DFBP ID - DFBPANCA0545(Anticancer peptide)

DFBP ID DFBPANCA0545 Peptide sequence FIHHIIGGLFSAGKAIHRLIRRRRR Type Native peptide Peptide/Function name Anticancer peptide, Tilapia piscidin 4 (TP4)

Function-activity relationship

Main bioactivity Anticancer activity Otheir bioactivity N.D

Calculated physicochemical properties

Three-letter amino acid Phe-Ile-His-His-Ile-Ile-Gly-Gly-Leu-Phe-Ser-Ala-Gly-Lys-Ala-Ile-His-Arg-Leu-Ile-Arg-Arg-Arg-Arg-Arg Single-letter amino acid FIHHIIGGLFSAGKAIHRLIRRRRR Peptide length 25 Peptide mass Experimental mass Theoretical mass N.D 2981.55 Da c Net charge 0.00 c Isoelectric point (pI) 13.21 c IC50 N.D pIC50 N.D GRAVY -0.1280 c Hydrophilic residue ratio 56% c Peptide calculator To calculate the physicochemical properties of bioactive peptide.

Peptide source & Food-borne protein(s) search

Classification Animal Organism/Source Nile Tilapia, Oreochromis niloticus Precursor protein Sythesis peptide Residue position N.D Precursor protein(s) search No matching precursor protein found Link-research Inductive analysis Link 1: DFBPANCA0340----Oreochromis niloticus----N.D

Biological/Functional activity & target protein

Anticancer activity TP4 doses below 10 μg/mL were not detrimental to either synovial sarcoma cell line. The IC50 values were 29.20 and 32.41 μg/mL in SW982 and AsKa-SS cells, respectively. Mechanism: TP4 induces mitochondrial hyperpolarization and subsequent elevation of mitochondrial ROS. Specific target protein(s) N.D

Taste properties & Structure

Bitterness Literature report N.D Bitter prediction tools No prediction can be made about the peptide bitterness. prediction SMILES N.D

Preparation method

Mode of preparation Sythesis peptide Enzyme(s)/starter culture Tilapia piscidin 4 (TP4; H-FIHHIIGGLFSAGKAIHRLIRRRRROH) peptide was synthesized by GL Biochem (Shanghai, China).

Stability & Cytotoxicity

Peptide stability Literature report: N.D EHP-Tool: Enzymatic Hydrolysis Prediction Tool (EHP-Tool) Peptide cytotoxicity Literature report: N.D Prediction: ToxinPred

Additional information

Additional information N.D

Database cross-references

BIOPEP-UWM [D1] -

APD [D2] -

BioPepDB [D3] -

MBPDB [D4] -

Reference(s)

Primary literature Su BC, Hung GY, Tu YC, Yeh WC, Lin MC, Chen JY. Marine Antimicrobial Peptide TP4 Exerts Anticancer Effects on Human Synovial Sarcoma Cells via Calcium Overload, Reactive Oxygen Species Production and Mitochondrial Hyperpolarization. Mar Drugs. 2021 Feb 5;19(2):93. PMID: PMC7915578 DOI: 10.3390/md19020093 Other literature(s) N.D PubDate 2021