Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANCA0332(Anticancer peptide)

DFBP ID	DFBPANCA0332
Peptide sequence	GLSDGEWQ
Type	Native peptide
Peptide/Function name	Anticancer peptide

Function-activity relationship

Main bioactivity	Anticancer activity
Otheir bioactivity	ACE-inhibitory activity ^[D1], Antimicrobial activity ^[D2], Multifunctional activity ^[D3]

Calculated physicochemical properties

Three-letter amino acid

Gly-Leu-Ser-Asp-Gly-Glu-Trp-Gln

Single-letter amino acid

GLSDGEWQ

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	890.90 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

3.04 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-1.1500^c

Hydrophilic residue ratio

50%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Beef sarcoplasmic
Precursor protein	Beef sarcoplasmic protein hydrolysates
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR16534 ---- Animal proteins ---- Myoglobin Source.2: DFBPPR18268 ---- Animal proteins ---- Myoglobin Source.3: DFBPPR9497 ---- Animal proteins ---- Myoglobin Source.4: DFBPPR13323 ---- Animal proteins ---- Myoglobin Source.5: DFBPPR13812 ---- Animal proteins ---- Myoglobin
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Anticancer activity

The effect of separated peptides on breast cancer (MCF-7), lung cancer (A549), and stomach cancer (AGS) cell viability was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Peptide GLSDGEWQ slightly reduced the cell viability of MCF-7 cell lines (Fig. 1). Peptide GLSDGEWQ showed very strong inhibition of AGS proliferation by 80%, at a dose of 400 μg/ml (Fig. 2). This peptide had no cytotoxic effect on A549 cells (Fig. 3).


Fig. 1. Effect of the synthetic peptide GLSDGEWQ on cell viability of breast cancer cell line (MCF-7).	Fig. 2. Effect of the synthetic peptide GLSDGEWQ on cell viability of stomach cancer cell line (AGS).	Fig. 3. Effect of the synthetic peptide GLSDGEWQ on cell viability of human lung cancer cell line (A549).

Specific target protein(s)

N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

NCC(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(CO)C(=O)N[C@@]([H])(CC(=O)O)C(=O)NCC(=O)N[C@@]([H])(CCC(=O)O)C(=O)N[C@@]([H])(CC(=CN2)C1=C2C=CC=C1)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Hydrolysis with commercial enzymes (thermolysin+proteinase A).

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

The ACE inhibitory peptides identified from beef sarcoplasmic protein hydrolysates have both antimicrobial and cancer cell cytotoxic effects. The ACE inhibitory peptides can be expected to possess other biological functions and used as functional materials for food industry.

Database cross-references

DFBP

[D1]	DFBPACEI0115
[D2]	DFBPAMIC0514
[D3]	DFBPMUFU0049

[D4]

[D5]

[D6]

[D7]

Reference(s)

Primary literature	Jang, A., Jo, C., Kang, K.-S., Lee, M. Antimicrobial and human cancer cell cytotoxic effect of synthetic angiotensin-converting enzyme (ACE) inhibitory peptides. Food Chem. 2008, 107, 327-36. DOI: 10.1016/j.foodchem.2007.08.036
Other literature(s)	[1] Udenigwe C C , Hannah W , Gong M , et al. Preclinical Evidence on the Anticancer Properties of Food Peptides[J]. Protein Pept Lett, 2017, 24(2):-.
PubDate	2008