Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANHY0558(Antihypertensive peptide)

DFBP ID	DFBPANHY0558
Peptide sequence	KVLPVP
Type	Native peptide
Peptide/Function name	Antihypertensive peptide

Function-activity relationship

Main bioactivity	Antihypertensive activity
Otheir bioactivity	ACE-inhibitory activity ^[D1], Multifunctional activity ^[D2]

Calculated physicochemical properties

Three-letter amino acid

Lys-Val-Leu-Pro-Val-Pro

Single-letter amino acid

KVLPVP

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	651.84 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

10.04 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

0.8500^c

Hydrophilic residue ratio

83.33%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Milk Protein
Precursor protein	β-Casein
Residue position	f(169-174)
Precursor protein(s) search	Source.1: DFBPPR7665 ---- Milk proteins ---- Beta-casein Source.2: DFBPPR7692 ---- Milk proteins ---- Beta-casein Source.3: DFBPPR7700 ---- Milk proteins ---- Beta-casein Source.4: DFBPPR7718 ---- Milk proteins ---- Beta-casein Source.5: DFBPPR8489 ---- Milk proteins ---- Beta-casein
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antihypertensive activity	The peptide had a strong antihypertensive activity in SHR at a dosage of 1 mg of peptide/kg of BW by 6 h after oral administration (-32.2 ? 1.4 mm Hg; P < 0.001).
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(C(C)C)C(=O)N[C@@]([H])(CC(C)C)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(C(C)C)C(=O)N1[C@@]([H])(CCC1)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Hydrolysis with a proteinase of Lactobacillus helveticus CP790

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	Non-Toxin
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

DFBP

[D1]	DFBPACEI0663
[D2]	DFBPMUFU0147

[D3]

[D4]

[D5]

[D6]

Reference(s)

Primary literature	Maeno, M., Yamamoto, N., Takano, T. Identification of an Antihypertensive Peptide from Casein Hydrolysate Produced by a Proteinase from Lactobacillus helveticus CP790. Journal of Dairy Science. 1996, 79, 1316-21. DOI: 10.3168/jds.S0022-0302(96)76487-1
Other literature(s)	[1] Yamamoto N, Ejiri M, Mizuno S. Biogenic peptides and their potential use[J]. Curr Pharm Des, 2003, 9(16):-. [2] ZsuzsannaBösze. Bioactive Components of Milk[M]. Springer New York, 2008. [3] Martinez-Maqueda, D., et al., Antihypertensive peptides from food proteins: a review. Food Funct, 2012. 3(4): p. 350-361.
PubDate	1996