Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANHY0287(Antihypertensive peptide)

DFBP ID	DFBPANHY0287
Peptide sequence	RVPSL
Type	Native peptide
Peptide/Function name	Antihypertensive peptide

Function-activity relationship

Main bioactivity	Antihypertensive activity
Otheir bioactivity	ACE-inhibitory activity ^[D1], Antithrombotic activity ^[D2], α-Amylase inhibitory activity ^[D3], α-Glucosidase inhibitory activity ^[D4], Multifunctional activity ^[D5]

Calculated physicochemical properties

Three-letter amino acid

Arg-Val-Pro-Ser-Leu

Single-letter amino acid

RVPSL

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	570.68 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

11.04 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

0.2200^c

Hydrophilic residue ratio

60%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Egg
Precursor protein	Egg white protein
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR1485 ---- Plant proteins ---- Pheophorbide a oxygenase, chloroplastic Source.2: DFBPPR1866 ---- Plant proteins ---- Probable DNA gyrase subunit A, chloroplastic/mitochondrial Source.3: DFBPPR19041 ---- Animal proteins ---- Presenilins-associated rhomboid-like protein, mitochondrial Source.4: DFBPPR9971 ---- Animal proteins ---- Ovotransferrin Source.5: DFBPPR12059 ---- Animal proteins ---- Solute carrier family 46 member 3
Link-research Inductive analysis	Link 1: DFBPANHY0168----Egg----Ovotransferrin

Biological/Functional activity & target protein

Antihypertensive activity

In vitro experiments	Dose (mg/kg)	SBP Decreases	Times
SHR	50	24.5 ± 0.5 mmHg	—

Specific target protein(s)

N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(CCCNC(=N)N)C(=O)N[C@@]([H])(C(C)C)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CO)C(=O)N[C@@]([H])(CC(C)C)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Hydrolysis with alkaline proteinase Alcalase ^[1].

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	Non-Toxin
Prediction:	ToxinPred

Additional information

(1) It is hoped this study will help our understanding and potential use of RVPSL in the treatment or prevention of hypertension.
(2) The serum Ang II, renin, and aldosterone concentrations of the treatment group were reduced in comparison to those of the negative group.

Database cross-references

DFBP

[D1]	DFBPACEI0159
[D2]	DFBPANTH0093
[D3]	DFBPALAM0016
[D4]	DFBPALGL0007
[D5]	DFBPMUFU0062

[D6]

[D7]

[D8]

[D9]

Reference(s)

Primary literature	Yu Z, Yin Y, Zhao W, Chen F, Liu J. Antihypertensive effect of angiotensin-converting enzyme inhibitory peptide RVPSL on spontaneously hypertensive rats by regulating gene expression of the renin-angiotensin system. J Agric Food Chem. 2014 Jan 29;62(4):912-7. PMID: 24404915 DOI: 10.1021/jf405189y
Other literature(s)	[1] Yu Z, Liu B, Zhao W, et al. Primary and secondary structure of novel ACE-inhibitory peptides from egg white protein.[J]. Food Chemistry, 2012, 133(2):315. [2] Liu J B, Yu Z P, Zhao W Z, et al. Isolation and identification of angiotensin-converting enzyme inhibitory peptides from egg white protein hydrolysates[J]. Food Chemistry, 2010, 122(4):1159-1163. [3] Majumder, K. and J. Wu, Molecular targets of antihypertensive peptides: understanding the mechanisms of action based on the pathophysiology of hypertension. Int J Mol Sci, 2014. 16(1): p. 256-283.
PubDate	2014