Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANCA0337(Anticancer peptide)

DFBP ID	DFBPANCA0337
Peptide sequence	LSTAADMQGVTDGASGASKDYLKPDD
Type	Native peptide
Peptide/Function name	Anticancer peptide

Function-activity relationship

Main bioactivity	Anticancer activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Leu-Ser-Thr-Ala-Ala-Asp-Met-Gln-Gly-Val-Thr-Asp-Gly-Ala-Ser-Gly-Ala-Ser-Lys-Asp-Tyr-Leu-Lys-Pro-Asp-Asp

Single-letter amino acid

LSTAADMQGVTDGASGASKDYLKPDD

Peptide length

Peptide mass

Experimental mass	Theoretical mass
2914 Da	2613.75 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

3.73 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-0.6077^c

Hydrophilic residue ratio

46.15%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Bacteria
Organism/Source	Pseudomonas aeruginosa
Precursor protein	Pseudomonas aeruginosa azurin
Residue position	N.D
Precursor protein(s) search	No matching precursor protein found
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Anticancer activity	The p28 peptide is an extremely promising peptide for its anticancer capability as due to a direct interaction with the tumor suppressor p53.
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

N.D

Preparation method

Mode of preparation	Synthesis
Enzyme(s)/starter culture	N.D

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Cantini F, Gianni' P, Savarin P, Bizzarri AR, Sette M. Solution structure of the anticancer p28 peptide in biomimetic medium. J Pept Sci. 2021 Nov;27(11):e3357. PMID: 34151482 DOI: 10.1002/psc.3357
Other literature(s)	[1] Anna Rita Bizzarri, Ilaria Moscetti, Salvatore Cannistraro, Interaction of the anticancer p28 peptide with p53-DBD as studied by fluorescence, FRET, docking and MD simulations, Biochimica et Biophysica Acta (BBA) - General Subjects, Volume 1863, Issue 2, 2019, Pages 342-350, ISSN 0304-4165.
PubDate	2021