Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPAMIC0186(Antimicrobial peptide)

DFBP ID	DFBPAMIC0186
Peptide sequence	LKKISQ
Type	Native peptide
Peptide/Function name	Antimicrobial peptide

Function-activity relationship

Main bioactivity	Antimicrobial activity
Otheir bioactivity	ACE-inhibitory activity ^[D1], Antioxidative activity ^[D2], Multifunctional activity ^[D3]

Calculated physicochemical properties

Three-letter amino acid

Leu-Lys-Lys-Ile-Ser-Gln

Single-letter amino acid

LKKISQ

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	715.88 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

10.59 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-0.6333^c

Hydrophilic residue ratio

33.33%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Ovine milk
Precursor protein	α_s2-Casein
Residue position	f(165-170)
Precursor protein(s) search	Source.1: DFBPPR7689 ---- Milk proteins ---- Alpha-S2-casein Source.2: DFBPPR7701 ---- Milk proteins ---- Alpha-S2-casein Source.3: DFBPPR8494 ---- Milk proteins ---- Alpha-S2-casein
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antimicrobial activity	The peptide showed effective bactericidal activity against M.flavus DSM 1790, L. innocua, B. cereus P7, St. Thermophilus Rs, E. Coli ATCC 25922, E. Coli MC 1061.
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	No prediction can be made about the peptide bitterness. ^prediction

SMILES

N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])([C@]([H])(CC)C)C(=O)N[C@@]([H])(CO)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Hydrolysis with Pepsin.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

DFBP

[D1]	DFBPACEI1800
[D2]	DFBPANOX0716
[D3]	DFBPMUFU0537

[D4]

[D5]

[D6]

[D7]

Reference(s)

Primary literature	López-Expósito, I., Gómez-Ruiz, J.Á., Amigo, L., Recio, I. Identification of antibacterial peptides from ovine αs2-casein. International Dairy Journal. 2006, 16, 1072-80. DOI: 10.1016/j.idairyj.2005.10.006
Other literature(s)	[1] Park Y W. Bioactive Components in Milk and Dairy Products[M]. 2009. [2] López-Expósito I, Quirós A, Amigo L, et al. Casein hydrolysates as a source of antimicrobial, antioxidant and antihypertensive peptides[J]. Dairy Science & Technology, 2007, 87(4):241-249.
PubDate	2006