DFBP ID - DFBPAMIC0008(Antimicrobial peptide)

DFBP ID DFBPAMIC0008 Peptide sequence QRVRNPQSCRWNMGVCIPFLCRVGMRQIGTCFGPRVPCCRR Type Native peptide Peptide/Function name Antimicrobial peptide, Bovine Beta-defensin 4

Function-activity relationship

Main bioactivity Antimicrobial activity Otheir bioactivity N.D

Calculated physicochemical properties

Three-letter amino acid Gln-Arg-Val-Arg-Asn-Pro-Gln-Ser-Cys-Arg-Trp-Asn-Met-Gly-Val-Cys-Ile-Pro-Phe-Leu-Cys-Arg-Val-Gly-Met-Arg-Gln-Ile-Gly-Thr-Cys-Phe-Gly-Pro-Arg-Val-Pro-Cys-Cys-Arg-Arg Single-letter amino acid QRVRNPQSCRWNMGVCIPFLCRVGMRQIGTCFGPRVPCCRR Peptide length 41 Peptide mass Experimental mass Theoretical mass N.D 4782.79 Da c Net charge +8 Isoelectric point (pI) 11.66 c IC50 N.D pIC50 N.D GRAVY -0.2415 c Hydrophilic residue ratio 48.78% c Peptide calculator To calculate the physicochemical properties of bioactive peptide.

Peptide source & Food-borne protein(s) search

Classification Animal Organism/Source Bos taurus (Bovine) Precursor protein Neutrophil protein Residue position N.D Precursor protein(s) search Source.1: DFBPPR18526 ---- Animal proteins ---- Beta-defensin 4 Link-research Inductive analysis There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antimicrobial activity The peptide showed effective bactericidal activity against E.coli ML35 and S.aureus 502A. Specific target protein(s) N.D

Taste properties & Structure

Bitterness Literature report N.D Bitter prediction tools Non-bitter taste prediction SMILES N[C@@]([H])(CCC(=O)N)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)N[C@@]([H])(C(C)C)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)N[C@@]([H])(CC(=O)N)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)N[C@@]([H])(CO)C(=O)N[C@@]([H])(CS)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)N[C@@]([H])(CC(=CN2)C1=C2C=CC=C1)C(=O)N[C@@]([H])(CC(=O)N)C(=O)N[C@@]([H])(CCSC)C(=O)NCC(=O)N[C@@]([H])(C(C)C)C(=O)N[C@@]([H])(CS)C(=O)N[C@@]([H])([C@]([H])(CC)C)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(CS)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)N[C@@]([H])(C(C)C)C(=O)NCC(=O)N[C@@]([H])(CCSC)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)N[C@@]([H])([C@]([H])(CC)C)C(=O)NCC(=O)N[C@@]([H])([C@]([H])(O)C)C(=O)N[C@@]([H])(CS)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)NCC(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)N[C@@]([H])(C(C)C)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CS)C(=O)N[C@@]([H])(CS)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)O

Preparation method

Mode of preparation Synthesis Enzyme(s)/starter culture No enzyme

Stability & Cytotoxicity

Peptide stability Literature report: N.D EHP-Tool: Enzymatic Hydrolysis Prediction Tool (EHP-Tool) Peptide cytotoxicity Literature report: N.D Prediction: ToxinPred

Additional information

Additional information N.D

Database cross-references

BIOPEP-UWM [D1] 5506

APD [D2] -

BioPepDB [D3] -

MBPDB [D4] -

Reference(s)

Primary literature Selsted ME, Tang YQ, Morris WL, McGuire PA, Novotny MJ, Smith W, Henschen AH, Cullor JS. Purification, primary structures, and antibacterial activities of beta-defensins, a new family of antimicrobial peptides from bovine neutrophils. J Biol Chem. 1993 Mar 25;268(9):6641-8. PMID: 8454635 Other literature(s) N.D PubDate 1993