Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANCA0651(Anticancer peptide)

DFBP ID	DFBPANCA0651
Peptide sequence	YVPFP
Type	Native peptide
Peptide/Function name	Anticancer peptide, α_S1-Casomorphin

Function-activity relationship

Main bioactivity	Anticancer activity
Otheir bioactivity	Opioid activity ^[D1]

Calculated physicochemical properties

Three-letter amino acid

Tyr-Val-Pro-Phe-Pro

Single-letter amino acid

YVPFP

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	621.73 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

5.92 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

0.5000^c

Hydrophilic residue ratio

80%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Human α_S1-casein
Precursor protein	α_S1-casein
Residue position	158-162
Precursor protein(s) search	Source.1: DFBPPR1946 ---- Plant proteins ---- Phytochrome A Source.2: DFBPPR5615 ---- Plant proteins ---- Regulatory protein viviparous-1 Source.3: DFBPPR5698 ---- Plant proteins ---- Phytochrome A Source.4: DFBPPR7602 ---- Milk proteins ---- Alpha-S1-casein Source.5: DFBPPR7723 ---- Plant proteins ---- Phytochrome A type 3 Source.6: DFBPPR7726 ---- Plant proteins ---- Phytochrome A type 4 Source.7: DFBPPR7727 ---- Plant proteins ---- Phytochrome A type 5 Source.8: DFBPPR22754 ---- Animal proteins ---- Uncharacterized protein C1orf158 homolog Source.9: DFBPPR7814 ---- Plant protein ---- Phytochrome a
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Anticancer activity	α_S1-Casomorphin at very low concentrations produced dramatic changes in cell proliferation (Figure 4, left panel), inhibiting cell growth by 80%.
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(Cc1ccc(O)cc1)C(=O)N[C@@]([H])(C(C)C)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)N1[C@@]([H])(CCC1)C(=O)O

Preparation method

Mode of preparation	Sythesis peptide
Enzyme(s)/starter culture	α_S1-Casomorphin (Tyr-Val-Pro-Phe-Pro) was synthesized by conventional peptide chemistry methods.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

DFBP

[D1]	DFBPOPIO0038

[D2]

[D3]

[D4]

[D5]

Reference(s)

Primary literature	Kampa M, Loukas S, Hatzoglou A, Martin P, Martin PM, Castanas E. Identification of a novel opioid peptide (Tyr-Val-Pro-Phe-Pro) derived from human alpha S1 casein (alpha S1-casomorphin, and alpha S1-casomorphin amide). Biochem J. 1996 Nov 1;319 ( Pt 3)(Pt 3):903-8. PMID: 8920997 DOI: 10.1042/bj3190903
Other literature(s)	N.D
PubDate	1996