Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPAGPE0251(Alpha-gliadin peptide)

DFBP ID	DFBPAGPE0251
Peptide sequence	VPVPQLQPQNPSQQQPQEQ
Type	Native peptide
Peptide/Function name	α-Gliadin peptide, Coeliac toxic peptide

Function-activity relationship

Main bioactivity	Coeliac toxic activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Val-Pro-Val-Pro-Gln-Leu-Gln-Pro-Gln-Asn-Pro-Ser-Gln-Gln-Gln-Pro-Gln-Glu-Gln

Single-letter amino acid

VPVPQLQPQNPSQQQPQEQ

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	2170.39 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

3.34 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-1.6632^c

Hydrophilic residue ratio

42.11%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Plant
Organism/Source	Wheat gluten
Precursor protein	α-Gliadin
Residue position	f(3-21)
Precursor protein(s) search	Source.1: DFBPPR0379 ---- Plant protein ---- Alpha-gliadin Source.2: DFBPPR0380 ---- Plant protein ---- Alpha-gliadin Source.3: DFBPPR0381 ---- Plant protein ---- Alpha-gliadin Source.4: DFBPPR0382 ---- Plant protein ---- Alpha-gliadin Source.5: DFBPPR0385 ---- Plant protein ---- Alpha-gliadin Source.6: DFBPPR0389 ---- Plant protein ---- Alpha-gliadin Source.7: DFBPPR0390 ---- Plant protein ---- B3144=ALPHA-gliadin derived CELIAC active peptide Source.8: DFBPPR0391 ---- Plant protein ---- B3143=ALPHA-gliadin derived CELIAC active peptide Source.9: DFBPPR6853 ---- Plant proteins ---- Alpha/beta-gliadin MM1 Source.10: DFBPPR6939 ---- Plant proteins ---- Alpha/beta-gliadin A-V Source.11: DFBPPR6940 ---- Plant proteins ---- Alpha/beta-gliadin A-III Source.12: DFBPPR6941 ---- Plant proteins ---- Alpha/beta-gliadin A-I
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Coeliac toxic activity	Although the C-terminal peptide VPVPQLQPQNPSQQQPQEQ is toxic in vivo, this peptide did not cause histological changes in any of the patients.
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

N[C@@]([H])(C(C)C)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(C(C)C)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)N[C@@]([H])(CC(=O)N)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CO)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)N[C@@]([H])(CCC(=O)O)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)O

Preparation method

Mode of preparation	Synthesis peptide
Enzyme(s)/starter culture	The oligopeptide was synthesised with a solid-phase peptide synthesiser.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Sturgess R, Day P, Ellis HJ, Lundin KE, Gjertsen HA, Kontakou M, Ciclitira PJ. Wheat peptide challenge in coeliac disease. Lancet. 1994 Mar 26;343(8900):758-61. PMID: 7907731 DOI: 10.1016/s0140-6736(94)91837-6
Other literature(s)	N.D
PubDate	1994