Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANHY0588(Antihypertensive peptide)

DFBP ID	DFBPANHY0588
Peptide sequence	VKAGF
Type	Native peptide
Peptide/Function name	Antihypertensive peptide, A5

Function-activity relationship

Main bioactivity	Antihypertensive activity
Otheir bioactivity	ACE-inhibitory activity ^[D1], Multifunctional activity ^[D2]

Calculated physicochemical properties

Three-letter amino acid

Val-Lys-Ala-Gly-Phe

Single-letter amino acid

VKAGF

Peptide length

Peptide mass

Experimental mass	Theoretical mass
520.62 Da	520.63 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

10.04 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

0.9000^c

Hydrophilic residue ratio

80%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Pig
Precursor protein	Porcine skeletal myosin B
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR0956 ---- Plant proteins ---- LRR receptor-like serine/threonine-protein kinase SIK1 Source.2: DFBPPR2751 ---- Plant proteins ---- Actin-7 Source.3: DFBPPR2763 ---- Plant proteins ---- Actin-1 Source.4: DFBPPR2989 ---- Plant proteins ---- Actin-2 Source.5: DFBPPR3087 ---- Plant proteins ---- Actin-3 Source.6: DFBPPR5283 ---- Plant proteins ---- Actin-3 Source.7: DFBPPR5841 ---- Plant proteins ---- Actin-1 Source.8: DFBPPR6550 ---- Plant proteins ---- Actin-3 Source.9: DFBPPR6551 ---- Plant proteins ---- Actin-2 Source.10: DFBPPR6552 ---- Plant proteins ---- Actin-1 Source.11: DFBPPR17514 ---- Animal proteins ---- Actin, alpha skeletal muscle Source.12: DFBPPR21756 ---- Animal proteins ---- Probable allantoicase Source.13: DFBPPR9651 ---- Animal proteins ---- Bestrophin-1 Source.14: DFBPPR10319 ---- Animal proteins ---- Actin, alpha cardiac muscle 1 Source.15: DFBPPR10338 ---- Animal proteins ---- Actin, alpha skeletal muscle Source.16: DFBPPR12268 ---- Animal proteins ---- Actin, alpha skeletal muscle Source.17: DFBPPR13996 ---- Animal proteins ---- Actin, alpha skeletal muscle Source.18: DFBPPR15487 ---- Microorganism protein ---- Restriction of telomere capping protein 1 Source.19: DFBPPR7844 ---- Plant protein ---- Actin-1
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antihypertensive activity

In vitro experiments	Dose (mg/kg)	SBP Decreases
SHR	10	12 mmHg	17 mmHg
Time	—	3 h	6 h

Specific target protein(s)

N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	No prediction can be made about the peptide bitterness. ^prediction

SMILES

N[C@@]([H])(C(C)C)C(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(C)C(=O)NCC(=O)N[C@@]([H])(Cc1ccccc1)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Proteins were hydrolyzed with Pepsin.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	Non-Toxin
Prediction:	ToxinPred

Additional information

(1) The peptide may have potential applications as functional food, which could be used as nutraceutical compounds.
(2) A database search for sequence homology showed sequence homology with position 19-23 of actin in rabbit skeletal muscle.

Database cross-references

DFBP

[D1]	DFBPACEI0126, DFBPACEI1549
[D2]	DFBPMUFU0054

[D3]

[D4]

[D5]

[D6]

Reference(s)

Primary literature	Muguruma, M., Ahhmed, A., Katayama, K., Kawahara, S., Maruyama, M., Nakamura, T. Identification of pro-drug type ACE inhibitory peptide sourced from porcine myosin B: Evaluation of its antihypertensive effects in vivo. Food Chem. 2009, 114, 516-22. DOI: 10.1016/j.foodchem.2008.09.081
Other literature(s)	N.D
PubDate	2009