Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANCA0061(Anticancer peptide)

DFBP ID	DFBPANCA0061
Peptide sequence	GFFALIPKIISSPLFKTLLSAVGSALSSSGGQE
Type	Native peptide
Peptide/Function name	Anticancer peptide, Pardaxin

Function-activity relationship

Main bioactivity	Anticancer activity
Otheir bioactivity	Antimicrobial activity ^[D1], Multifunctional activity ^[D2]

Calculated physicochemical properties

Three-letter amino acid

Gly-Phe-Phe-Ala-Leu-Ile-Pro-Lys-Ile-Ile-Ser-Ser-Pro-Leu-Phe-Lys-Thr-Leu-Leu-Ser-Ala-Val-Gly-Ser-Ala-Leu-Ser-Ser-Ser-Gly-Gly-Gln-Glu

Single-letter amino acid

GFFALIPKIISSPLFKTLLSAVGSALSSSGGQE

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	3323.81 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

9.84 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

0.7455^c

Hydrophilic residue ratio

63.64%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal, Marine, Fish
Organism/Source	Pardachirus marmoratus
Precursor protein	Red Sea moses sole^[2]
Residue position	N.D
Precursor protein(s) search	No matching precursor protein found
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Anticancer activity

Pardaxin reduced cell viability in a dose-dependent manner. The expression of activated caspase-3 in SCC-4 cells significantly increased after 24-h treatment with pardaxin. Pardaxin treatment resulted in the cell cycle arrest of SCC-4 cells in the G2/M phase, thereby limiting cell proliferation. Furthermore, pardaxin treatment substantially alleviated carcinogenesis in the DMBA-induced hamster buccal pouch model by lowering prostaglandin E2 levels.

Tumor target	Mechanism
Solid tumor cells	Membrane permeabilization

Specific target protein(s)

N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

NCC(=O)N[C@@]([H])(Cc1ccccc1)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)N[C@@]([H])(C)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])([C@]([H])(CC)C)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])([C@]([H])(CC)C)C(=O)N[C@@]([H])([C@]([H])(CC)C)C(=O)N[C@@]([H])(CO)C(=O)N[C@@]([H])(CO)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])([C@]([H])(O)C)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(CO)C(=O)N[C@@]([H])(C)C(=O)N[C@@]([H])(C(C)C)C(=O)NCC(=O)N[C@@]([H])(CO)C(=O)N[C@@]([H])(C)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(CO)C(=O)N[C@@]([H])(CO)C(=O)N[C@@]([H])(CO)C(=O)NCC(=O)NCC(=O)N[C@@]([H])(CCC(=O)N)C(=O)N[C@@]([H])(CCC(=O)O)C(=O)O

Preparation method

Mode of preparation	Synthesis
Enzyme(s)/starter culture	No enzyme, Pardaxin was synthesized by the solid-phase method^[2].

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

Pardaxin is a potential marine drug for adjuvant chemotherapy for human OSCC and oral cancer.

Database cross-references

DFBP

[D1]	DFBPAMIC0136
[D2]	DFBPMUFU0702

[D3]

[D4]

[D5]

[D6]

Reference(s)

Primary literature	Han Y, Cui Z, Li YH, Hsu WH, Lee BH. In Vitro and in Vivo Anticancer Activity of Pardaxin against Proliferation and Growth of Oral Squamous Cell Carcinoma. Mar Drugs. 2015 Dec 23;14(1):2. PMID: 26703631 DOI: 10.3390/md14010002
Other literature(s)	[1] Deslouches B, Di Y P. Antimicrobial peptides with selective antitumor mechanisms: prospect for anticancer applications:[J]. Oncotarget, 2017, 8(28):46635-46651. [2] Shai Y, Fox J, Caratsch C, et al. Sequencing and synthesis of pardaxin, a polypeptide from the Red Sea Moses sole with ionophore activity[J]. Febs Letters, 1988, 242(1):161-166.
PubDate	2015