DFBP ID - DFBPAMIC0341(Antimicrobial peptide)

DFBP ID DFBPAMIC0341 Peptide sequence GLFDVIKKVASVIGGLG Type Native peptide Peptide/Function name Antimicrobial peptide

Function-activity relationship

Main bioactivity Antimicrobial activity Otheir bioactivity Anticancer activity [D1], Multifunctional activity [D2]

Calculated physicochemical properties

Three-letter amino acid Gly-Leu-Phe-Asp-Val-Ile-Lys-Lys-Val-Ala-Ser-Val-Ile-Gly-Gly-Leu-Gly Single-letter amino acid GLFDVIKKVASVIGGLG Peptide length 17 Peptide mass Experimental mass Theoretical mass N.D 1673.02 Da c Net charge 0.00 c Isoelectric point (pI) 9.84 c IC50 N.D pIC50 N.D GRAVY 1.1824 c Hydrophilic residue ratio 76.47% c Peptide calculator To calculate the physicochemical properties of bioactive peptide.

Peptide source & Food-borne protein(s) search

Classification Animal Organism/Source Australian Blue Mountains tree frog Litoria citropa Precursor protein Skin gland protein Residue position N.D Precursor protein(s) search No matching precursor protein found Link-research Inductive analysis There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antimicrobial activity The peptide showed bactericidal activity against L. lactis, M. luteus and S. epidermidis. Specific target protein(s) N.D

Taste properties & Structure

Bitterness Literature report N.D Bitter prediction tools No prediction can be made about the peptide bitterness. prediction SMILES NCC(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)N[C@@]([H])(CC(=O)O)C(=O)N[C@@]([H])(C(C)C)C(=O)N[C@@]([H])([C@]([H])(CC)C)C(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(C(C)C)C(=O)N[C@@]([H])(C)C(=O)N[C@@]([H])(CO)C(=O)N[C@@]([H])(C(C)C)C(=O)N[C@@]([H])([C@]([H])(CC)C)C(=O)NCC(=O)NCC(=O)N[C@@]([H])(CC(C)C)C(=O)NCC(=O)O

Preparation method

Mode of preparation Synthesis Enzyme(s)/starter culture No enzyme

Stability & Cytotoxicity

Peptide stability Literature report: N.D EHP-Tool: Enzymatic Hydrolysis Prediction Tool (EHP-Tool) Peptide cytotoxicity Literature report: N.D Prediction: ToxinPred

Additional information

Additional information N.D

Database cross-references

DFBP [D1] DFBPANCA0068 [D2] DFBPMUFU0711

BIOPEP-UWM [D3] 3911, 3916, 4016, 7857

APD [D4] -

BioPepDB [D5] -

MBPDB [D6] -

Reference(s)

Primary literature Wabnitz, P.; Bowie, J.; Wallace, J.; Tyler, M., The citropin peptides from the skin glands of the Australian Blue Mountains tree frog Litoria citropa. Part 2: Sequence determination using electrospray mass spectrometry. Rapid communications in mass spectrometry : RCM 1999,13, 1724-1732. DOI: 10.1002/(SICI)1097-0231(19990915)13:17 Other literature(s) N.D PubDate 1999