Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANCA0028(Anticancer peptide)

DFBP ID	DFBPANCA0028
Peptide sequence	GFKDLLKGAAKALVKTVLF
Type	Native peptide
Peptide/Function name	Anticancer peptide, Ascaphin-8

Function-activity relationship

Main bioactivity	Anticancer activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Gly-Phe-Lys-Asp-Leu-Leu-Lys-Gly-Ala-Ala-Lys-Ala-Leu-Val-Lys-Thr-Val-Leu-Phe

Single-letter amino acid

GFKDLLKGAAKALVKTVLF

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	2019.48 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

10.55 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

0.7368^c

Hydrophilic residue ratio

68.42%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal, Amphibian
Organism/Source	Ascaphidae, Tailed frog (Ascaphus truei)
Precursor protein	Skin secretions
Residue position	N.D
Precursor protein(s) search	No matching precursor protein found
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Anticancer activity	Cytotoxicity on HepG2 cells.
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

NCC(=O)N[C@@]([H])(Cc1ccccc1)C(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(CC(=O)O)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(CCCCN)C(=O)NCC(=O)N[C@@]([H])(C)C(=O)N[C@@]([H])(C)C(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(C)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(C(C)C)C(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])([C@]([H])(O)C)C(=O)N[C@@]([H])(C(C)C)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)O

Preparation method

Mode of preparation	Synthesis
Enzyme(s)/starter culture	Synthesis peptide

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Conlon JM, Galadari S, Raza H, Condamine E. Design of potent, non-toxic antimicrobial agents based upon the naturally occurring frog skin peptides, ascaphin-8 and peptide XT-7. Chem Biol Drug Des. 2008 Jul;72(1):58-64. PMID: 18554256 DOI: 10.1111/j.1747-0285.2008.00671.x
Other literature(s)	[1] Wang L, Dong C, Li X, et al. Anticancer potential of bioactive peptides from animal sources (Review).[J]. Oncology Reports, 2017, 38(2).
PubDate	2008