Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPAGPE0010(Alpha-gliadin peptide)

DFBP ID	DFBPAGPE0010
Peptide sequence	LGQQQPFPPQQPYPQPQPF
Type	Native peptide
Peptide/Function name	α-Gliadin peptide, Coeliac toxic peptide

Function-activity relationship

Main bioactivity	Coeliac toxic activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Leu-Gly-Gln-Gln-Gln-Pro-Phe-Pro-Pro-Gln-Gln-Pro-Tyr-Pro-Gln-Pro-Gln-Pro-Phe

Single-letter amino acid

LGQQQPFPPQQPYPQPQPF

Peptide length

Peptide mass

Experimental mass	Theoretical mass
2222.4719 Da	2222.50 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

5.92 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-1.4737^c

Hydrophilic residue ratio

57.89%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Plant
Organism/Source	Wheat gluten
Precursor protein	α-Gliadin
Residue position	f(31-49)
Precursor protein(s) search	Source.1: DFBPPR0379 ---- Plant protein ---- Alpha-gliadin Source.2: DFBPPR0380 ---- Plant protein ---- Alpha-gliadin Source.3: DFBPPR0381 ---- Plant protein ---- Alpha-gliadin Source.4: DFBPPR0384 ---- Plant protein ---- Alpha-gliadin Source.5: DFBPPR0389 ---- Plant protein ---- Alpha-gliadin Source.6: DFBPPR6930 ---- Plant proteins ---- Alpha/beta-gliadin Source.7: DFBPPR6941 ---- Plant proteins ---- Alpha/beta-gliadin A-I
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Coeliac toxic activity	The peptide LGQQQPFPPQQPYPQPQPF is toxic in vivo. Since this oligopeptide is recognised by HLADQ2-restricted T cells, the observed effects may be due to immune activation within the intestinal mucosa.
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(CC(C)C)C(=O)NCC(=O)N[C@@]([H])(CCC(=O)N)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)N1[C@@]([H])(CCC1)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)O

Preparation method

Mode of preparation	Synthesis peptide
Enzyme(s)/starter culture	The oligopeptide was synthesised with a solid-phase peptide synthesiser.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Sturgess R, Day P, Ellis HJ, Lundin KE, Gjertsen HA, Kontakou M, Ciclitira PJ. Wheat peptide challenge in coeliac disease. Lancet. 1994 Mar 26;343(8900):758-61. PMID: 7907731 DOI: 10.1016/s0140-6736(94)91837-6
Other literature(s)	N.D
PubDate	1994