Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANCA0055(Anticancer peptide)

DFBP ID	DFBPANCA0055
Peptide sequence	FKCRRWQWRMKKLGAPSITCVRRAF
Type	Native peptide
Peptide/Function name	Anticancer peptide, Lactoferricin B (Lfcin-B)

Function-activity relationship

Main bioactivity	Anticancer activity
Otheir bioactivity	Antimicrobial activity ^[D1], Immunomodulatory activity ^[D2], Multifunctional activity ^[D3]

Calculated physicochemical properties

Three-letter amino acid

Phe-Lys-Cys-Arg-Arg-Trp-Gln-Trp-Arg-Met-Lys-Lys-Leu-Gly-Ala-Pro-Ser-Ile-Thr-Cys-Val-Arg-Arg-Ala-Phe

Single-letter amino acid

FKCRRWQWRMKKLGAPSITCVRRAF

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	3125.80 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

12.34 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-0.5760^c

Hydrophilic residue ratio

48%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Bovine milk
Precursor protein	Lactoferrin (LF-B)
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR8500 ---- Milk proteins ---- Lactotransferrin
Link-research Inductive analysis	Link 1: DFBPANCA0007----Bovine milk----Lactoferricin (LfcinB) peptide from bovine lactoferrin Link 2: DFBPANCA0334----Bovine milk protein----Lactoferricin

Biological/Functional activity & target protein

Anticancer activity

Lfcin-B is a novel peptide to induce apoptosis in THP-1 human leukemia cells, and that its apoptosis-inducing activity is mediated by the pathway related to production of the intracellular ROS and activation of Ca²⁺/Mg²⁺-dependent endonucleases.

Tumor target	Mechanism
Hematological tumor cells/Solid tumor cells	Membrane permeabilization/Apoptosis

Specific target protein(s)

N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(Cc1ccccc1)C(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(CS)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)N[C@@]([H])(CC(=CN2)C1=C2C=CC=C1)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)N[C@@]([H])(CC(=CN2)C1=C2C=CC=C1)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)N[C@@]([H])(CCSC)C(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(CC(C)C)C(=O)NCC(=O)N[C@@]([H])(C)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CO)C(=O)N[C@@]([H])([C@]([H])(CC)C)C(=O)N[C@@]([H])([C@]([H])(O)C)C(=O)N[C@@]([H])(CS)C(=O)N[C@@]([H])(C(C)C)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)N[C@@]([H])(C)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	The peptide produced by gastric pepsin digestion of bovine lactoferrin^[2].

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

Lfcin-B to induce apoptosis in tumor cells may help to prevent breast-fed host from neoplasia.

Database cross-references

DFBP

[D1]	DFBPAMIC0005
[D2]	DFBPIMMU0026
[D3]	DFBPMUFU0698

BIOPEP-UWM

[D4]

3229, 7051, 8175

APD

[D5]

BioPepDB

[D6]

MBPDB

[D7]

Reference(s)

Primary literature	Yoo YC, Watanabe R, Koike Y, Mitobe M, Shimazaki K, Watanabe S, Azuma I. Apoptosis in human leukemic cells induced by lactoferricin, a bovine milk protein-derived peptide: involvement of reactive oxygen species. Biochem Biophys Res Commun. 1997 Aug 28;237(3):624-8. PMID: 9299415 DOI: 10.1006/bbrc.1997.7199
Other literature(s)	[1] Deslouches B, Di Y P. Antimicrobial peptides with selective antitumor mechanisms: prospect for anticancer applications:[J]. Oncotarget, 2017, 8(28):46635-46651. [2] Bellamy W, Takase M, Wakabayashi H, et al. Antibacterial spectrum of lactoferricin B, a potent bactericidal peptide derived from the N-terminal region of bovine lactoferrin.[J]. Journal of Applied Bacteriology, 1992, 73(6):472-9.
PubDate	1997