DFBP ID - DFBPALAM0018(α-Amylase inhibitory peptide)

DFBP ID DFBPALAM0018 Peptide sequence HAEIN Type Native peptide Peptide/Function name α-amylase inhibitory peptide, Anti-diabetic peptide

Function-activity relationship

Main bioactivity Alpha-amylase inhibitory activity Otheir bioactivity Antioxidative activity [D1], Antithrombotic activity [D2], α-Glucosidase inhibitory activity [D3], Multifunctional activity [D4]

Calculated physicochemical properties

Three-letter amino acid His-Ala-Glu-Ile-Asn Single-letter amino acid HAEIN Peptide length 5 Peptide mass Experimental mass Theoretical mass N.D 582.61 Da c Net charge 0.00 c Isoelectric point (pI) 5.17 c IC50 N.D pIC50 N.D GRAVY -0.7800 c Hydrophilic residue ratio 40% c Peptide calculator To calculate the physicochemical properties of bioactive peptide.

Peptide source & Food-borne protein(s) search

Classification Animal Organism/Source Egg white protein Precursor protein Ovalbumin (386AA) Residue position f(332-336) Precursor protein(s) search Source.1: DFBPPR9993 ---- Animal proteins ---- Ovalbumin Link-research Inductive analysis There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

α-Amylase inhibitory activity Inhibitor of alpha-amylase (EC 3.2.1.1). The peptide did not exhibit visible or detectable inhibitory effect on the α-glucosidase (IC50 > 150.0 umol/L) and α-amylase (IC50 > 150.0 umol/L). Specific target protein(s) N.D

Taste properties & Structure

Bitterness Literature report N.D Bitter prediction tools Non-bitter taste prediction SMILES N[C@@]([H])(CC1=CN=C-N1)C(=O)N[C@@]([H])(C)C(=O)N[C@@]([H])(CCC(=O)O)C(=O)N[C@@]([H])([C@]([H])(CC)C)C(=O)N[C@@]([H])(CC(=O)N)C(=O)O

Preparation method

Mode of preparation Enzymatic hydrolysis Enzyme(s)/starter culture The alkaline proteinase Alcalase was added to initiate the protein enzymatic hydrolysis.

Stability & Cytotoxicity

Peptide stability Literature report: N.D EHP-Tool: Enzymatic Hydrolysis Prediction Tool (EHP-Tool) Peptide cytotoxicity Literature report: N.D Prediction: ToxinPred

Additional information

Additional information N.D

Database cross-references

DFBP [D1] DFBPANOX0626 [D2] DFBPANTH0090 [D3] DFBPALGL0009 [D4] DFBPMUFU0676

BIOPEP-UWM [D5] 9395

APD [D6] -

BioPepDB [D7] -

MBPDB [D8] -

Reference(s)

Primary literature Yu, Z., Yin, Y., Zhao, W., Yu, Y., Liu, B., Liu, J., et al. Novel peptides derived from egg white protein inhibiting alpha-glucosidase. Food Chem. 2011, 129, 1376-82. DOI: 10.1016/j.foodchem.2011.05.067 Other literature(s) N.D PubDate 2011