Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPAGPE0066(Alpha-gliadin peptide)

DFBP ID	DFBPAGPE0066
Peptide sequence	PFPPQQPYPQPQPF
Type	Native peptide
Peptide/Function name	α-Gliadin peptide, Potential coeliac toxic peptide (extended motif)

Function-activity relationship

Main bioactivity	Alpha-gliadin peptide activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Pro-Phe-Pro-Pro-Gln-Gln-Pro-Tyr-Pro-Gln-Pro-Gln-Pro-Phe

Single-letter amino acid

PFPPQQPYPQPQPF

Peptide length

Peptide mass

Experimental mass	Theoretical mass
1667.8670 Da	1667.88 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

5.92 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-1.4929^c

Hydrophilic residue ratio

64.29%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Plant
Organism/Source	Wheat
Precursor protein	α-Gliadin
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR0379 ---- Plant protein ---- Alpha-gliadin Source.2: DFBPPR0380 ---- Plant protein ---- Alpha-gliadin Source.3: DFBPPR0381 ---- Plant protein ---- Alpha-gliadin Source.4: DFBPPR0383 ---- Plant protein ---- Alpha-gliadin Source.5: DFBPPR0384 ---- Plant protein ---- Alpha-gliadin Source.6: DFBPPR0389 ---- Plant protein ---- Alpha-gliadin Source.7: DFBPPR0390 ---- Plant protein ---- B3144=ALPHA-gliadin derived CELIAC active peptide Source.8: DFBPPR6853 ---- Plant proteins ---- Alpha/beta-gliadin MM1 Source.9: DFBPPR6930 ---- Plant proteins ---- Alpha/beta-gliadin Source.10: DFBPPR6936 ---- Plant proteins ---- Alpha/beta-gliadin A-II Source.11: DFBPPR6941 ---- Plant proteins ---- Alpha/beta-gliadin A-I
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Coeliac toxic activity	Extended motifs were defined as the peptide sequences containing the toxic motifs. They were extended by means of including neighbouring amino acid residues selected from the alpha-gliadin sequence. Celiac toxic
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)N1[C@@]([H])(CCC1)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)O

Preparation method

Mode of preparation	null
Enzyme(s)/starter culture	null

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

Coeliac disease is a permanent intolerance to the gluten or storage proteins found in some cereals. According to De Ritis and coworkers, small sequences of amino acids are common to the peptides linked with the toxicity. It has also been suggested that Tyr-containing motif is also associated with toxicity.
Peptide derived from apha-gliadin.

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	McLachlan A, Cullis PG, Cornell HJ. The use of extended amino acid motifs for focussing on toxic peptides in coeliac disease. J Biochem Mol Biol Biophys. 2002 Oct;6(5):319-24. PMID: 12385967 DOI: 10.1080/1025814021000003238
Other literature(s)	N.D
PubDate	2002