Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPAGPE0004(Alpha-gliadin peptide)

DFBP ID	DFBPAGPE0004
Peptide sequence	QQPYPQ
Type	Native peptide
Peptide/Function name	α-Gliadin peptide, Coeliac toxic peptide

Function-activity relationship

Main bioactivity	Coeliac toxic activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Gln-Gln-Pro-Tyr-Pro-Gln

Single-letter amino acid

QQPYPQ

Peptide length

Peptide mass

Experimental mass	Theoretical mass
759.7970 Da	759.82 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

5.92 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-2.5000^c

Hydrophilic residue ratio

33.33%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Plant
Organism/Source	Wheat gluten
Precursor protein	α-Gliadin
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR0379 ---- Plant protein ---- Alpha-gliadin Source.2: DFBPPR0380 ---- Plant protein ---- Alpha-gliadin Source.3: DFBPPR0381 ---- Plant protein ---- Alpha-gliadin Source.4: DFBPPR0382 ---- Plant protein ---- Alpha-gliadin Source.5: DFBPPR0383 ---- Plant protein ---- Alpha-gliadin Source.6: DFBPPR0384 ---- Plant protein ---- Alpha-gliadin Source.7: DFBPPR0385 ---- Plant protein ---- Alpha-gliadin Source.8: DFBPPR0389 ---- Plant protein ---- Alpha-gliadin Source.9: DFBPPR0390 ---- Plant protein ---- B3144=ALPHA-gliadin derived CELIAC active peptide Source.10: DFBPPR0391 ---- Plant protein ---- B3143=ALPHA-gliadin derived CELIAC active peptide Source.11: DFBPPR6853 ---- Plant proteins ---- Alpha/beta-gliadin MM1 Source.12: DFBPPR6930 ---- Plant proteins ---- Alpha/beta-gliadin Source.13: DFBPPR6934 ---- Plant proteins ---- Alpha/beta-gliadin clone PW1215 Source.14: DFBPPR6936 ---- Plant proteins ---- Alpha/beta-gliadin A-II Source.15: DFBPPR6937 ---- Plant proteins ---- Alpha/beta-gliadin A-IV Source.16: DFBPPR6938 ---- Plant proteins ---- Alpha/beta-gliadin clone PW8142 Source.17: DFBPPR6939 ---- Plant proteins ---- Alpha/beta-gliadin A-V Source.18: DFBPPR6940 ---- Plant proteins ---- Alpha/beta-gliadin A-III Source.19: DFBPPR6941 ---- Plant proteins ---- Alpha/beta-gliadin A-I Source.20: DFBPPR6960 ---- Plant proteins ---- Gamma-gliadin Source.21: DFBPPR6965 ---- Plant proteins ---- Gamma-gliadin B Source.22: DFBPPR7265 ---- Plant proteins ---- Gamma-hordein-3
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Coeliac toxic activity	The peptide QQPYPQ was toxicity to patients with coeliac disease. The tests showed that digestion products from patients coeliac mucoas caused damage to rat liver lysosomes in contrast to digestion products from normal mucosa which had practically no effects on the lysosomes (Mean activity was 37%, means of two results, expressed as % reduction in absorbance compared with zero-time tubes).
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	No prediction can be made about the peptide bitterness. ^prediction

SMILES

N[C@@]([H])(CCC(=O)N)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	The peptide was obtained by peptic-tryptic-pancreatinic digest of wheat gliadin.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Cornell HJ. Amino acid composition of peptides remaining after in vitro digestion of a gliadin sub-fraction with duodenal mucosa from patients with coeliac disease. Clin Chim Acta. 1988 Sep 15;176(3):279-89. PMID: 3180479 DOI: 10.1016/0009-8981(88)90187-8
Other literature(s)	N.D
PubDate	1988