Sera of patients with coeliac disease, containing IgA and IgG
antigliadin antibodies (AGA) and various IgA autoantibodies , react with
isolated enterocytes. Reactivity of gliadin with all purified AGA tested was inhibited by peptide A4 (QEQVPLVQQQQF) at the N-terminal region of the gliadin molecule. AGA cross reactivity with enteroctyes and calreticulin was also inhibited by peptide A4. As dominant epitopes AGA of coeliac patients recognise similar structures corresponding to peptides A4 present on gliadin, enterocytes, and calreticulin. Substitution of glutamine in the A4 peptide by glutamic acid caused loss of inhibitory capacity. Shortening of peptide A4 on the N-terminal by three amino acids increased its inhibitory effect. |