification of angiotensin converting enzyme (ACE) inhibitory and antioxidant peptides derived from Pixian broad bean paste. LWT, 151, 112221. DOI: 10.1016/j.lwt.2021.112221
Other literature(s)	N.D
PubDate	2021