Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANCA0037(Anticancer peptide)

DFBP ID	DFBPANCA0037
Peptide sequence	MAFLKKSLFLVLFGLVSLSIC
Type	Native peptide
Peptide/Function name	Anticancer peptide, Phylloseptin-PBa

Function-activity relationship

Main bioactivity	Anticancer activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Met-Ala-Phe-Leu-Lys-Lys-Ser-Leu-Phe-Leu-Val-Leu-Phe-Gly-Leu-Val-Ser-Leu-Ser-Ile-Cys

Single-letter amino acid

MAFLKKSLFLVLFGLVSLSIC

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	2329.94 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

9.86 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

1.8905^c

Hydrophilic residue ratio

71.43%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal, Amphibian
Organism/Source	Hylidae, Peruvian purple-sided leaf frog (Phyllomedusa baltea)
Precursor protein	Skin secretions
Residue position	N.D
Precursor protein(s) search	No matching precursor protein found
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Anticancer activity	Peptide MAFLKKSLFLVLF(FL)GLVSLSIC showed anti-proliferative activity against H460 cells, PC3 cells and tU251MG cells.
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(CCSC)C(=O)N[C@@]([H])(C)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(CO)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(C(C)C)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)NCC(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(C(C)C)C(=O)N[C@@]([H])(CO)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(CO)C(=O)N[C@@]([H])([C@]([H])(CC)C)C(=O)N[C@@]([H])(CS)C(=O)O

Preparation method

Mode of preparation	Synthesis
Enzyme(s)/starter culture	Synthesis peptide

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Wan Y, Ma C, Zhou M, Xi X, Li L, Wu D, Wang L, Lin C, Lopez JC, Chen T, Shaw C. Phylloseptin-PBa--A Novel Broad-Spectrum Antimicrobial Peptide from the Skin Secretion of the Peruvian Purple-Sided Leaf Frog (Phyllomedusa Baltea) Which Exhibits Cancer Cell Cytotoxicity. Toxins (Basel). 2015 Dec 1;7(12):5182-93. PMID: 26633506 DOI: 10.3390/toxins7124878
Other literature(s)	[1] Wang L, Dong C, Li X, et al. Anticancer potential of bioactive peptides from animal sources (Review).[J]. Oncology Reports, 2017, 38(2).
PubDate	2015