Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPAMIC0145(Antimicrobial peptide)

DFBP ID	DFBPAMIC0145
Peptide sequence	AFPPPNVPGPRFPPPNFPGPRFPPPNFPGPRFPPPNFPGPRFPPPNFPGPPFPPPIFPGPWFPPPPPFRPPPFGPPRFP
Type	Native peptide
Peptide/Function name	Antimicrobial peptide, Prophenin-1

Function-activity relationship

Main bioactivity	Antimicrobial activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Ala-Phe-Pro-Pro-Pro-Asn-Val-Pro-Gly-Pro-Arg-Phe-Pro-Pro-Pro-Asn-Phe-Pro-Gly-Pro-Arg-Phe-Pro-Pro-Pro-Asn-Phe-Pro-Gly-Pro-Arg-Phe-Pro-Pro-Pro-Asn-Phe-Pro-Gly-Pro-Arg-Phe-Pro-Pro-Pro-Asn-Phe-Pro-Gly-Pro-Pro-Phe-Pro-Pro-Pro-Ile-Phe-Pro-Gly-Pro-Trp-Phe-Pro-Pro-Pro-Pro-Pro-Phe-Arg-Pro-Pro-Pro-Phe-Gly-Pro-Pro-Arg-Phe-Pro

Single-letter amino acid

AFPPPNVPGPRFPPPNFPGPRFPPPNFPGPRFPPPNFPGPRFPPPNFPGPPFPPPIFPGPWFPPPPPFRPPPFGPPRFP

Peptide length

Peptide mass

Experimental mass	Theoretical mass
8683.00 Da	8681.05 Da ^c

Net charge

Isoelectric point (pI)

13.21 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-0.7962^c

Hydrophilic residue ratio

86.08%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Sus scrofa (Pig)
Precursor protein	Porcine leukocytes
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR9113 ---- Animal proteins ---- Prophenin and tritrpticin precursor
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antimicrobial activity	(1) anti-Gram+ & Gram-; (2) Prophenin-1 was encoded on a cathelin-containing precursor and showed substantially more activity against E. coli, a Gram-negative bacterium, than against Listeria monocytogenes, a Gram-positive organism, in vitro.
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

N.D

Preparation method

Mode of preparation	Acid extraction without enzyme
Enzyme(s)/starter culture	No enzyme

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Harwig SS, Kokryakov VN, Swiderek KM, Aleshina GM, Zhao C, Lehrer RI. Prophenin-1, an exceptionally proline-rich antimicrobial peptide from porcine leukocytes. FEBS Lett. 1995 Mar 27;362(1):65-9. PMID: 7698355 DOI: 10.1016/0014-5793(95)00210-z
Other literature(s)	N.D
PubDate	1995