Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANCA0098(Anticancer peptide)

DFBP ID	DFBPANCA0098
Peptide sequence	GPAGDY
Type	Native peptide
Peptide/Function name	Anticancer peptide

Function-activity relationship

Main bioactivity	Anticancer activity
Otheir bioactivity	Antioxidative activity ^[D1], Multifunctional activity ^[D2]

Calculated physicochemical properties

Three-letter amino acid

Gly-Pro-Ala-Gly-Asp-Tyr

Single-letter amino acid

GPAGDY

Peptide length

Peptide mass

Experimental mass	Theoretical mass
578.58 Da	578.57 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

3.13 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-0.9000^c

Hydrophilic residue ratio

66.67%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal, Fish
Organism/Source	Skate (Raja porosa)
Precursor protein	Cartilage protein
Residue position	N.D
Precursor protein(s) search	No matching precursor protein found
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Anticancer activity	Exhibited anti-proliferation activity by inducing apoptosis. The peptide GPAGDY showed cytotoxicity against HeLa cell lines, with an IC₅₀ of 4.86 mg/ml.
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

NCC(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(C)C(=O)NCC(=O)N[C@@]([H])(CC(=O)O)C(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Skate cartilage protein was hydrolyzed with alcalase and trypsin.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

The peptide GPAGDY from skate cartilage protein hydrolysate may have applications as functional foods and nutraceuticals for the treatment and prevention of cancer.

Database cross-references

DFBP

[D1]	DFBPANOX0595
[D2]	DFBPMUFU0690

[D3]

[D4]

[D5]

[D6]

Reference(s)

Primary literature	Pan X, Zhao YQ, Hu FY, Chi CF, Wang B. Anticancer Activity of a Hexapeptide from Skate (Raja porosa) Cartilage Protein Hydrolysate in HeLa Cells. Mar Drugs. 2016 Aug 16;14(8):153. PMID: 27537897 DOI: 10.3390/md14080153
Other literature(s)	[1] Chalamaiah M, Yu W, Wu J. Immunomodulatory and anticancer protein hydrolysates (peptides) from food proteins: A review.[J]. Food Chemistry, 2018, 245:205-222. [2] Pan X, Zhao Y Q, Hu F Y, et al. Preparation and identification of antioxidant peptides from protein hydrolysate of skate ( Raja porosa ) cartilage[J]. Journal of Functional Foods, 2016, 25:220-230.
PubDate	2016