Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANHY0279(Antihypertensive peptide)

DFBP ID	DFBPANHY0279
Peptide sequence	LKPNM
Type	Native peptide
Peptide/Function name	Antihypertensive peptide

Function-activity relationship

Main bioactivity	Antihypertensive activity
Otheir bioactivity	ACE-inhibitory activity ^[D1], Multifunctional activity ^[D2]

Calculated physicochemical properties

Three-letter amino acid

Leu-Lys-Pro-Asn-Met

Single-letter amino acid

LKPNM

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	601.76 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

10.04 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-0.6600^c

Hydrophilic residue ratio

60%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Dried bonito
Precursor protein	Dried bonito hydrolysates
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR0846 ---- Plant proteins ---- Catalase isozyme B Source.2: DFBPPR2451 ---- Plant proteins ---- Fructose-bisphosphate aldolase 3, cytoplasmic Source.3: DFBPPR2493 ---- Plant proteins ---- Fructose-bisphosphate aldolase 2, cytoplasmic Source.4: DFBPPR4922 ---- Plant proteins ---- Fructose-bisphosphate aldolase 1, cytoplasmic Source.5: DFBPPR5505 ---- Plant proteins ---- Fructose-bisphosphate aldolase, cytoplasmic isozyme Source.6: DFBPPR6485 ---- Plant proteins ---- Fructose-bisphosphate aldolase, cytoplasmic isozyme 2 Source.7: DFBPPR6486 ---- Plant proteins ---- Fructose-bisphosphate aldolase, cytoplasmic isozyme 1 Source.8: DFBPPR19656 ---- Animal proteins ---- Fructose-bisphosphate aldolase B Source.9: DFBPPR10368 ---- Animal proteins ---- Fructose-bisphosphate aldolase B Source.10: DFBPPR10369 ---- Animal proteins ---- Fructose-bisphosphate aldolase B Source.11: DFBPPR10783 ---- Animal proteins ---- Fructose-bisphosphate aldolase C Source.12: DFBPPR11293 ---- Animal proteins ---- RIMS-binding protein 2 Source.13: DFBPPR12248 ---- Animal proteins ---- Fructose-bisphosphate aldolase A Source.14: DFBPPR12573 ---- Animal proteins ---- Fructose-bisphosphate aldolase B Source.15: DFBPPR13678 ---- Animal proteins ---- Fructose-bisphosphate aldolase B Source.16: DFBPPR14109 ---- Marine protein ---- Fructose-bisphosphate aldolase A
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antihypertensive activity

Table 1. Inhibitory activities for antihypertensive activities of peptides following intravenous administration in SHR^[1]
Peptide	Dose (mg/kg)	Antihypertensive activity (△mmHg, i.v.*)	Time
LKPNM	10	-80	6 h
	60	-23
* Decrease of systolic blood pressure after intravenous administration

Specific target protein(s)

N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(CCCCN)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CC(=O)N)C(=O)N[C@@]([H])(CCSC)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	N.D

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	Non-Toxin
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

DFBP

[D1]	DFBPACEI0011
[D2]	DFBPMUFU0003

[D3]

[D4]

[D5]

[D6]

Reference(s)

Primary literature	Iroyukifujita, H., Eiichiyokoyama, K., Yoshikawa, M. Classification and Antihypertensive Activity of Angiotensin I-Converting Enzyme Inhibitory Peptides Derived from Food Proteins. Journal of Food Science. 2000, 65, 564-9. DOI: 10.1111/j.1365-2621.2000.tb16049.x
Other literature(s)	[1] Martinez-Maqueda, D., et al., Antihypertensive peptides from food proteins: a review. Food Funct, 2012. 3(4): p. 350-361.
PubDate	2000