Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANHY0049(Antihypertensive peptide)

DFBP ID	DFBPANHY0049
Peptide sequence	WALKGYK
Type	Native peptide
Peptide/Function name	Antihypertensive peptide, TMP

Function-activity relationship

Main bioactivity	Antihypertensive activity
Otheir bioactivity	ACE-inhibitory activity ^[D1], Antioxidative activity ^[D2], Multifunctional activity ^[D3]

Calculated physicochemical properties

Three-letter amino acid

Trp-Ala-Leu-Lys-Gly-Tyr-Lys

Single-letter amino acid

WALKGYK

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	865.04 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

10.18 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-0.6857^c

Hydrophilic residue ratio

57.14%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Edible fungus
Organism/Source	Mushroom (Tricholoma matsutake)
Precursor protein	Fruiting body
Residue position	N.D
Precursor protein(s) search	No matching precursor protein found
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antihypertensive activity

In vitro experiments	Dose (mg/kg)	SBP Decreases	Times
SHR	25	18 mmHg (p < 0.05)	2 h
		≈ 10 mmHg	4 h
		≈ 11 mmHg	6 h
		≈ 0 mmHg	8 h
		≈ 0 mmHg	10 h
	50	35 mmHg (p < 0.05)	2 h
		≈ 25 mmHg	4 h
		≈ 26 mmHg	6 h
		≈ 26 mmHg	8 h
		≈ 25 mmHg	10 h

Specific target protein(s)

N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(CC(=CN2)C1=C2C=CC=C1)C(=O)N[C@@]([H])(C)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(CCCCN)C(=O)NCC(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)N[C@@]([H])(CCCCN)C(=O)O

Preparation method

Mode of preparation	Extract
Enzyme(s)/starter culture	The peptide was isolated from the crude water extract of the fruiting bodies of a highly prized mushroom Tricholoma matsutakei.

Stability & Cytotoxicity

Peptide stability

Literature report:	The isolated inhibitory peptide TMP retained ACE inhibitory activity after exposure to different pH values and different temperatures for 2 hours. TMP displayed good thermostability and pH stability.
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	Non-Toxin
Prediction:	ToxinPred

Additional information

T. matsutake can be used as a functional food to help prevent hypertension-associated diseases.

Database cross-references

DFBP

[D1]	DFBPACEI1333
[D2]	DFBPANOX0666
[D3]	DFBPMUFU0324

[D4]

[D5]

[D6]

[D7]

Reference(s)

Primary literature	Geng X, Tian G, Zhang W, Zhao Y, Zhao L, Wang H, Ng TB. A Tricholoma matsutake Peptide with Angiotensin Converting Enzyme Inhibitory and Antioxidative Activities and Antihypertensive Effects in Spontaneously Hypertensive Rats. Sci Rep. 2016 Apr 7;6:24130. PMID: 27052674 DOI: 10.1038/srep24130
Other literature(s)	N.D
PubDate	2016