Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANCA0038(Anticancer peptide)

DFBP ID	DFBPANCA0038
Peptide sequence	GLLDTLKGAAKNVVGSLASKVMEKL
Type	Native peptide
Peptide/Function name	Anticancer peptide, Pentadactylin

Function-activity relationship

Main bioactivity	Anticancer activity
Otheir bioactivity	Antimicrobial activity ^[D1], Multifunctional activity ^[D2]

Calculated physicochemical properties

Three-letter amino acid

Gly-Leu-Leu-Asp-Thr-Leu-Lys-Gly-Ala-Ala-Lys-Asn-Val-Val-Gly-Ser-Leu-Ala-Ser-Lys-Val-Met-Glu-Lys-Leu

Single-letter amino acid

GLLDTLKGAAKNVVGSLASKVMEKL

Peptide length

Peptide mass

Experimental mass	Theoretical mass
2540.5 Da	2543.03 Da ^c

Net charge

Isoelectric point (pI)

10.16 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

0.3720^c

Hydrophilic residue ratio

60%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal, Amphibian
Organism/Source	Leptodactylidae, Pepper frog (Leptodactylus labyrinthicus)
Precursor protein	Skin secretions
Residue position	N.D
Precursor protein(s) search	No matching precursor protein found
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Anticancer activity	Cytotoxic activity on B16F10 cells without high specificity.
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

NCC(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(CC(=O)O)C(=O)N[C@@]([H])([C@]([H])(O)C)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(CCCCN)C(=O)NCC(=O)N[C@@]([H])(C)C(=O)N[C@@]([H])(C)C(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(CC(=O)N)C(=O)N[C@@]([H])(C(C)C)C(=O)N[C@@]([H])(C(C)C)C(=O)NCC(=O)N[C@@]([H])(CO)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(C)C(=O)N[C@@]([H])(CO)C(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(C(C)C)C(=O)N[C@@]([H])(CCSC)C(=O)N[C@@]([H])(CCC(=O)O)C(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(CC(C)C)C(=O)O

Preparation method

Mode of preparation	Synthesis
Enzyme(s)/starter culture	Synthesis peptide

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

DFBP

[D1]	DFBPAMIC0127
[D2]	DFBPMUFU0701

[D3]

[D4]

[D5]

[D6]

Reference(s)

Primary literature	Libério MS, Joanitti GA, Azevedo RB, Cilli EM, Zanotta LC, Nascimento AC, Sousa MV, Pires Júnior OR, Fontes W, Castro MS. Anti-proliferative and cytotoxic activity of pentadactylin isolated from Leptodactylus labyrinthicus on melanoma cells. Amino Acids. 2011 Jan;40(1):51-9. PMID: 20012549 DOI: 10.1007/s00726-009-0384-y
Other literature(s)	[1] Wang L, Dong C, Li X, et al. Anticancer potential of bioactive peptides from animal sources (Review).[J]. Oncology Reports, 2017, 38(2). [2] King J D, Al-Ghaferi N, Abraham B, et al. Pentadactylin: An antimicrobial peptide from the skin secretions of the South American bullfrog Leptodactylus pentadactylus[J]. Comparative Biochemistry & Physiology Part C Toxicology & Pharmacology, 2005, 141(4):393-397.
PubDate	2011