Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPAMIC0084(Antimicrobial peptide)

DFBP ID	DFBPAMIC0084
Peptide sequence	RIIDLLWRVRRPQKPKFVTVWVR
Type	Native peptide
Peptide/Function name	Antimicrobial peptide, PMAP-23

Function-activity relationship

Main bioactivity	Antimicrobial activity
Otheir bioactivity	Anticancer activity ^[D1]

Calculated physicochemical properties

Three-letter amino acid

Arg-Ile-Ile-Asp-Leu-Leu-Trp-Arg-Val-Arg-Arg-Pro-Gln-Lys-Pro-Lys-Phe-Val-Thr-Val-Trp-Val-Arg

Single-letter amino acid

RIIDLLWRVRRPQKPKFVTVWVR

Peptide length

Peptide mass

Experimental mass	Theoretical mass
2962.50 Da	2962.60 Da ^c

Net charge

Isoelectric point (pI)

12.68 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-0.2957^c

Hydrophilic residue ratio

56.52%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Pig (Sus scrofa)
Precursor protein	Myeloid cells
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR9568 ---- Animal proteins ---- Antibacterial peptide PMAP-23
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antimicrobial activity

(1) anti-Gram+ & Gram-, antifungal;
(2) Active against E. coli ML35, E. coli ATCC 25922, S. typhimurium ATCC 14028, P. aeruginosa ATCC 27853 (MIC 16 uM), B. megaterium (local isolate), and S. aureus ATCC 25923 (MIC 2-8 uM, except for P. aeruginosa), as shown in table 1:

Table 1 Antibacterial activity of PMAP-23
Organism and strain	MIC (uM)
E. coli ML35	2
E. coli ATCC 25922	4
S. typhimurium ATCC 14028	8
P. aeruginosa ATCC 27853	16
B. megaterium (local isolate)	2
S. aureus ATCC 25923	4

(3) Also antifungal against C. albicans, S. cerevisiae and T. beigelii (MIC 2-5 uM) ^[1].

Specific target protein(s)

N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(CCCNC(=N)N)C(=O)N[C@@]([H])([C@]([H])(CC)C)C(=O)N[C@@]([H])([C@]([H])(CC)C)C(=O)N[C@@]([H])(CC(=O)O)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(CC(=CN2)C1=C2C=CC=C1)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)N[C@@]([H])(C(C)C)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)N[C@@]([H])(CCCCN)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)N[C@@]([H])(C(C)C)C(=O)N[C@@]([H])([C@]([H])(O)C)C(=O)N[C@@]([H])(C(C)C)C(=O)N[C@@]([H])(CC(=CN2)C1=C2C=CC=C1)C(=O)N[C@@]([H])(C(C)C)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)O

Preparation method

Mode of preparation	Extract without enzyme
Enzyme(s)/starter culture	No enzyme, total RNA was extracted from pig bone marrow cells with guanidinium thiocyanate.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

DFBP

[D1]	DFBPANCA0867

[D2]

[D3]

[D4]

[D5]

Reference(s)

Primary literature	Zanetti M, Storici P, Tossi A, Scocchi M, Gennaro R. Molecular cloning and chemical synthesis of a novel antibacterial peptide derived from pig myeloid cells. J Biol Chem. 1994 Mar 18;269(11):7855-8. PMID: 8132502
Other literature(s)	[1] Lee D G, Kim D H, Park Y, et al. Fungicidal effect of antimicrobial peptide, PMAP-23, isolated from porcine myeloid against Candida albicans[J]. Biochemical & Biophysical Research Communications, 2001, 282(2):570-4. [2] Gennaro R, Skerlavaj B, Romeo D. Purification, composition, and activity of two bactenecins, antibacterial peptides of bovine neutrophils[J]. Infection & Immunity, 1989, 57(10):3142.
PubDate	1994