Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPAGPE0003(Alpha-gliadin peptide)

DFBP ID	DFBPAGPE0003
Peptide sequence	YPQPQPF
Type	Native peptide
Peptide/Function name	α-Gliadin peptide, α-Gliadin-(43-49)

Function-activity relationship

Main bioactivity	Coeliac toxic activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Tyr-Pro-Gln-Pro-Gln-Pro-Phe

Single-letter amino acid

YPQPQPF

Peptide length

Peptide mass

Experimental mass	Theoretical mass
875.9600 Da	875.98 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

5.92 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-1.4714^c

Hydrophilic residue ratio

57.14%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Plant
Organism/Source	Wheat gluten
Precursor protein	α-Gliadin
Residue position	f(43-49)^[1]
Precursor protein(s) search	Source.1: DFBPPR0379 ---- Plant protein ---- Alpha-gliadin Source.2: DFBPPR0380 ---- Plant protein ---- Alpha-gliadin Source.3: DFBPPR0381 ---- Plant protein ---- Alpha-gliadin Source.4: DFBPPR0382 ---- Plant protein ---- Alpha-gliadin Source.5: DFBPPR0383 ---- Plant protein ---- Alpha-gliadin Source.6: DFBPPR0384 ---- Plant protein ---- Alpha-gliadin Source.7: DFBPPR0385 ---- Plant protein ---- Alpha-gliadin Source.8: DFBPPR0389 ---- Plant protein ---- Alpha-gliadin Source.9: DFBPPR0390 ---- Plant protein ---- B3144=ALPHA-gliadin derived CELIAC active peptide Source.10: DFBPPR0391 ---- Plant protein ---- B3143=ALPHA-gliadin derived CELIAC active peptide Source.11: DFBPPR6853 ---- Plant proteins ---- Alpha/beta-gliadin MM1 Source.12: DFBPPR6930 ---- Plant proteins ---- Alpha/beta-gliadin Source.13: DFBPPR6934 ---- Plant proteins ---- Alpha/beta-gliadin clone PW1215 Source.14: DFBPPR6936 ---- Plant proteins ---- Alpha/beta-gliadin A-II Source.15: DFBPPR6937 ---- Plant proteins ---- Alpha/beta-gliadin A-IV Source.16: DFBPPR6938 ---- Plant proteins ---- Alpha/beta-gliadin clone PW8142 Source.17: DFBPPR6939 ---- Plant proteins ---- Alpha/beta-gliadin A-V Source.18: DFBPPR6940 ---- Plant proteins ---- Alpha/beta-gliadin A-III Source.19: DFBPPR6941 ---- Plant proteins ---- Alpha/beta-gliadin A-I
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Coeliac toxic activity

The peptide was tested for inhibition of leukocyte migration in 47 patients with celiac disease. In nineteen patients, all on normal diet, leukocyte migration was inhibited by the peptides and naloxone blocked this effect. In twenty-eight patients (24 of whom were on strict gluten-free diet) leukocyte migration was not affected by the peptides. The results suggest that α-gliadin-(43-49) is closely related to the active fragment, or to one of the active fragments of α-gliadin, and that it interacts with receptors that are similar to but not identical with the known opiate receptors.

Specific target protein(s)

N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(Cc1ccc(O)cc1)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)O

Preparation method

Mode of preparation	Synthesis peptide
Enzyme(s)/starter culture	The peptide was synthesized using solid-phase methodology.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Graf L, Horvath K, Walcz E, Berzetei I, Burnier J. Effect of two synthetic alpha-gliadin peptides on lymphocytes in celiac disease: identification of a novel class of opioid receptors. Neuropeptides. 1987 Feb-Mar;9(2):113-22. PMID: 3033541 DOI: 10.1016/0143-4179(87)90050-3
Other literature(s)	[1] Kasarda D D, Okita T W, Bernardin J E, et al. Nucleic Acid (cDNA) and Amino Acid Sequences of α -Type Gliadins from Wheat (Triticum aestivum)[J]. Proceedings of the National Academy of Sciences of the United States of America, 1984, 81(15):4712-4716.
PubDate	1987