Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPAMIC0524(Antimicrobial peptide)

DFBP ID	DFBPAMIC0524
Peptide sequence	GHAITLTYALNNFVDSLDDPSR
Type	Native peptide
Peptide/Function name	Antimicrobial peptide, Tg-Hb P5

Function-activity relationship

Main bioactivity	Antimicrobial activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Gly-His-Ala-Ile-Thr-Leu-Thr-Tyr-Ala-Leu-Asn-Asn-Phe-Val-Asp-Ser-Leu-Asp-Asp-Pro-Ser-Arg

Single-letter amino acid

GHAITLTYALNNFVDSLDDPSR

Peptide length

Peptide mass

Experimental mass	Theoretical mass
2420 Da	2419.59 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

4.41

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-0.2273^c

Hydrophilic residue ratio

45.45%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal, Marine, Bivalve molluscs
Organism/Source	Blood clam (Tegillarca granosa)
Precursor protein	Hemoglobin
Residue position	N.D
Precursor protein(s) search	No matching precursor protein found
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antimicrobial activity	As shown in Table 3, the minimal inhibitory concentration (MIC) of Tg-Hb P5 for V. alginolyticus, V. parahaemolyticus and V. harveyi was 200 μg/mL, 100 μg/mL and 200 μg/mL, respectively.
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

N.D

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	An aliquot of purified Tegillarca granosa hemoglobin was mixed with trypsin (Sigma, USA) to a final protease: sample ratio of 1:50 (w/w). After incubation at 37 ℃ for 2 h, ice-cold trichloroacetic acid (TCA) was added into the mixture to a final concentration of 10% to precipitate proteins and released peptides.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Bao Y, Wang J, Li C, Li P, Wang S, Lin Z. A preliminary study on the antibacterial mechanism of Tegillarca granosa hemoglobin by derived peptides and peroxidase activity. Fish Shellfish Immunol. 2016 Apr;51:9-16. PMID: 26876330 DOI: 10.1016/j.fsi.2016.02.004
Other literature(s)	N.D
PubDate	2016