Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANHY0299(Antihypertensive peptide)

DFBP ID	DFBPANHY0299
Peptide sequence	IPAGV
Type	Native peptide
Peptide/Function name	Antihypertensive peptide

Function-activity relationship

Main bioactivity	Antihypertensive activity
Otheir bioactivity	ACE-inhibitory activity ^[D1], Antioxidative activity ^[D2], Multifunctional activity ^[D3]

Calculated physicochemical properties

Three-letter amino acid

Ile-Pro-Ala-Gly-Val

Single-letter amino acid

IPAGV

Peptide length

Peptide mass

Experimental mass	Theoretical mass
456.3 Da	455.55 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

5.97 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

1.7000^c

Hydrophilic residue ratio

100%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Plant
Organism/Source	Hemp (Cannabis sativa L.)
Precursor protein	Hemp seed protein
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR1654 ---- Plant proteins ---- Malate dehydrogenase, glyoxysomal Source.2: DFBPPR2105 ---- Plant proteins ---- Cellulose synthase-like protein D2 Source.3: DFBPPR5083 ---- Plant proteins ---- Malate dehydrogenase, glyoxysomal Source.4: DFBPPR6525 ---- Plant proteins ---- Plastocyanin, chloroplastic Source.5: DFBPPR7468 ---- Plant proteins ---- Malate dehydrogenase 2, glyoxysomal Source.6: DFBPPR7471 ---- Plant proteins ---- Malate dehydrogenase 1, glyoxysomal Source.7: DFBPPR7487 ---- Plant proteins ---- Malate dehydrogenase, mitochondrial Source.8: DFBPPR18234 ---- Animal proteins ---- Small nuclear ribonucleoprotein-associated protein B' Source.9: DFBPPR18393 ---- Animal proteins ---- Malate dehydrogenase, mitochondrial Source.10: DFBPPR8778 ---- Animal proteins ---- Malate dehydrogenase, mitochondrial Source.11: DFBPPR8082 ---- Plant protein ---- Plastocyanin
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antihypertensive activity

In vitro experiments	Dose (mg/kg)	SBP Decreases	Times
SHR	30	36 mmHg	4 h

Specific target protein(s)

N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])([C@]([H])(CC)C)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(C)C(=O)NCC(=O)N[C@@]([H])(C(C)C)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Hemp seed proteins was hydrolyzed by simulating gastrointestinal tract digestion (Pepsin and Pancreatin).

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	Non-Toxin
Prediction:	ToxinPred

Additional information

The peptide showed 75% renin inhibition.

Database cross-references

DFBP

[D1]	DFBPACEI1282
[D2]	DFBPANOX0646
[D3]	DFBPMUFU0289

[D4]

[D5]

[D6]

[D7]

Reference(s)

Primary literature	Girgih, A.T., He, R., Malomo, S., Offengenden, M., Wu, J., Aluko, R.E. Structural and functional characterization of hemp seed (Cannabis sativa L.) protein-derived antioxidant and antihypertensive peptides. Journal of Functional Foods. 2014, 6, 384-94. DOI: 10.1016/j.jff.2013.11.005
Other literature(s)	N.D
PubDate	2014