Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANCA0103(Anticancer peptide)

DFBP ID	DFBPANCA0103
Peptide sequence	REDV
Type	Native peptide
Peptide/Function name	Anticancer peptide

Function-activity relationship

Main bioactivity	Anticancer activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Arg-Glu-Asp-Val

Single-letter amino acid

REDV

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	517.53 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

4.12 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-1.8250^c

Hydrophilic residue ratio

25%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Fibronectin
Precursor protein	The type III connecting segment domain (IIICS) of fibronectin
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR1134 ---- Plant proteins ---- Ethylene-responsive transcription factor FZP Source.2: DFBPPR1182 ---- Plant proteins ---- Calcium-dependent protein kinase 3 Source.3: DFBPPR1316 ---- Plant proteins ---- Tryptophan aminotransferase-related protein 2 Source.4: DFBPPR1321 ---- Plant proteins ---- Calcium-dependent protein kinase 16 Source.5: DFBPPR1503 ---- Plant proteins ---- Porphobilinogen deaminase, chloroplastic Source.6: DFBPPR1623 ---- Plant proteins ---- Probable 4-hydroxy-tetrahydrodipicolinate reductase 2, chloroplastic Source.7: DFBPPR1749 ---- Plant proteins ---- Probable L-ascorbate peroxidase 6, chloroplastic/mitochondrial Source.8: DFBPPR1778 ---- Plant proteins ---- Mitogen-activated protein kinase 11 Source.9: DFBPPR1797 ---- Plant proteins ---- Probable L-ascorbate peroxidase 5, chloroplastic Source.10: DFBPPR1917 ---- Plant proteins ---- Kinesin-like protein KIN-12A Source.11: DFBPPR1963 ---- Plant proteins ---- Potassium channel AKT1 Source.12: DFBPPR1964 ---- Plant proteins ---- Heat stress transcription factor A-5 Source.13: DFBPPR1969 ---- Plant proteins ---- BTB/POZ domain and ankyrin repeat-containing protein NPR3 Source.14: DFBPPR2107 ---- Plant proteins ---- Probable sucrose-phosphate synthase 1 Source.15: DFBPPR2490 ---- Plant proteins ---- Probable glycerol-3-phosphate dehydrogenase [NAD(+)] 2, cytosolic Source.16: DFBPPR2560 ---- Plant proteins ---- Probable glycerol-3-phosphate dehydrogenase [NAD(+)] 3, cytosolic Source.17: DFBPPR2579 ---- Plant proteins ---- Probable glycerol-3-phosphate dehydrogenase [NAD(+)] 1, cytosolic Source.18: DFBPPR2632 ---- Plant proteins ---- Probable 4-coumarate--CoA ligase 4 Source.19: DFBPPR2706 ---- Plant proteins ---- Kinesin-like protein KIN-14H Source.20: DFBPPR2828 ---- Plant proteins ---- ABC transporter G family member 36 Source.21: DFBPPR2870 ---- Plant proteins ---- Homeobox-leucine zipper protein HOX27 Source.22: DFBPPR2909 ---- Plant proteins ---- Probable isoprenylcysteine alpha-carbonyl methylesterase ICME Source.23: DFBPPR3158 ---- Plant proteins ---- Probable adenylate kinase 1, chloroplastic Source.24: DFBPPR3244 ---- Plant proteins ---- Kinesin-like protein KIN-12B Source.25: DFBPPR3266 ---- Plant proteins ---- Protein MOR1 Source.26: DFBPPR3774 ---- Plant proteins ---- Kinesin-like protein KIN-14A Source.27: DFBPPR3786 ---- Plant proteins ---- Kinesin-like protein KIN-14D Source.28: DFBPPR4431 ---- Plant proteins ---- Spindle and kinetochore-associated protein 1 homolog Source.29: DFBPPR4935 ---- Plant proteins ---- UPF0014 membrane protein STAR2 Source.30: DFBPPR5209 ---- Plant proteins ---- Elongation factor G-2, chloroplastic Source.31: DFBPPR5457 ---- Plant proteins ---- Protein PLASTID TRANSCRIPTIONALLY ACTIVE 12, chloroplastic Source.32: DFBPPR5797 ---- Plant proteins ---- DNA-directed RNA polymerase subunit beta Source.33: DFBPPR5803 ---- Plant proteins ---- Anthocyanidin 3-O-glucosyltransferase Source.34: DFBPPR5810 ---- Plant proteins ---- Anthocyanidin 3-O-glucosyltransferase Source.35: DFBPPR6211 ---- Plant proteins ---- Anthocyanidin 3-O-glucosyltransferase Source.36: DFBPPR6304 ---- Plant proteins ---- Porphobilinogen deaminase, chloroplastic Source.37: DFBPPR6347 ---- Plant proteins ---- Granule-bound starch synthase 2, chloroplastic/amyloplastic Source.38: DFBPPR6763 ---- Plant proteins ---- Starch synthase 1, chloroplastic/amyloplastic Source.39: DFBPPR7600 ---- Milk proteins ---- UDP-glucuronosyltransferase 1A1 Source.40: DFBPPR15994 ---- Animal proteins ---- Inactive pancreatic lipase-related protein 1 Source.41: DFBPPR16443 ---- Animal proteins ---- Ubiquitin carboxyl-terminal hydrolase 11 Source.42: DFBPPR16575 ---- Animal proteins ---- Dystrophin Source.43: DFBPPR17130 ---- Animal proteins ---- Glycine receptor subunit alpha-1 Source.44: DFBPPR17163 ---- Animal proteins ---- Coxsackievirus and adenovirus receptor homolog Source.45: DFBPPR17345 ---- Animal proteins ---- Palmitoyl-protein thioesterase 1 Source.46: DFBPPR17375 ---- Animal proteins ---- Peptidyl-glycine alpha-amidating monooxygenase Source.47: DFBPPR17394 ---- Animal proteins ---- Diacylglycerol kinase alpha Source.48: DFBPPR18424 ---- Animal proteins ---- Inositol 1,4,5-trisphosphate receptor type 2 Source.49: DFBPPR18467 ---- Animal proteins ---- Alpha-aminoadipic semialdehyde synthase, mitochondrial Source.50: DFBPPR18719 ---- Animal proteins ---- A-kinase anchor protein 5 Source.51: DFBPPR18787 ---- Animal proteins ---- Rho-related GTP-binding protein RhoU Source.52: DFBPPR18790 ---- Animal proteins ---- Proto-oncogene vav Source.53: DFBPPR19259 ---- Animal proteins ---- Protein transport protein Sec16B Source.54: DFBPPR19315 ---- Animal proteins ---- Probable ATP-dependent RNA helicase DDX47 Source.55: DFBPPR19472 ---- Animal proteins ---- 26S proteasome non-ATPase regulatory subunit 2 Source.56: DFBPPR19823 ---- Animal proteins ---- Alanine aminotransferase 1 Source.57: DFBPPR20408 ---- Animal proteins ---- Phospholipid scramblase 2 Source.58: DFBPPR21002 ---- Animal proteins ---- Olfactomedin-like protein 2B Source.59: DFBPPR21012 ---- Animal proteins ---- tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6 Source.60: DFBPPR21495 ---- Animal proteins ---- Synaptosomal-associated protein 47 Source.61: DFBPPR21626 ---- Animal proteins ---- Mitochondrial import receptor subunit TOM5 homolog Source.62: DFBPPR21830 ---- Animal proteins ---- Guanine nucleotide exchange factor for Rab-3A Source.63: DFBPPR21905 ---- Animal proteins ---- Tubulin polymerization-promoting protein family member 2 Source.64: DFBPPR22434 ---- Animal proteins ---- UPF0692 protein C19orf54 homolog Source.65: DFBPPR22618 ---- Animal proteins ---- BSD domain-containing protein 1 Source.66: DFBPPR8544 ---- Animal proteins ---- Xaa-Pro aminopeptidase 2 Source.67: DFBPPR8548 ---- Animal proteins ---- Interstitial collagenase Source.68: DFBPPR8896 ---- Animal proteins ---- Heat-stable enterotoxin receptor Source.69: DFBPPR9132 ---- Animal proteins ---- Dystrophin Source.70: DFBPPR10015 ---- Animal proteins ---- Zona pellucida sperm-binding protein 3 Source.71: DFBPPR10051 ---- Animal proteins ---- Cytochrome b-c1 complex subunit Rieske, mitochondrial Source.72: DFBPPR10075 ---- Animal proteins ---- Mast/stem cell growth factor receptor Kit Source.73: DFBPPR10288 ---- Animal proteins ---- Protein-lysine 6-oxidase Source.74: DFBPPR10354 ---- Animal proteins ---- DNA topoisomerase 2-beta Source.75: DFBPPR10442 ---- Animal proteins ---- Neurexin-1 Source.76: DFBPPR10529 ---- Animal proteins ---- Cadherin-20 Source.77: DFBPPR10631 ---- Animal proteins ---- Kinetochore protein Nuf2 Source.78: DFBPPR10807 ---- Animal proteins ---- Dystrophin Source.79: DFBPPR10880 ---- Animal proteins ---- Golgi apparatus protein 1 Source.80: DFBPPR11387 ---- Animal proteins ---- Amphiphysin Source.81: DFBPPR11415 ---- Animal proteins ---- Elongation factor Tu, mitochondrial Source.82: DFBPPR11703 ---- Animal proteins ---- Transcription factor CP2 Source.83: DFBPPR11705 ---- Animal proteins ---- SCO-spondin Source.84: DFBPPR11898 ---- Animal proteins ---- Serine/threonine-protein phosphatase 6 regulatory subunit 3 Source.85: DFBPPR11970 ---- Animal proteins ---- Rap1 GTPase-activating protein 2 Source.86: DFBPPR12788 ---- Animal proteins ---- Macrophage metalloelastase Source.87: DFBPPR12865 ---- Animal proteins ---- Sperm surface protein Sp17 Source.88: DFBPPR12870 ---- Animal proteins ---- Protein transport protein Sec16B Source.89: DFBPPR12928 ---- Animal proteins ---- Regulatory solute carrier protein family 1 member 1 Source.90: DFBPPR13246 ---- Animal proteins ---- Pancreatic triacylglycerol lipase Source.91: DFBPPR13836 ---- Animal proteins ---- Myeloid differentiation primary response protein MyD88 Source.92: DFBPPR14360 ---- Marine protein ---- Phenylalanine--tRNA ligase beta subunit, chloroplastic Source.93: DFBPPR14371 ---- Marine protein ---- DNA-directed RNA polymerase subunit alpha Source.94: DFBPPR14422 ---- Marine protein ---- Translation initiation factor IF-2, chloroplastic Source.95: DFBPPR15031 ---- Microorganism protein ---- NAD-dependent histone deacetylase SIR2 Source.96: DFBPPR15052 ---- Microorganism protein ---- 5'-3' exoribonuclease 2 Source.97: DFBPPR15229 ---- Microorganism protein ---- Glycylpeptide N-tetradecanoyltransferase Source.98: DFBPPR15548 ---- Microorganism protein ---- U3 small nucleolar RNA-associated protein 25 Source.99: DFBPPR7820 ---- Plant protein ---- DNA-directed RNA polymerase subunit beta Source.100: DFBPPR7825 ---- Plant protein ---- Phytochrome C
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Anticancer activity

The authors prepared three peptides derived from the type III connecting segment domain (IIICS) of fibronectin: EILDV, EILDVPST, REDV, and a laminin-related peptide YIGSR. Each peptide inhibited the experimental tumor metastasis of B16-BL6 melanoma, while EILDV had the strongest effect. The peptides conjugated with poly(ethylene glycol) (PEG) were more effective than the unmodified peptides in molar ratio terms. A mixture composed of PEG hybrids with EILDV, REDV and YIGSR significantly inhibited tumor metastasis.

Specific target protein(s)

N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

N[C@@]([H])(CCCNC(=N)N)C(=O)N[C@@]([H])(CCC(=O)O)C(=O)N[C@@]([H])(CC(=O)O)C(=O)N[C@@]([H])(C(C)C)C(=O)O

Preparation method

Mode of preparation	Synthesis
Enzyme(s)/starter culture	No enzyme

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Yamamoto S, Kaneda Y, Okada N, Nakagawa S, Kubo K, Inoue S, Maeda M, Yamashiro Y, Kawasaki K, Mayumi T. Antimetastatic effects of synthetic peptides containing the core sequence of the type III connecting segment domain (IIICS) of fibronectin. Anticancer Drugs. 1994 Aug;5(4):424-8. PMID: 7949246 DOI: 10.1097/00001813-199408000-00006
Other literature(s)	[1] Minkiewicz P, Dziuba J, Iwaniak A, et al. BIOPEP database and other programs for processing bioactive peptide sequences[J]. Journal of Aoac International, 2008, 91(4):965-980.
PubDate	1994