Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANHY0543(Antihypertensive peptide)

DFBP ID	DFBPANHY0543
Peptide sequence	MAIPPKK
Type	Native peptide
Peptide/Function name	Antihypertensive peptide

Function-activity relationship

Main bioactivity	Antihypertensive activity
Otheir bioactivity	ACE-inhibitory activity ^[D1], Antithrombotic activity ^[D2], Multifunctional activity ^[D3]

Calculated physicochemical properties

Three-letter amino acid

Met-Ala-Ile-Pro-Pro-Lys-Lys

Single-letter amino acid

MAIPPKK

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	784.02 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

10.59 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-0.4000^c

Hydrophilic residue ratio

71.43%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Bovine milk protein
Precursor protein	κ-Casein
Residue position	f(106-112)
Precursor protein(s) search	Source.1: DFBPPR7686 ---- Milk proteins ---- Kappa-casein Source.2: DFBPPR7695 ---- Milk proteins ---- Kappa-casein Source.3: DFBPPR7715 ---- Milk proteins ---- Kappa-casein Source.4: DFBPPR8492 ---- Milk proteins ---- Kappa-casein
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antihypertensive activity

Peptide	In vitro experiments	Dose (mg/kg)	SBP decrease
MAIPPKK	SHR	10.0	-28.0 mm Hg

Specific target protein(s)

N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(CCSC)C(=O)N[C@@]([H])(C)C(=O)N[C@@]([H])([C@]([H])(CC)C)C(=O)N1[C@@]([H])(CCC1)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(CCCCN)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Hydrolysis with trypsin (casein macropeptide).

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	Non-Toxin
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

DFBP

[D1]	DFBPACEI0291
[D2]	DFBPANTH0006, DFBPANTH0053, DFBPANTH0162
[D3]	DFBPMUFU0393

[D4]

[D5]

[D6]

[D7]

Reference(s)

Primary literature	Miguel, M., Manso, M.A., López-Fandiño, R., Alonso, M.J., Salaices, M. Vascular effects and antihypertensive properties of κ-casein macropeptide. International Dairy Journal. 2007, 17, 1473-7. DOI: 10.1016/j.idairyj.2007.04.009
Other literature(s)	[1] Park Y W. Bioactive Components in Milk and Dairy Products[M]. 2009. [2] Martinez-Maqueda, D., et al., Antihypertensive peptides from food proteins: a review. Food Funct, 2012. 3(4): p. 350-361. [3] Manso M A, Lópezfandiño R. Angiotensin I converting enzyme-inhibitory activity of bovine, ovine, and caprine kappa-casein macropeptides and their tryptic hydrolysates.[J]. Journal of Food Protection, 2003, 66(9):1686.
PubDate	2007