Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANCA0320(Anticancer peptide)

DFBP ID	DFBPANCA0320
Peptide sequence	LVPK
Type	Native peptide
Peptide/Function name	Anticancer peptide

Function-activity relationship

Main bioactivity	Anticancer activity
Otheir bioactivity	Antioxidative activity ^[D1], Multifunctional activity ^[D2]

Calculated physicochemical properties

Three-letter amino acid

Leu-Val-Pro-Lys

Single-letter amino acid

LVPK

Peptide length

Peptide mass

Experimental mass	Theoretical mass
455.0 Da	455.59 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

10.04 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

0.6250^c

Hydrophilic residue ratio

75%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Plant
Organism/Source	Black soybean (Glycine max (L.) Merr.)
Precursor protein	Black soybean protein
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR0858 ---- Plant proteins ---- Bidirectional sugar transporter SWEET11 Source.2: DFBPPR1048 ---- Plant proteins ---- ABC transporter B family member 25 Source.3: DFBPPR1314 ---- Plant proteins ---- Ferredoxin-dependent glutamate synthase, chloroplastic Source.4: DFBPPR1536 ---- Plant proteins ---- Protein DWARF 53 Source.5: DFBPPR1577 ---- Plant proteins ---- Very-long-chain aldehyde decarbonylase GL1-6 Source.6: DFBPPR1602 ---- Plant proteins ---- SNW/SKI-interacting protein A Source.7: DFBPPR2314 ---- Plant proteins ---- Phosphoacetylglucosamine mutase Source.8: DFBPPR2343 ---- Plant proteins ---- Protein kinase G11A Source.9: DFBPPR2483 ---- Plant proteins ---- P-loop NTPase domain-containing protein LPA1 Source.10: DFBPPR2603 ---- Plant proteins ---- Disease resistance protein Pik-2 Source.11: DFBPPR2657 ---- Plant proteins ---- E3 SUMO-protein ligase SIZ1 Source.12: DFBPPR3084 ---- Plant proteins ---- Probable metal-nicotianamine transporter YSL13 Source.13: DFBPPR3089 ---- Plant proteins ---- E3 SUMO-protein ligase SIZ2 Source.14: DFBPPR3250 ---- Plant proteins ---- Disease resistance protein Pikm2-TS Source.15: DFBPPR3310 ---- Plant proteins ---- Transcription factor PCF2 Source.16: DFBPPR3373 ---- Plant proteins ---- 4-coumarate--CoA ligase-like 1 Source.17: DFBPPR3584 ---- Plant proteins ---- Signal recognition particle 19 kDa protein Source.18: DFBPPR3770 ---- Plant proteins ---- Putative DEAD-box ATP-dependent RNA helicase 51 Source.19: DFBPPR4240 ---- Plant proteins ---- Protein DWARF 53-LIKE Source.20: DFBPPR4283 ---- Plant proteins ---- SCAR-like protein 1 Source.21: DFBPPR4471 ---- Plant proteins ---- Disease resistance protein Piks-2 Source.22: DFBPPR4479 ---- Plant proteins ---- Metal transporter Nramp6 Source.23: DFBPPR4805 ---- Plant proteins ---- B3 domain-containing protein Os02g0764100 Source.24: DFBPPR4865 ---- Plant proteins ---- P-loop NTPase domain-containing protein LPA1 homolog Source.25: DFBPPR4902 ---- Plant proteins ---- Endoribonuclease Dicer homolog 1 Source.26: DFBPPR5454 ---- Plant proteins ---- Ferredoxin-dependent glutamate synthase, chloroplastic Source.27: DFBPPR6240 ---- Plant proteins ---- Stromal processing peptidase, chloroplastic Source.28: DFBPPR6841 ---- Plant proteins ---- Glutathione S-transferase Source.29: DFBPPR7289 ---- Plant proteins ---- Myb-related protein Hv33 Source.30: DFBPPR7495 ---- Plant proteins ---- Fructose-1,6-bisphosphatase, cytosolic Source.31: DFBPPR16043 ---- Animal proteins ---- Adenylate cyclase type 6 Source.32: DFBPPR16473 ---- Animal proteins ---- Glycoprotein hormones alpha chain Source.33: DFBPPR16662 ---- Animal proteins ---- Rab GDP dissociation inhibitor beta Source.34: DFBPPR17092 ---- Animal proteins ---- Plasma membrane calcium-transporting ATPase 4 Source.35: DFBPPR17264 ---- Animal proteins ---- Tubulin-specific chaperone D Source.36: DFBPPR17367 ---- Animal proteins ---- Cyclic GMP-AMP synthase Source.37: DFBPPR17484 ---- Animal proteins ---- Histone H1.8 Source.38: DFBPPR17517 ---- Animal proteins ---- Glycoprotein hormones alpha chain Source.39: DFBPPR17775 ---- Animal proteins ---- Elongator complex protein 3 Source.40: DFBPPR17805 ---- Animal proteins ---- SNW domain-containing protein 1 Source.41: DFBPPR17990 ---- Animal proteins ---- Nuclear factor erythroid 2-related factor 2 Source.42: DFBPPR18049 ---- Animal proteins ---- Transcription factor Dp-1 Source.43: DFBPPR18332 ---- Animal proteins ---- G protein-activated inward rectifier potassium channel 1 Source.44: DFBPPR18498 ---- Animal proteins ---- Neutral cholesterol ester hydrolase 1 Source.45: DFBPPR18533 ---- Animal proteins ---- V-type proton ATPase subunit d 1 Source.46: DFBPPR19002 ---- Animal proteins ---- Mitochondrial basic amino acids transporter Source.47: DFBPPR19052 ---- Animal proteins ---- Rab GDP dissociation inhibitor beta Source.48: DFBPPR19302 ---- Animal proteins ---- Solute carrier family 2, facilitated glucose transporter member 12 Source.49: DFBPPR19310 ---- Animal proteins ---- Chromatin assembly factor 1 subunit A Source.50: DFBPPR19945 ---- Animal proteins ---- Protein maelstrom homolog Source.51: DFBPPR20044 ---- Animal proteins ---- Secernin-1 Source.52: DFBPPR20056 ---- Animal proteins ---- Cyclin-dependent kinases regulatory subunit 1 Source.53: DFBPPR20314 ---- Animal proteins ---- DNA-directed RNA polymerase III subunit RPC3 Source.54: DFBPPR20423 ---- Animal proteins ---- 39S ribosomal protein L16, mitochondrial Source.55: DFBPPR20544 ---- Animal proteins ---- 28S ribosomal protein S34, mitochondrial Source.56: DFBPPR20833 ---- Animal proteins ---- Src kinase-associated phosphoprotein 2 Source.57: DFBPPR21078 ---- Animal proteins ---- Guanine nucleotide-binding protein-like 3-like protein Source.58: DFBPPR21528 ---- Animal proteins ---- Vasculin Source.59: DFBPPR21662 ---- Animal proteins ---- Ornithine decarboxylase antizyme 1 Source.60: DFBPPR22454 ---- Animal proteins ---- R3H domain-containing protein 4 Source.61: DFBPPR22578 ---- Animal proteins ---- Protein FAM71F1 Source.62: DFBPPR8727 ---- Animal proteins ---- Cyclic GMP-AMP synthase Source.63: DFBPPR9151 ---- Animal proteins ---- Glycoprotein hormones alpha chain Source.64: DFBPPR9296 ---- Animal proteins ---- Transcobalamin-1 Source.65: DFBPPR9343 ---- Animal proteins ---- Alpha-1-antitrypsin Source.66: DFBPPR9560 ---- Animal proteins ---- Protein maelstrom homolog Source.67: DFBPPR9722 ---- Animal proteins ---- Fetal and adult testis-expressed transcript protein homolog Source.68: DFBPPR9773 ---- Animal proteins ---- Rab GDP dissociation inhibitor beta Source.69: DFBPPR10052 ---- Animal proteins ---- Protein Wnt-11 Source.70: DFBPPR10055 ---- Animal proteins ---- Protein Wnt-3a Source.71: DFBPPR10059 ---- Animal proteins ---- Protein Wnt-4 Source.72: DFBPPR10504 ---- Animal proteins ---- Elongator complex protein 3 Source.73: DFBPPR10599 ---- Animal proteins ---- Chromosome-associated kinesin KIF4 Source.74: DFBPPR10652 ---- Animal proteins ---- Formimidoyltransferase-cyclodeaminase Source.75: DFBPPR10788 ---- Animal proteins ---- D-aminoacyl-tRNA deacylase 2 Source.76: DFBPPR11625 ---- Animal proteins ---- Tripartite motif-containing protein 59 Source.77: DFBPPR11669 ---- Animal proteins ---- V-type proton ATPase subunit d 2 Source.78: DFBPPR11681 ---- Animal proteins ---- Ornithine decarboxylase antizyme 1 Source.79: DFBPPR12347 ---- Animal proteins ---- Progesterone receptor Source.80: DFBPPR12384 ---- Animal proteins ---- Calcium-independent phospholipase A2-gamma Source.81: DFBPPR12448 ---- Animal proteins ---- Dimethylaniline monooxygenase [N-oxide-forming] 1 Source.82: DFBPPR12531 ---- Animal proteins ---- Solute carrier family 12 member 4 Source.83: DFBPPR12691 ---- Animal proteins ---- Glycoprotein hormones alpha chain Source.84: DFBPPR13322 ---- Animal proteins ---- Glycoprotein hormones alpha chain Source.85: DFBPPR13473 ---- Animal proteins ---- Glycoprotein hormones alpha chain Source.86: DFBPPR13631 ---- Animal proteins ---- Glycoprotein hormones alpha chain Source.87: DFBPPR14001 ---- Animal proteins ---- Glycoprotein hormones alpha chain 1 Source.88: DFBPPR14013 ---- Animal proteins ---- Glycoprotein hormones alpha chain 2 Source.89: DFBPPR14196 ---- Marine protein ---- Mini-chromosome maintenance complex-binding protein Source.90: DFBPPR14243 ---- Marine protein ---- Glycoprotein hormones alpha chain 2 Source.91: DFBPPR14246 ---- Marine protein ---- Glycoprotein hormones alpha chain 1 Source.92: DFBPPR14517 ---- Marine protein ---- Uncharacterized protein ycf54 Source.93: DFBPPR14869 ---- Marine protein ---- Glycoprotein hormones alpha chain Source.94: DFBPPR15178 ---- Microorganism protein ---- Phosphoglycerate kinase Source.95: DFBPPR15266 ---- Microorganism protein ---- Phosphatidylethanolamine N-methyltransferase Source.96: DFBPPR15342 ---- Microorganism protein ---- Histone transcription regulator 3 homolog Source.97: DFBPPR15366 ---- Microorganism protein ---- DNA-directed RNA polymerases I, II, and III subunit RPABC1 Source.98: DFBPPR15713 ---- Microorganism protein ---- ATPase expression protein 1, mitochondrial Source.99: DFBPPR7942 ---- Plant protein ---- Polyphenol oxidase A1, chloroplastic
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Anticancer activity

The cancer cell antiproliferative activity of peptide fractions from black soybean protein was evaluated using MTT colorimetric assay. The final purified peptide fraction (F2-c) showed high cytotoxic potential against human liver (HepG2), lung (MCF-7) and cervical (Hela) cancer cells with the IC₅₀ values of 0.22, 0.15 and 0.32 μM, respectively. The amino acid sequence of F2-c was identified as Leu/Ile-Val-Pro-Lys (L/I-VPK).
Molecular docking studies revealed that the purified peptides effectively bound with four apoptosis related key proteins (XIAP, caspase-3, caspase-7, and Bcl-2) via hydrophobic effects and hydrogen bonds, amongst them, the peptide-caspase-3 binding showed the strongest binding energy.
The 3D structure of the BIR3 domain of XIAP (PDB code: 1g3f), the crystal structures of heterotetrameric active caspase-3 (PDB ID 1RHM) in complex with a nicotinic acid aldehyde inhibitor and unliganded caspase-7 (PDB ID 3IBF), the chain A of structure of Bcl-2 (PDB ID 2XA0) were derived from the RCSB PDB Protein Data Bank (http:// www.rcsb.org/pdb/home/home.do).

Specific target protein(s)

N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(C(C)C)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CCCCN)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	The protein hydrolysate was obtained by enzyme hydrolysis method with alcalase (EC 3.4.21.1, 20 × 10⁴ U/g).

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

The peptide fraction Leu/Ile-Val-Pro-Lys from black soybean byproduct with significant antioxidant and anticancer activities could be a good candidate for functional foods or related drugs.

Database cross-references

DFBP

[D1]	DFBPANOX1135
[D2]	DFBPMUFU0697

[D3]

[D4]

[D5]

[D6]

Reference(s)

Primary literature	Chen, Z., Li, W., Santhanam, R.K., Wang, C., Gao, X., Chen, Y., et al. Bioactive peptide with antioxidant and anticancer activities from black soybean [Glycine max (L.) Merr.] byproduct: isolation, identification and molecular docking study. European Food Research and Technology. 2018, 245, 677-89. DOI: 10.1007/s00217-018-3190-5
Other literature(s)	N.D
PubDate	2018