Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANHY0004(Antihypertensive peptide)

DFBP ID	DFBPANHY0004
Peptide sequence	HLPL
Type	Native peptide
Peptide/Function name	Antihypertensive peptide

Function-activity relationship

Main bioactivity	Antihypertensive activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

His-Leu-Pro-Leu

Single-letter amino acid

HLPL

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	478.58 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

7.79 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

0.7000^c

Hydrophilic residue ratio

75%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Milk protein
Precursor protein	β-Casein
Residue position	f(134-137)
Precursor protein(s) search	Source.1: DFBPPR0921 ---- Plant proteins ---- Very-long-chain aldehyde decarbonylase GL1-5 Source.2: DFBPPR0927 ---- Plant proteins ---- Serine/threonine-protein kinase TOR Source.3: DFBPPR0933 ---- Plant proteins ---- ATP-dependent RNA helicase SUV3, mitochondrial Source.4: DFBPPR1069 ---- Plant proteins ---- Cinnamoyl-CoA reductase 1 Source.5: DFBPPR1077 ---- Plant proteins ---- Hexokinase-9 Source.6: DFBPPR1092 ---- Plant proteins ---- LOB domain-containing protein CRL1 Source.7: DFBPPR1098 ---- Plant proteins ---- Hexokinase-2 Source.8: DFBPPR1542 ---- Plant proteins ---- Very-long-chain aldehyde decarbonylase GL1-1 Source.9: DFBPPR1594 ---- Plant proteins ---- Disease resistance protein Pik-1 Source.10: DFBPPR1798 ---- Plant proteins ---- UMP-CMP kinase 2 Source.11: DFBPPR1896 ---- Plant proteins ---- Laccase-8 Source.12: DFBPPR2008 ---- Plant proteins ---- Putative MYST-like histone acetyltransferase 1 Source.13: DFBPPR2260 ---- Plant proteins ---- Succinate dehydrogenase subunit 3-1, mitochondrial Source.14: DFBPPR2261 ---- Plant proteins ---- Succinate dehydrogenase subunit 3-2, mitochondrial Source.15: DFBPPR2432 ---- Plant proteins ---- DNA-directed RNA polymerase subunit beta' Source.16: DFBPPR2441 ---- Plant proteins ---- Disease resistance protein Pikm1-TS Source.17: DFBPPR2494 ---- Plant proteins ---- Homeobox protein knotted-1-like 3 Source.18: DFBPPR2660 ---- Plant proteins ---- ABC transporter G family member 25 Source.19: DFBPPR2868 ---- Plant proteins ---- Probable 4-coumarate--CoA ligase 2 Source.20: DFBPPR3083 ---- Plant proteins ---- Auxin response factor 7 Source.21: DFBPPR3174 ---- Plant proteins ---- Probable 4-coumarate--CoA ligase 5 Source.22: DFBPPR3187 ---- Plant proteins ---- Probable glucuronosyltransferase Os10g0205300 Source.23: DFBPPR3375 ---- Plant proteins ---- Probable protein phosphatase 2C 1 Source.24: DFBPPR3442 ---- Plant proteins ---- Spermidine synthase 1 Source.25: DFBPPR3985 ---- Plant proteins ---- 4-coumarate--CoA ligase-like 2 Source.26: DFBPPR4148 ---- Plant proteins ---- Disease resistance protein PIK5-NP Source.27: DFBPPR4214 ---- Plant proteins ---- Disease resistance protein Piks-1 Source.28: DFBPPR4487 ---- Plant proteins ---- Spermidine hydroxycinnamoyltransferase 1 Source.29: DFBPPR4647 ---- Plant proteins ---- BURP domain-containing protein 12 Source.30: DFBPPR4827 ---- Plant proteins ---- BURP domain-containing protein 1 Source.31: DFBPPR4904 ---- Plant proteins ---- APETALA2-like protein 2 Source.32: DFBPPR4947 ---- Plant proteins ---- Putative magnesium transporter MRS2-G Source.33: DFBPPR5116 ---- Plant proteins ---- NAD(P)H-quinone oxidoreductase subunit 2 A, chloroplastic Source.34: DFBPPR5129 ---- Plant proteins ---- Protein ADP-ribosyltransferase PARP3 Source.35: DFBPPR5153 ---- Plant proteins ---- NAD(P)H-quinone oxidoreductase subunit 2 B, chloroplastic Source.36: DFBPPR5261 ---- Plant proteins ---- Cytochrome P450 82A2 Source.37: DFBPPR5269 ---- Plant proteins ---- Cytochrome P450 82A4 Source.38: DFBPPR5302 ---- Plant proteins ---- 4-coumarate--CoA ligase 2 Source.39: DFBPPR5344 ---- Plant proteins ---- Nodulin-16 Source.40: DFBPPR5637 ---- Plant proteins ---- Beta-fructofuranosidase 1 Source.41: DFBPPR5774 ---- Plant proteins ---- Nitrate reductase [NAD(P)H] Source.42: DFBPPR5816 ---- Plant proteins ---- DNA-directed RNA polymerase subunit beta' Source.43: DFBPPR6467 ---- Plant proteins ---- Spermidine synthase 2 Source.44: DFBPPR6468 ---- Plant proteins ---- Spermidine synthase 1 Source.45: DFBPPR6482 ---- Plant proteins ---- Cytochrome P450 82A1 Source.46: DFBPPR6807 ---- Plant proteins ---- DNA-directed RNA polymerase subunit beta' Source.47: DFBPPR7039 ---- Plant proteins ---- Nitrate reductase [NAD(P)H] Source.48: DFBPPR7177 ---- Plant proteins ---- DNA-directed RNA polymerase subunit beta' Source.49: DFBPPR7190 ---- Plant proteins ---- Elongation factor 1-alpha Source.50: DFBPPR7605 ---- Milk proteins ---- Beta-casein Source.51: DFBPPR7665 ---- Milk proteins ---- Beta-casein Source.52: DFBPPR7692 ---- Milk proteins ---- Beta-casein Source.53: DFBPPR7700 ---- Milk proteins ---- Beta-casein Source.54: DFBPPR7718 ---- Milk proteins ---- Beta-casein Source.55: DFBPPR8445 ---- Plant proteins ---- Maturase K Source.56: DFBPPR8489 ---- Milk proteins ---- Beta-casein Source.57: DFBPPR8507 ---- Milk proteins ---- Polycystin-2 Source.58: DFBPPR16027 ---- Animal proteins ---- Rho GTPase-activating protein 35 Source.59: DFBPPR16193 ---- Animal proteins ---- Dimethylaniline monooxygenase [N-oxide-forming] 1 Source.60: DFBPPR16229 ---- Animal proteins ---- 5-hydroxytryptamine receptor 1A Source.61: DFBPPR16270 ---- Animal proteins ---- AP-4 complex subunit mu-1 Source.62: DFBPPR16329 ---- Animal proteins ---- Neuropeptide Y receptor type 1 Source.63: DFBPPR16583 ---- Animal proteins ---- Taste receptor type 1 member 3 Source.64: DFBPPR16641 ---- Animal proteins ---- Alpha-(1,3)-fucosyltransferase 10 Source.65: DFBPPR16720 ---- Animal proteins ---- Tudor domain-containing protein 5 Source.66: DFBPPR16980 ---- Animal proteins ---- 3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase FUT3 Source.67: DFBPPR17390 ---- Animal proteins ---- Dual specificity protein phosphatase 10 Source.68: DFBPPR17683 ---- Animal proteins ---- Cyanocobalamin reductase / alkylcobalamin dealkylase Source.69: DFBPPR17838 ---- Animal proteins ---- Lon protease homolog, mitochondrial Source.70: DFBPPR17955 ---- Animal proteins ---- m7GpppX diphosphatase Source.71: DFBPPR18046 ---- Animal proteins ---- NHP2-like protein 1 Source.72: DFBPPR18814 ---- Animal proteins ---- Sulfotransferase 1A1 Source.73: DFBPPR18844 ---- Animal proteins ---- Phosphatidylinositol 4-kinase alpha Source.74: DFBPPR18892 ---- Animal proteins ---- T-cell surface glycoprotein CD5 Source.75: DFBPPR19085 ---- Animal proteins ---- AP-4 complex subunit mu-1 Source.76: DFBPPR19350 ---- Animal proteins ---- Neuropeptide Y receptor type 1 Source.77: DFBPPR19463 ---- Animal proteins ---- Pro-FMRFamide-related neuropeptide VF Source.78: DFBPPR19549 ---- Animal proteins ---- Mitochondrial RNA pseudouridine synthase RPUSD4 Source.79: DFBPPR19612 ---- Animal proteins ---- Lysosomal-trafficking regulator Source.80: DFBPPR19729 ---- Animal proteins ---- Transmembrane protein 106B Source.81: DFBPPR19905 ---- Animal proteins ---- Complement component C6 Source.82: DFBPPR20223 ---- Animal proteins ---- Protein cereblon Source.83: DFBPPR20273 ---- Animal proteins ---- Uridine diphosphate glucose pyrophosphatase NUDT14 Source.84: DFBPPR20355 ---- Animal proteins ---- RING finger protein 10 Source.85: DFBPPR20451 ---- Animal proteins ---- Alpha-(1,3)-fucosyltransferase 10 Source.86: DFBPPR20588 ---- Animal proteins ---- CXXC-type zinc finger protein 5 Source.87: DFBPPR21483 ---- Animal proteins ---- F-box/LRR-repeat protein 8 Source.88: DFBPPR21536 ---- Animal proteins ---- Alpha-1-acid glycoprotein Source.89: DFBPPR21563 ---- Animal proteins ---- RWD domain-containing protein 3 Source.90: DFBPPR21766 ---- Animal proteins ---- Dehydrogenase/reductase SDR family member 12 Source.91: DFBPPR21767 ---- Animal proteins ---- Tudor domain-containing protein 5 Source.92: DFBPPR21782 ---- Animal proteins ---- Surfactant-associated protein 2 Source.93: DFBPPR21813 ---- Animal proteins ---- Ribosomal RNA processing protein 36 homolog Source.94: DFBPPR22013 ---- Animal proteins ---- DDB1- and CUL4-associated factor 4 Source.95: DFBPPR22035 ---- Animal proteins ---- Protein CCSMST1 Source.96: DFBPPR22188 ---- Animal proteins ---- ER membrane protein complex subunit 8 Source.97: DFBPPR22252 ---- Animal proteins ---- Glutamine amidotransferase-like class 1 domain-containing protein 1 Source.98: DFBPPR22302 ---- Animal proteins ---- Protein angel homolog 2 Source.99: DFBPPR22343 ---- Animal proteins ---- Protein RRNAD1 Source.100: DFBPPR22517 ---- Animal proteins ---- F-box only protein 15 Source.101: DFBPPR22622 ---- Animal proteins ---- Coiled-coil domain-containing glutamate-rich protein 1 Source.102: DFBPPR22733 ---- Animal proteins ---- Armadillo-like helical domain containing protein 1 Source.103: DFBPPR8716 ---- Animal proteins ---- Thyroid peroxidase Source.104: DFBPPR8749 ---- Animal proteins ---- E3 SUMO-protein ligase EGR2 Source.105: DFBPPR8992 ---- Animal proteins ---- Hyaluronidase-3 Source.106: DFBPPR9165 ---- Animal proteins ---- Solute carrier family 22 member 2 Source.107: DFBPPR9206 ---- Animal proteins ---- Serine/threonine-protein phosphatase 1 regulatory subunit 10 Source.108: DFBPPR9445 ---- Animal proteins ---- Securin Source.109: DFBPPR9609 ---- Animal proteins ---- Neuropeptide Y receptor type 1 Source.110: DFBPPR9610 ---- Animal proteins ---- Solute carrier family 22 member 1 Source.111: DFBPPR9746 ---- Animal proteins ---- Valine--tRNA ligase, mitochondrial Source.112: DFBPPR9884 ---- Animal proteins ---- Cartilage intermediate layer protein 1 Source.113: DFBPPR9956 ---- Animal proteins ---- Fatty acid synthase Source.114: DFBPPR9989 ---- Animal proteins ---- VIP peptides Source.115: DFBPPR10080 ---- Animal proteins ---- High affinity nerve growth factor receptor Source.116: DFBPPR10475 ---- Animal proteins ---- Adenylate cyclase type 9 Source.117: DFBPPR10596 ---- Animal proteins ---- FERM, ARHGEF and pleckstrin domain-containing protein 1 Source.118: DFBPPR10603 ---- Animal proteins ---- Collagen alpha-3(VI) chain Source.119: DFBPPR10620 ---- Animal proteins ---- Centromere protein T Source.120: DFBPPR11013 ---- Animal proteins ---- Band 3 anion transport protein Source.121: DFBPPR11399 ---- Animal proteins ---- Probable glutamate--tRNA ligase, mitochondrial Source.122: DFBPPR11473 ---- Animal proteins ---- G2/M phase-specific E3 ubiquitin-protein ligase Source.123: DFBPPR11506 ---- Animal proteins ---- Homeobox protein BarH-like 1b Source.124: DFBPPR11571 ---- Animal proteins ---- Alpha-(1,3)-fucosyltransferase 10 Source.125: DFBPPR11810 ---- Animal proteins ---- Homeobox protein BarH-like 1 Source.126: DFBPPR11878 ---- Animal proteins ---- Prolyl endopeptidase-like Source.127: DFBPPR11933 ---- Animal proteins ---- M-protein, striated muscle Source.128: DFBPPR12323 ---- Animal proteins ---- Flavin-containing monooxygenase 5 Source.129: DFBPPR12384 ---- Animal proteins ---- Calcium-independent phospholipase A2-gamma Source.130: DFBPPR12423 ---- Animal proteins ---- Hepatocyte growth factor receptor Source.131: DFBPPR12448 ---- Animal proteins ---- Dimethylaniline monooxygenase [N-oxide-forming] 1 Source.132: DFBPPR12693 ---- Animal proteins ---- Solute carrier family 22 member 1 Source.133: DFBPPR12729 ---- Animal proteins ---- Solute carrier family 22 member 2 Source.134: DFBPPR13225 ---- Animal proteins ---- 5-hydroxytryptamine receptor 1A Source.135: DFBPPR13814 ---- Animal proteins ---- Pro-FMRFamide-related neuropeptide VF Source.136: DFBPPR14516 ---- Marine protein ---- Uncharacterized protein ycf53 Source.137: DFBPPR14581 ---- Marine protein ---- Interferon alpha/beta receptor 2 Source.138: DFBPPR15015 ---- Microorganism protein ---- Postreplication repair E3 ubiquitin-protein ligase RAD18 Source.139: DFBPPR15017 ---- Microorganism protein ---- Serine/threonine-protein kinase TEL1 Source.140: DFBPPR15031 ---- Microorganism protein ---- NAD-dependent histone deacetylase SIR2 Source.141: DFBPPR15119 ---- Microorganism protein ---- Protein SEY1 Source.142: DFBPPR15232 ---- Microorganism protein ---- U3 small nucleolar RNA-associated protein 10 Source.143: DFBPPR15378 ---- Microorganism protein ---- IMP-specific 5'-nucleotidase 1 Source.144: DFBPPR15774 ---- Microorganism protein ---- Protein SIA1 Source.145: DFBPPR7809 ---- Plant protein ---- DNA-directed RNA polymerase subunit beta' Source.146: DFBPPR7934 ---- Plant protein ---- Acid beta-fructofuranosidase Source.147: DFBPPR8047 ---- Plant protein ---- Acid beta-fructofuranosidase
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antihypertensive activity

In vitro experiments	Dose (mg/kg)	SBP Decreases	Times
SHR	7	≈ 14 mmHg	2 h
		19.4 ± 3.8 mmHg (p < 0.001)	4 h
		≈ 18 mmHg	6 h
		≈ 10 mmHg	8 h
		≈ 6 mmHg	24 h

Specific target protein(s)

N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(CC1=CN=C-N1)C(=O)N[C@@]([H])(CC(C)C)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CC(C)C)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	The peptide was obtained from the β-casein f(133-138) HLPLP hydrolyzed with plasma peptidases.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	Non-Toxin
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Sánchez-Rivera, L., Santos, P.F., Miralles, B., Carrón, R., José Montero, M., Recio, I. Peptide fragments from β-casein f(134–138), HLPLP, generated by the action of rat blood plasma peptidases show potent antihypertensive activity. Food Research International. 2016, 88, 348-53. DOI: 10.1016/j.foodres.2015.12.007
Other literature(s)	[1] Laura SánchezRivera, Ares I , Miralles B , et al. Bioavailability and Kinetics of the Antihypertensive Casein-Derived Peptide HLPLP in Rats[J]. Journal of Agricultural & Food Chemistry, 2014, 62(49):11869.
PubDate	2016