Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPALAM0005(α-Amylase inhibitory peptide)

DFBP ID	DFBPALAM0005
Peptide sequence	EVSGL
Type	Native peptide
Peptide/Function name	α-amylase inhibitory peptide, Anti-diabetic peptide

Function-activity relationship

Main bioactivity	Alpha-amylase inhibitory activity
Otheir bioactivity	α-Glucosidase inhibitory activity ^[D1], Multifunctional activity ^[D2]

Calculated physicochemical properties

Three-letter amino acid

Glu-Val-Ser-Gly-Leu

Single-letter amino acid

EVSGL

Peptide length

Peptide mass

Experimental mass	Theoretical mass
503.3752 Da	503.55 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

3.34 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

0.6600^c

Hydrophilic residue ratio

60%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Egg White protein
Precursor protein	Albumin
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR1260 ---- Plant proteins ---- Peptide deformylase 1A, chloroplastic Source.2: DFBPPR1261 ---- Plant proteins ---- Auxin efflux carrier component 3a Source.3: DFBPPR1709 ---- Plant proteins ---- Beta-fructofuranosidase, insoluble isoenzyme 1 Source.4: DFBPPR2024 ---- Plant proteins ---- Methylthioribose-1-phosphate isomerase Source.5: DFBPPR3266 ---- Plant proteins ---- Protein MOR1 Source.6: DFBPPR16748 ---- Animal proteins ---- Thyroid adenoma-associated protein homolog Source.7: DFBPPR20394 ---- Animal proteins ---- Actin filament-associated protein 1-like 2 Source.8: DFBPPR22460 ---- Animal proteins ---- Coiled-coil domain-containing protein 149 Source.9: DFBPPR9993 ---- Animal proteins ---- Ovalbumin Source.10: DFBPPR10950 ---- Animal proteins ---- Ovalbumin-related protein Y Source.11: DFBPPR11242 ---- Animal proteins ---- GDNF family receptor alpha-1 Source.12: DFBPPR11636 ---- Animal proteins ---- Epithelial splicing regulatory protein 2 Source.13: DFBPPR11951 ---- Animal proteins ---- Integrator complex subunit 2
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

α-Amylase inhibitory activity	Inhibitor of alpha-amylase (EC 3.2.1.1). Peptide EVSGL did not show significant inhibitory effects on α-glucosidase (with an IC₅₀ of > 150.0 umol/l) and α-amylase (with an IC₅₀ of > 150.0 umol/l).
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

N[C@@]([H])(CCC(=O)O)C(=O)N[C@@]([H])(C(C)C)C(=O)N[C@@]([H])(CO)C(=O)NCC(=O)N[C@@]([H])(CC(C)C)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	The alkaline proteinase Alcalase was added at 4.0% of protein (w/w) for digestion.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

DFBP

[D1]	DFBPALGL0012
[D2]	DFBPMUFU0744

[D3]

[D4]

[D5]

[D6]

Reference(s)

Primary literature	Yu Z, Yin Y, Zhao W, Liu J, Chen F. Anti-diabetic activity peptides from albumin against α-glucosidase and α-amylase. Food Chem. 2012 Dec 1;135(3):2078-85. PMID: 22953959 DOI: 10.1016/j.foodchem.2012.06.088
Other literature(s)	[1] Yu Z, Yin Y, Zhao W, et al. Novel peptides derived from egg white protein inhibiting alpha-glucosidase[J]. Food Chemistry, 2011, 129(4):1376-1382.
PubDate	2012