Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANHY0390(Antihypertensive peptide)

DFBP ID	DFBPANHY0390
Peptide sequence	WTFR
Type	Native peptide
Peptide/Function name	Antihypertensive peptide

Function-activity relationship

Main bioactivity	Antihypertensive activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Trp-Thr-Phe-Arg

Single-letter amino acid

WTFR

Peptide length

Peptide mass

Experimental mass	Theoretical mass
609.5 Da	608.69 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

11.04 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-0.8250^c

Hydrophilic residue ratio

50%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Plant
Organism/Source	Buckwheat (Fagopyrum esculentum)
Precursor protein	Buckwheat sprouts
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR1689 ---- Plant proteins ---- Auxin response factor 21 Source.2: DFBPPR2049 ---- Plant proteins ---- Auxin response factor 19 Source.3: DFBPPR2071 ---- Plant proteins ---- Auxin response factor 11 Source.4: DFBPPR2365 ---- Plant proteins ---- Auxin response factor 5 Source.5: DFBPPR2604 ---- Plant proteins ---- Auxin response factor 10 Source.6: DFBPPR8394 ---- Plant proteins ---- 11-beta-hydroxysteroid dehydrogenase A Source.7: DFBPPR8421 ---- Plant proteins ---- 11-beta-hydroxysteroid dehydrogenase B Source.8: DFBPPR15986 ---- Animal proteins ---- Toll-like receptor 2 Source.9: DFBPPR16335 ---- Animal proteins ---- Sodium channel subunit beta-1 Source.10: DFBPPR17347 ---- Animal proteins ---- Phytanoyl-CoA dioxygenase, peroxisomal Source.11: DFBPPR17979 ---- Animal proteins ---- Sodium channel subunit beta-1 Source.12: DFBPPR12771 ---- Animal proteins ---- Sodium channel subunit beta-1
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antihypertensive activity

In vitro experiments	Dose (mg/kg)	SBP Decreases (Maximal)	DBP Decreases (Maximal)
SHR	0.10	29.5 ± 4.1 mm Hg	No decrease
Time	—	3-6 h	—

Specific target protein(s)

N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(CC(=CN2)C1=C2C=CC=C1)C(=O)N[C@@]([H])([C@]([H])(O)C)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)O

Preparation method

Mode of preparation	Fermentation
Enzyme(s)/starter culture	Buckwheat sprouts was fermented with lactobacillus plantarum KT(Biotech Japan, Agano, Japan).

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	Non-Toxin
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Koyama M, Naramoto K, Nakajima T, Aoyama T, Watanabe M, Nakamura K. Purification and identification of antihypertensive peptides from fermented buckwheat sprouts. J Agric Food Chem. 2013 Mar 27;61(12):3013-21. PMID: 23432021 DOI: 10.1021/jf305157y
Other literature(s)	N.D
PubDate	2013