(1) The effective active sequence of the peptide is GRRKRKWLRRIGKGVKIIGGAALDHL-NH2;
(2) anti-Gram+ & Gram-, antifungal
(3) Highly active against A. salmonicida 99-1, A. salmonicida 97-4, S.
enterica serovar Typhimurium MS7953s, S. enterica serovar Typhimurium
14028s, P. aeruginosa K799, P. aeruginosa Z61, E. coli CGSC4908, E. coli
UB1005, E. coli DC2, S. epidermidis C621, MRSA C623, C. albicans C627
(MIC 1-8 uM). Table 1. Bactericidal activities of selected antimicrobial peptidesa
Peptide code
| Concn
(fold MIC)
| Pathogen
| Maximal killing (% bacteria killed)
| Time when maximal killing was reached
| NRC-1
| 1
10
1
10
1
10
| 99-1
99-1
MS7953s
MS7953s
C. albicans
C. albicans
| 100
100
100
100
NK
90
| 40
20
20
10
NA
240
| NRC-3
| 1
10
1
10
| 99-1
99-1
MS7953s
MS7953s
| 90
100
97
100
| 20
5
60
20
| NRC-12
| 1
10
| C. albicans
C. albicans | NK
90
| NA
240
| NRC-13
| 1
10
| K799
K799
| 95
100
| 10
5
| NRC-13 with NaCl
| 1
10
| K799
K799
| 5
100
| 60
20
| NRC-13
| 1
10
1
10
| MRSA
MRSA
C. albicans
C. albicans
| 99
100
25
75
| 10
5
240
240
| NRC-15
| 1
10
1
10
| K799
K799
MRSA
MRSA
| 60
98
100
100
| 60
10
20
5
| NRC-16
| 1
10
| C. albicans
C. albicans | 35
92
| 240
240
| NRC-17
| 1
10
| C. albicans
C. albicans | 10
95
| 240
240
| NRC-19
| 1
10
1
10
| MRSA
MRSA
K799
K799
| 33
90
70
80
| 60
10
60
40
| a The bacterial strains used are A. salmonicida (99-1), S. typhimurium (MS7953s), P. aeruginosa (K799), and MRSA. NK, no killing; NA, not available.
| |
