Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANCA0039(Anticancer peptide)

DFBP ID	DFBPANCA0039
Peptide sequence	KLSPETKDNLKKVLKGAIKGAIVAKMV
Type	Native peptide
Peptide/Function name	Anticancer peptide, Hymenochirin-1B

Function-activity relationship

Main bioactivity	Anticancer activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Lys-Leu-Ser-Pro-Glu-Thr-Lys-Asp-Asn-Leu-Lys-Lys-Val-Leu-Lys-Gly-Ala-Ile-Lys-Gly-Ala-Ile-Val-Ala-Lys-Met-Val

Single-letter amino acid

KLSPETKDNLKKVLKGAIKGAIVAKMV

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	2880.54 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

10.66 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-0.0519^c

Hydrophilic residue ratio

55.56%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal, Amphibian
Organism/Source	Pipidae, Congo dwarf clawed frog (Hymenochirus boettgeri)
Precursor protein	Skin secretions
Residue position	N.D
Precursor protein(s) search	No matching precursor protein found
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Anticancer activity	Cytotoxic activity against A549 cells, MDA-MB-231 cells, HT-29 cells, and HepG2 cells.
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	No prediction can be made about the peptide bitterness. ^prediction

SMILES

N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(CO)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CCC(=O)O)C(=O)N[C@@]([H])([C@]([H])(O)C)C(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(CC(=O)O)C(=O)N[C@@]([H])(CC(=O)N)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(C(C)C)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(CCCCN)C(=O)NCC(=O)N[C@@]([H])(C)C(=O)N[C@@]([H])([C@]([H])(CC)C)C(=O)N[C@@]([H])(CCCCN)C(=O)NCC(=O)N[C@@]([H])(C)C(=O)N[C@@]([H])([C@]([H])(CC)C)C(=O)N[C@@]([H])(C(C)C)C(=O)N[C@@]([H])(C)C(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(CCSC)C(=O)N[C@@]([H])(C(C)C)C(=O)O

Preparation method

Mode of preparation	Synthesis
Enzyme(s)/starter culture	Synthesis peptide

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Attoub S, Arafat H, Mechkarska M, Conlon JM. Anti-tumor activities of the host-defense peptide hymenochirin-1B. Regul Pept. 2013 Nov 10;187:51-6. PMID: 24185042 DOI: 10.1016/j.regpep.2013.10.006
Other literature(s)	[1] Wang L, Dong C, Li X, et al. Anticancer potential of bioactive peptides from animal sources (Review).[J]. Oncology Reports, 2017, 38(2).
PubDate	2013