DFBP ID - DFBPANCA0340(Anticancer peptide)

DFBP ID DFBPANCA0340 Peptide sequence FIHHIIGGLFSAGKAIHRLIRRRRR Type Native peptide Peptide/Function name Anticancer peptide

Function-activity relationship

Main bioactivity Anticancer activity Otheir bioactivity N.D

Calculated physicochemical properties

Three-letter amino acid Phe-Ile-His-His-Ile-Ile-Gly-Gly-Leu-Phe-Ser-Ala-Gly-Lys-Ala-Ile-His-Arg-Leu-Ile-Arg-Arg-Arg-Arg-Arg Single-letter amino acid FIHHIIGGLFSAGKAIHRLIRRRRR Peptide length 25 Peptide mass Experimental mass Theoretical mass N.D 2981.55 Da c Net charge 8.00 Isoelectric point (pI) 14.00 IC50 N.D pIC50 N.D GRAVY -0.1280 c Hydrophilic residue ratio 56% c Peptide calculator To calculate the physicochemical properties of bioactive peptide.

Peptide source & Food-borne protein(s) search

Classification Animal Organism/Source Oreochromis niloticus Precursor protein N.D Residue position N.D Precursor protein(s) search No matching precursor protein found Link-research Inductive analysis Link 1: DFBPANCA0545----Nile Tilapia, Oreochromis niloticus----Sythesis peptide

Biological/Functional activity & target protein

Anticancer activity TP4 displayed strong anticancer activity in human synovial sarcoma cells by disrupting oxidative status, promoting mitochondrial hyperpolarization and causing calcium overload. Specific target protein(s) N.D

Taste properties & Structure

Bitterness Literature report N.D Bitter prediction tools No prediction can be made about the peptide bitterness. prediction SMILES N.D

Preparation method

Mode of preparation Synthesis Enzyme(s)/starter culture N.D

Stability & Cytotoxicity

Peptide stability Literature report: N.D EHP-Tool: Enzymatic Hydrolysis Prediction Tool (EHP-Tool) Peptide cytotoxicity Literature report: N.D Prediction: ToxinPred

Additional information

Additional information N.D

Database cross-references

BIOPEP-UWM [D1] -

APD [D2] -

BioPepDB [D3] -

MBPDB [D4] -

Reference(s)

Primary literature Su BC, Hung GY, Tu YC, Yeh WC, Lin MC, Chen JY. Marine Antimicrobial Peptide TP4 Exerts Anticancer Effects on Human Synovial Sarcoma Cells via Calcium Overload, Reactive Oxygen Species Production and Mitochondrial Hyperpolarization. Mar Drugs. 2021 Feb 5;19(2):93. PMID: PMC7915578 DOI: 10.3390/md19020093 Other literature(s) [1] Peng KC, Lee SH, Hour AL, Pan CY, Lee LH, Chen JY. Five different piscidins from Nile tilapia, Oreochromis niloticus: analysis of their expressions and biological functions. PLoS One, 2012, 7, e50263. [2] Kuo HM, Tseng CC, Chen NF, Tai MH, Hung HC, Feng CW, Cheng SY, Huang SY, Jean YH, Wen ZH. MSP-4, an Antimicrobial Peptide, Induces Apoptosis via Activation of Extrinsic Fas/FasL- and Intrinsic Mitochondria-Mediated Pathways in One Osteosarcoma Cell Line. Mar Drugs. 2018 Jan 2;16(1):8. PubDate 2021