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List of peptide properties
DFBP ID - DFBPAMIC0523(Antimicrobial peptide)
Peptide sequence ISAEAFGAINEPMK
Type Native peptide
Peptide/Function name Antimicrobial peptide, Tg-Hb P4
Function-activity relationship
Main bioactivity Antimicrobial activity
Otheir bioactivity N.D
Calculated physicochemical properties
Three-letter amino acid Ile-Ser-Ala-Glu-Ala-Phe-Gly-Ala-Ile-Asn-Glu-Pro-Met-Lys
Single-letter amino acid ISAEAFGAINEPMK
Peptide length 14
Peptide mass
Experimental mass Theoretical mass
1480 Da 1477.67 Da c
Net charge 0.00 c
Isoelectric point (pI) 4.53
IC50 N.D
pIC50 N.D
GRAVY 0.1357 c
Hydrophilic residue ratio 57.14% c
Peptide calculator
To calculate the physicochemical properties of bioactive peptide.
Peptide source & Food-borne protein(s) search
Classification Animal, Marine, Bivalve molluscs
Organism/Source Blood clam (Tegillarca granosa)
Precursor protein Hemoglobin
Residue position N.D
Precursor protein(s) search
No matching precursor protein found
There are no literature reports on the discovery of this sequence in other food-source proteins.
Biological/Functional activity & target protein
Antimicrobial activity

As shown in Table 3, the minimal inhibitory concentration (MIC) of Tg-Hb P4 for V. alginolyticus, V. parahaemolyticus and V. harveyi was 12 μg/mL, 12 μg/mL and 24 μg/mL, respectively.

Specific target protein(s) N.D
Taste properties & Structure
Literature report N.D
Bitter prediction tools Non-bitter taste prediction
Preparation method
Mode of preparation

Enzymatic hydrolysis

Enzyme(s)/starter culture

An aliquot of purified Tegillarca granosa hemoglobin was mixed with trypsin (Sigma, USA) to a final protease: sample ratio of 1:50 (w/w). After incubation at 37 ℃ for 2 h, ice-cold trichloroacetic acid (TCA) was added into the mixture to a final concentration of 10% to precipitate proteins and released peptides.

Stability & Cytotoxicity
Peptide stability
Literature report: N.D
EHP-Tool: Enzymatic Hydrolysis Prediction Tool (EHP-Tool)
Peptide cytotoxicity
Literature report: N.D
Prediction: ToxinPred
Additional information
Additional information N.D
Database cross-references
APD [D2] -
BioPepDB [D3] -
MBPDB [D4] -
Primary literature Bao Y, Wang J, Li C, Li P, Wang S, Lin Z. A preliminary study on the antibacterial mechanism of Tegillarca granosa hemoglobin by derived peptides and peroxidase activity. Fish Shellfish Immunol. 2016 Apr;51:9-16.
PMID: 26876330
Other literature(s) N.D
PubDate 2016
Copyright © 2020. Record / license number: Chongqing ICP No. 2000214