DFBP ID - DFBPANCA0110(Anticancer peptide)

DFBP ID DFBPANCA0110 Peptide sequence FFVAPFPEVFGK Type Native peptide Peptide/Function name Anticancer peptide

Function-activity relationship

Main bioactivity Anticancer activity Otheir bioactivity ACE-inhibitory activity [D1], Antihypertensive activity [D2], Antithrombotic activity [D3], Multifunctional activity [D4]

Calculated physicochemical properties

Three-letter amino acid Phe-Phe-Val-Ala-Pro-Phe-Pro-Glu-Val-Phe-Gly-Lys Single-letter amino acid FFVAPFPEVFGK Peptide length 12 Peptide mass Experimental mass Theoretical mass N.D 1384.63 Da c Net charge 0.00 c Isoelectric point (pI) 6.92 c IC50 N.D pIC50 N.D GRAVY 0.8667 c Hydrophilic residue ratio 83.33% c Peptide calculator To calculate the physicochemical properties of bioactive peptide.

Peptide source & Food-borne protein(s) search

Classification Animal Organism/Source Bovine milk protein Precursor protein αs1-Casein Residue position N.D Precursor protein(s) search Source.1: DFBPPR7717 ---- Milk proteins ---- Alpha-S1-casein Source.2: DFBPPR8488 ---- Milk proteins ---- Alpha-S1-casein Link-research Inductive analysis There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Anticancer activity The peptide showed anticancer activity. Specific target protein(s) N.D

Taste properties & Structure

Bitterness Literature report N.D Bitter prediction tools Bitter taste prediction SMILES N[C@@]([H])(Cc1ccccc1)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)N[C@@]([H])(C(C)C)C(=O)N[C@@]([H])(C)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CCC(=O)O)C(=O)N[C@@]([H])(C(C)C)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)NCC(=O)N[C@@]([H])(CCCCN)C(=O)O Structure Docking

Preparation method

Mode of preparation Enzymatic hydrolysis Enzyme(s)/starter culture Bovine milk protiens were hydrolyzed with trypsin.

Stability & Cytotoxicity

Peptide stability Literature report: N.D EHP-Tool: Enzymatic Hydrolysis Prediction Tool (EHP-Tool) Peptide cytotoxicity Literature report: N.D Prediction: ToxinPred

Additional information

Additional information N.D

Database cross-references

DFBP [D1] DFBPACEI0331 [D2] DFBPANHY0180, DFBPANHY0888 [D3] DFBPANTH0151 [D4] DFBPMUFU0097

BIOPEP-UWM [D5] 7800, 8311

APD [D6] -

BioPepDB [D7] -

MBPDB [D8] -

Reference(s)

Primary literature Juillerat-Jeanneret L, Robert MC, Juillerat MA. Peptides from Lactobacillus hydrolysates of bovine milk caseins inhibit prolyl-peptidases of human colon cells. J Agric Food Chem. 2011 Jan 12;59(1):370-7. PMID: 21126072 DOI: 10.1021/jf102803a Other literature(s) N.D PubDate 2011