Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPAMIC0527(Antimicrobial peptide)

DFBP ID	DFBPAMIC0527
Peptide sequence	QKKCPGRCTLKCGKHERPTLPYNCGKYICCVPVKVK
Type	Native peptide
Peptide/Function name	Antimicrobial peptide

Function-activity relationship

Main bioactivity	Antimicrobial activity
Otheir bioactivity	Antiviral activity ^[D1], Multifunctional activity ^[D2]

Calculated physicochemical properties

Three-letter amino acid

Gln-Lys-Lys-Cys-Pro-Gly-Arg-Cys-Thr-Leu-Lys-Cys-Gly-Lys-His-Glu-Arg-Pro-Thr-Leu-Pro-Tyr-Asn-Cys-Gly-Lys-Tyr-Ile-Cys-Cys-Val-Pro-Val-Lys-Val-Lys

Single-letter amino acid

QKKCPGRCTLKCGKHERPTLPYNCGKYICCVPVKVK

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	4080.03 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

9.73 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-0.6083^c

Hydrophilic residue ratio

36.11%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal, Marine
Organism/Source	Marine turtle (Caretta caretta)
Precursor protein	Egg white protein
Residue position	N.D
Precursor protein(s) search	No matching precursor protein found
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antimicrobial activity	This peptide showed strong antibacterial activity against Escherichia coli and Salmonella typhimurium.
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N.D

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	The protein was sequenced by a combination of sequential Edman degradation, carboxypeptidase digestion, nuclear magnetic resonance and electrospray ionization tandem mass spectrometry.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

This small cationic protein is part of the innate immunity of this organism and replaces lysozyme in the egg. It has the potential to be developed as an antibacterial and antiviral agent.

Database cross-references

DFBP

[D1]	DFBPANPE0013
[D2]	DFBPMUFU0717

[D3]

[D4]

[D5]

[D6]

Reference(s)

Primary literature	Chattopadhyay S, Sinha NK, Banerjee S, Roy D, Chattopadhyay D, Roy S. Small cationic protein from a marine turtle has beta-defensin-like fold and antibacterial and antiviral activity. Proteins. 2006 Aug 1;64(2):524-31. PMID: 16700051 DOI: 10.1002/prot.20963
Other literature(s)	N.D
PubDate	2006