Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANHY0003(Antihypertensive peptide)

DFBP ID	DFBPANHY0003
Peptide sequence	HLPLP
Type	Native peptide
Peptide/Function name	Antihypertensive peptide

Function-activity relationship

Main bioactivity	Antihypertensive activity
Otheir bioactivity	ACE-inhibitory activity ^[D1], Antioxidative activity ^[D2], Multifunctional activity ^[D3]

Calculated physicochemical properties

Three-letter amino acid

His-Leu-Pro-Leu-Pro

Single-letter amino acid

HLPLP

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	575.70 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

7.79 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

0.2400^c

Hydrophilic residue ratio

80%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Milk protein
Precursor protein	β-Casein
Residue position	f(134-138)
Precursor protein(s) search	Source.1: DFBPPR1798 ---- Plant proteins ---- UMP-CMP kinase 2 Source.2: DFBPPR3187 ---- Plant proteins ---- Probable glucuronosyltransferase Os10g0205300 Source.3: DFBPPR4827 ---- Plant proteins ---- BURP domain-containing protein 1 Source.4: DFBPPR7605 ---- Milk proteins ---- Beta-casein Source.5: DFBPPR7665 ---- Milk proteins ---- Beta-casein Source.6: DFBPPR7692 ---- Milk proteins ---- Beta-casein Source.7: DFBPPR7700 ---- Milk proteins ---- Beta-casein Source.8: DFBPPR7718 ---- Milk proteins ---- Beta-casein Source.9: DFBPPR8489 ---- Milk proteins ---- Beta-casein Source.10: DFBPPR16229 ---- Animal proteins ---- 5-hydroxytryptamine receptor 1A Source.11: DFBPPR16270 ---- Animal proteins ---- AP-4 complex subunit mu-1 Source.12: DFBPPR17683 ---- Animal proteins ---- Cyanocobalamin reductase / alkylcobalamin dealkylase Source.13: DFBPPR19085 ---- Animal proteins ---- AP-4 complex subunit mu-1 Source.14: DFBPPR21483 ---- Animal proteins ---- F-box/LRR-repeat protein 8 Source.15: DFBPPR13225 ---- Animal proteins ---- 5-hydroxytryptamine receptor 1A Source.16: DFBPPR15017 ---- Microorganism protein ---- Serine/threonine-protein kinase TEL1
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antihypertensive activity

In vitro experiments	Dose (mg/kg)	SBP Decreases	Times
SHR	7	21.1 ± 3.4 mmHg (p < 0.001)	2 h
		≈ 20 mmHg	4 h
		≈ 18 mmHg	6 h
		≈ 10 mmHg	8 h
		≈ 6 mmHg	24 h

Specific target protein(s)

N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(CC1=CN=C-N1)C(=O)N[C@@]([H])(CC(C)C)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CC(C)C)C(=O)N1[C@@]([H])(CCC1)C(=O)O

Structure

Embedded Mol* Viewer

Docking

Embedded Mol* Viewer

Preparation method

Mode of preparation	Fermentation
Enzyme(s)/starter culture	Milk was fermented with E. faecalis (Enterococcus faecalis).

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	Non-Toxin
Prediction:	ToxinPred

Additional information

The antihypertensive effect of HLPLP can be produced by the concomitant action of the parent peptide and several novel derived fragments (HLPL, LPLP, HLP).

Database cross-references

DFBP

[D1]	DFBPACEI0419, DFBPACEI0418, DFBPACEI2321
[D2]	DFBPANOX0710
[D3]	DFBPMUFU0116

[D4]

[D5]

[D6]

[D7]

Reference(s)

Primary literature	Sánchez-Rivera, L., Santos, P.F., Miralles, B., Carrón, R., José Montero, M., Recio, I. Peptide fragments from β-casein f(134–138), HLPLP, generated by the action of rat blood plasma peptidases show potent antihypertensive activity. Food Research International. 2016, 88, 348-53. DOI: 10.1016/j.foodres.2015.12.007
Other literature(s)	[1] Laura SánchezRivera, Ares I , Miralles B , et al. Bioavailability and Kinetics of the Antihypertensive Casein-Derived Peptide HLPLP in Rats[J]. Journal of Agricultural & Food Chemistry, 2014, 62(49):11869. [2] Hernandez-Ledesma, B., M.D. Contreras, and I. Recio, Antihypertensive peptides: Production, bioavailability and incorporation into foods. Advances in Colloid and Interface Science, 2011. 165(1): p. 23-35.
PubDate	2016