Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPAMIC0001(Antimicrobial peptide)

DFBP ID	DFBPAMIC0001
Peptide sequence	NLCERASLTWTGNCGNTGHCDTQCRNWESAKHGACHKRGNWKCFCYFNC
Type	Native peptide
Peptide/Function name	Antimicrobial peptide, Ct-AMP1

Function-activity relationship

Main bioactivity	Antimicrobial activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Asn-Leu-Cys-Glu-Arg-Ala-Ser-Leu-Thr-Trp-Thr-Gly-Asn-Cys-Gly-Asn-Thr-Gly-His-Cys-Asp-Thr-Gln-Cys-Arg-Asn-Trp-Glu-Ser-Ala-Lys-His-Gly-Ala-Cys-His-Lys-Arg-Gly-Asn-Trp-Lys-Cys-Phe-Cys-Tyr-Phe-Asn-Cys

Single-letter amino acid

NLCERASLTWTGNCGNTGHCDTQCRNWESAKHGACHKRGNWKCFCYFNC

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	5613.31 Da ^c

Net charge

Isoelectric point (pI)

8.07 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-0.8490^c

Hydrophilic residue ratio

30.61%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Plant
Organism/Source	Clitoria ternatea
Precursor protein	Extraction of seed proteins(Seeds were purchased from Chiltern Seeds (Cumbria, UK) or collected locally (A. hippocastanum))
Residue position	N.D
Precursor protein(s) search	No matching precursor protein found
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antimicrobial activity

Antifungal and antibacterial assays were carried out as literature described ^[1,2]. In medium A supplemented with 1 mM CaCl₂ and 50 mM KCl, the antifungal activity of Ct-AMP1 as follows:

	IC₅₀ (ug/ml)
Botrytis cinerea	20
Cladosporium sphaerospermum	6
Fusarium cubnorum	10
Leptosphaeria maculans	6
Penicillium digitatum	20
Trichoderma viride	> 100
Septoria tritiei	2
Verticilium alboatrum	2

Specific target protein(s)

N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

N[C@@]([H])(CC(=O)N)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(CS)C(=O)N[C@@]([H])(CCC(=O)O)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)N[C@@]([H])(C)C(=O)N[C@@]([H])(CO)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])([C@]([H])(O)C)C(=O)N[C@@]([H])(CC(=CN2)C1=C2C=CC=C1)C(=O)N[C@@]([H])([C@]([H])(O)C)C(=O)NCC(=O)N[C@@]([H])(CC(=O)N)C(=O)N[C@@]([H])(CS)C(=O)NCC(=O)N[C@@]([H])(CC(=O)N)C(=O)N[C@@]([H])([C@]([H])(O)C)C(=O)NCC(=O)N[C@@]([H])(CC1=CN=C-N1)C(=O)N[C@@]([H])(CS)C(=O)N[C@@]([H])(CC(=O)O)C(=O)N[C@@]([H])([C@]([H])(O)C)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)N[C@@]([H])(CS)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)N[C@@]([H])(CC(=O)N)C(=O)N[C@@]([H])(CC(=CN2)C1=C2C=CC=C1)C(=O)N[C@@]([H])(CCC(=O)O)C(=O)N[C@@]([H])(CO)C(=O)N[C@@]([H])(C)C(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(CC1=CN=C-N1)C(=O)NCC(=O)N[C@@]([H])(C)C(=O)N[C@@]([H])(CS)C(=O)N[C@@]([H])(CC1=CN=C-N1)C(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)NCC(=O)N[C@@]([H])(CC(=O)N)C(=O)N[C@@]([H])(CC(=CN2)C1=C2C=CC=C1)C(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(CS)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)N[C@@]([H])(CS)C(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)N[C@@]([H])(CC(=O)N)C(=O)N[C@@]([H])(CS)C(=O)O

Preparation method

Mode of preparation	Extraction of seed proteins without enzyme
Enzyme(s)/starter culture	No enzyme

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Osborn RW, De Samblanx GW, Thevissen K, Goderis I, Torrekens S, Van Leuven F, Attenborough S, Rees SB, Broekaert WF. Isolation and characterisation of plant defensins from seeds of Asteraceae, Fabaceae, Hippocastanaceae and Saxifragaceae. FEBS Lett. 1995 Jul 17;368(2):257-62. PMID: 7628617 DOI: 10.1016/0014-5793(95)00666-w
Other literature(s)	[1] Terras F R, Schoofs H M, De Bolle M F, et al. Analysis of two novel classes of plant antifungal proteins from radish (Raphanus sativus L.) seeds[J]. Journal of Biological Chemistry, 1992, 267(22):15301-9. [2] Broekaert, Willem F, Terras, Franky R.G, Cammue, Bruno P.A, et al. An automated quantitative assay for fungal growth inhibition[J]. FEMS Microbiology Letters, 1990, 69(1‐2):55-59.
PubDate	1995